LOCUS       sequence-249960-        9072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  9072 b scaffold from compatibility group 3, made by method II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Engelhardt FAS, Praetorius F, Wachauf CH, Bruggenthies G, Kohler F, 
            Kick B, Kadletz KL, Pham PN, Behler KL, Gerling T, Dietz H
  TITLE     Custom-Size, Functional, and Durable DNA Origami with 
            Design-Specific Scaffolds.
  JOURNAL   ACS Nano. 2019 Apr 22. doi: 10.1021/acsnano.9b01025.
  PUBMED    30990672
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Nano. 
            2019 Apr 22. doi: 10.1021/acsnano.9b01025."
COMMENT     Sequence Label: pre_II_CS3_9072_v1
FEATURES             Location/Qualifiers
     source          1..9072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(70..89)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     280..301
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        464..568
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(569..637,638..1429)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(787..806)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1600..2188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2089..2108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     regulatory      8654..8663
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
       61 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      121 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
      181 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
      241 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
      301 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
      361 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
      421 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      481 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      541 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      601 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      661 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      721 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      781 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      841 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      901 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      961 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1021 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1081 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1141 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1201 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1261 taaatctgga gccggtgagc gtgggtcacg cggtatcatt gcagcactgg ggccagatgg
     1321 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1381 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1441 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1501 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1561 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1621 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1681 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1741 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1801 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1861 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1921 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1981 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2041 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2101 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2161 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2221 ggccttttgc tggccttttg ctcaccttag caagagccgc acgacgacca gaggccagtt
     2281 atccagagtt aggatacctc aatgtgcatc cgctcggttc taaggacatt atttcagtcc
     2341 tttaagatct cccgtataga agcctcacgt tagggggcgc cgtgccttca cgccctccca
     2401 tttaggaata ccttgtctcc gccgtcttta ttcagtagcc ctatgcatta cgatgtggcg
     2461 cttcccccgc gtgggcgcag aaatttactg aggcggattc gaaacgactg tgagggcagg
     2521 ataggtgagc aggcactggc acgtaatcaa ccaacggact cacccgtgtg caggcctaaa
     2581 aacagccctc gaagggcact tggatatgaa tgaacccact tgttttgact cgtggaggcg
     2641 tggttttatt actgtgctca gttaacgccg catgaattta gctctgatca ccgtaagggt
     2701 aactgcacta gacatgttgt gggcatttaa gtcctgcagt atctttttgt taggtggaac
     2761 ggcctagggg taccttccgt gagaaactcc cagatgatgc atgttcgagt acttgtgaaa
     2821 tggatggtcg catcccctcc tctcacacat tacactgtct cgcgcgggtt gcgtcttgac
     2881 cggtacaagt tgtgtaacct tacacctcta gaaacatttt agcagtcgct ccaattgata
     2941 ccacgacctc agcgcgcgtt gggagaccgt tgccaaacct agttttcatg ttttccttca
     3001 aacgtgcgat ataattcctc gcacctagcc gcctagctga gaagtgttat ataggtcact
     3061 tgaaaagagt aggttcagca tatggaactt gggggtttgt tcgctatgcg gtaacgcgtg
     3121 cccctctgac ttgtcgatga ctcccggcgc ggtttcaatg cgggcatgta atcgtcgcga
     3181 acctttactc aggctataaa gcggcataac cagttgtaac taacagaata cagttctctc
     3241 tcaacggcaa ctacaaaata gcatacttga gacctacgac agtagggtcg cgtcaacctg
     3301 tgcaagaaaa agctagaaga gctgaattct gataattaca gacccataca ccgattaacc
     3361 ccgacttata ggcgtaagtg gtgtcccttg catgacccag gccatatcta tgcccccata
     3421 taaaccaggt cgacttccca ctgatcgaac caataaggaa tgatcgcagc ccataaagta
     3481 cggttcaacg attgctgagc ttcagtgagg ttaacagcgg ctggtgcccg tacattgcct
     3541 cgtcgattag cctagtgagc gtcgacagga gctttaactc ctactttcat acgccaccgc
     3601 acagacttca ttgaccacgt atctgagact ccttgtaaac tgactactcc agctccatca
     3661 acaatcttga agaagttcta tccgatcacg gttatcgcga gtcttagcgt agccaatgct
     3721 gggccgtagc gtctacttaa cggaaagaag ggtgaaagct gccacagaaa ataaattact
     3781 atttacattt gtaagacaaa gttagtatgg ttaaatactg gcttagtctg cgggaaactt
     3841 agccatacga cttggtttgc ctatcgttac agcgtacgac ccgttcacca atcgcatttg
     3901 cgatgcagat aacaactcgg aagctgtatg acctctcgag cgttagtcgc agttttagta
     3961 gagaaaaccc gatagccagg cgttcaagcc tattccccat tatgcgtccg ctattgactt
     4021 cgctggaccc tcgcggagtc ggcgacaaat aacatagagg atatcgtcac gggatagcgc
     4081 tctccattgg aattgtacat gagagttgga ctgccgaacg catgggtgga gcagccaagc
     4141 acccggggac tacgaaggtt tccgcgcatg tgttaccttt cagccgaaga gaaggtcggt
     4201 ggatctcgct ctttcggagc tcatactcct catgctacaa tgttaaaacg caaaacctga
     4261 ctcaatctat tgcccggtcg ttggctcgca tgcttcctcc ttctgcccca atccgacatg
     4321 actgagatgc gcttgatagc aatgaccaag atagtcgtct agattcatct ttagcgacgt
     4381 gacttagatt tgcagaggaa acggggtgac tgtagattat ggcagttgat atctctccct
     4441 gttatggagt gcggaggctg agtgctaatg ttgccccgca aagcagtgtc gttagaagta
     4501 tatatgcagt ggcggtgttc aatcaggatc agcccgtgct gatttctttg ttggggagaa
     4561 atgatattgg gcgggctcaa acttcaactc cgacggtcga atgccgggtt cttcgattcc
     4621 tgtttattgc gacgcacgct ctatttgctg gattacctgc attcaccgaa tcgcctaata
     4681 tacgtctaat cacctgtcaa tttaatcctg gtatactatc atccagggtg gtctaaagtc
     4741 ccatgagcca cgatgattaa ttttggtatg caccaagggg aggtggctag cgatccgagc
     4801 cgagctgcgt caccggttgg aaacacctgc gctaccctcc agtggtcgga attcgcgtat
     4861 cagcttgtcc gggtctacgt acacgcaaga cagatttcat cataccccct ggggattctt
     4921 agagactata tttcgtccga tgtccaccaa cataagcgag gctacctctt ggaggagcat
     4981 tcttgtatcc ccctcataga aagaccctgt gacgtcttaa caataggggc gacctagatc
     5041 cgttttcgta tttgacctta gtggtaaccg cagtacaacg ggcctgaaga tggtagtcga
     5101 gtgccaacac caaagaggct ctagcccagt gttcgggcgt cgttctacgg tacgtccatg
     5161 acggcagcag ctaccagatt ccggctgccc atcatggcat tatagtttat gaagagtgaa
     5221 tcgtagctaa ttcaaggcag taacgttcat agccgacagc tgacgctggg ttgtttaccc
     5281 gtaattcttc tccactctgc ttgagcggtg aggacccctt aatctgctcg acatccactt
     5341 tccatgtagg acgacaacgc ttggacaatc caatagttag atagctagtc ggacttctgg
     5401 cccgatctaa actcaaatcg cgggatcttc tgtcattcgt actgatgccg acccaatttc
     5461 aaaaacgtag gcgcacccac aggcgaggta cttatttatg tattgctctg tataagcctt
     5521 cgcctcgagt cctaagcagc gctttggctt cggtaaggcc tcatcacttc agaaacgcca
     5581 tccgtcgtaa gcgtgtgtgc ctccctagaa tcataggcca ctcgctactt cgtggggaca
     5641 ggccgcgaaa tgctagactc ataagaacat acggctccta atggctggga cgtcggttgt
     5701 cccgaagtgg cttgtaggct gtgtgtcaag tccagaacca ctccggacgg ccagcagacg
     5761 cggataatcc cggaaatagg taggattgac gaaccggata ctaggatgcc ctacggggcg
     5821 tagatctggc tccgcttctg acgagagtat cataattggc cttgttacat cctgtaagtc
     5881 taagctaagg cgccaggaga tggggtaggt gtgcttactg gtacgccgat acggacacga
     5941 gcatccaaca gtactatacc taatctctta tattcacatg ataacgcaca aactcgttat
     6001 tgtatagcac gaacagatat attgcacgtg attggaccga aaatgacttt ggacgccagc
     6061 ttaaaaggcg gttgcttcaa ttcgatgggt atctcatccg aatggacagt tggtaggccc
     6121 cttcagcgat tataccgcga cattctctgt cctagcaggt cagggaattt tccctaaggg
     6181 ctagtatcca cgcatcaatc gatgcggaca tggtcagatt gcatctgcgt aggaacagtt
     6241 tcgttgcggc aaggaaccgc ttgcggatca caccatattt ggatccccga gcaagtcttc
     6301 ttgggttaga ggcatacgta agaagcacgc cgtttgcgcg actttcgagc ctaacctggc
     6361 agggtttctt aattgtttga tgtactctat ctggaaattc gtgatatgcc agagcggccc
     6421 ggccccggat tagtcacacg atctgaactt atcccaggta gtgtttagcc gtgtagggat
     6481 attcgttttg tggttgtgct gcggaagtta ctacagcagg ggacccgagg tccgaacttt
     6541 aaacttttgt cagtcggtca gtatgtaagc ccaccatgga ggtcttcacc ttgctgctac
     6601 gtcattatcg tgccggatgt tccgtaactc agaactaccg agaagctatt attccgccaa
     6661 cccctcgtag agtctgaggg atcaagagac atttatcgac tcagtgcgta ataccgttcg
     6721 aagcgataga tcaatggtgc tccgtcagga cactatgact agaacaggta tgtcaaaccg
     6781 ataaggcaag catagtcaac gtgtcatgag tgacacttag gtcctatagt gcgacagaca
     6841 cgccaagacc atagacaatg aggtgacaac tagataagct cgatccttac ttgcgcacac
     6901 tcccccagct aaccaaacat cagatggacc tgtacactag tcttttacct aggagaacct
     6961 cgtttatctt attagcgggg agcaacaacg agctatagat gccactagcg tggccctgca
     7021 agttattcga gatgtcaggc aacctctgtt gcgagagctc gctgcacccc actcactcct
     7081 gcttcgtctt ccaaggtccc aagccgatcc atgctctcag gggtgggtcc gtgttgcata
     7141 aaagtctcca aaactcttaa ggagaggcgc gcacggatat acatcgcgtt taggttatta
     7201 tgtcccacgg catggccaaa tgagttcttg agtagcaaaa ggtgcacgag accccggtgc
     7261 ctaagatttt atacgcttca tcgggaatca ctgcccgaca attgctttcg tgttaataat
     7321 ttcgacgtaa cggtgctgtc taacatcgag actgcaatta ccccgccaga cctttgcact
     7381 tccacactaa tttggtcgat ctttgcttaa ccgggaacta tgtagtctat atgagaatat
     7441 tgagcataag gtgtcagcca gcctttatcc ttgaggcaga tcaggtctat tcgctcagag
     7501 taagatgcta acacccagta gatgacgacg tttaattagg gccgagagac caatgtcacg
     7561 aggatctgta ggtacgagta gaaaaggatt taaaatttga atagtccata gcagaatggc
     7621 cgcagggacg agtgtattaa tgtatgcgaa gcttacctcc tattagtttg ctcatctctg
     7681 ccgcccgtcc tcttcctatc actcggtctt atcacagagt cactatcgat cgtacaaagc
     7741 ttggtaccga cacataacgt cctgtgttct gcacgcaggc gctcgtctgg agatttacga
     7801 acgaactgaa cgatggtgag acactctaat tgagaacgcg gcttccatta acgaagatat
     7861 tacaatcatg ccaagtagtt tcaggaagta cagaacggag gtttacgctc cggcggcgag
     7921 cgatgagtat aacccgtcgc cttctacaac ctatgtctgc agctggttgg gcaaattgtg
     7981 gccgacgcgc tcccgacgaa tcagttgctg tgcccagaga gccatctaat gcttttcgat
     8041 actttgagga tggaatgcgt actccctcgg cacatgtatc gtatacacgt cagtgtgaac
     8101 tagcacctgg acacagatgt agccggtggg atgaacaatg cctactatgg cgcaattttt
     8161 ccgaaggacc attacttttt acgttaaacc ggccaaggct agtagtgatc tctaggaaat
     8221 ccgtacccaa ctttctggtg atacaggctg caacgtacta gagttcatga tcttgccctg
     8281 gtcaccaccg tgtcgaagtt gacaatatgc gcagtcaagg ttggtccggc ctcctgagcc
     8341 tggtggcaaa gtgcgctcat tctattaccg tcactggtcc ctatactgta aagtgtgtac
     8401 taagtaaacg gttgaatcct cccgcctgat ggcacaatgg caatcccatc ccgagtaatc
     8461 ttccggatcc tatgggaaat gtgggagcta ctactgctga ctgggaacac gtacggcgat
     8521 accgatgctt attctaatac gttccgctga ccctttcctg cgagttatga cgccggtatg
     8581 gatagttggc cccacacaat acttactagt tataatactc gcccggagaa tttgtttcgg
     8641 ccggcaattc acggccgcca tgggccctct tacccagaat ctcacttatg gtatttctaa
     8701 aagcctgccg gtctaggcag gttccctccg gtgtcttcgc gctaacggga ctgtcagacc
     8761 agcactgcgt tgatcgtttg tatgtgctac cgctgcctgg aatccatcgg ccttccagcc
     8821 cctgtctggg gttcggttag tgaacattca atacggtcat cagcgtttgg gcctcggaca
     8881 gcgaccgcaa tgcaaacaca aatccacatg gggctaactg tgtagacaca agactgatat
     8941 agcgttcccc taccgggtac tggatcatca agggcctttg ggatcgtcca cagctatgtg
     9001 agttgcaccg cgtaccggaa ctgctctaca ttccgtcttg agacggtggg cgatcgctct
     9061 agagctagcg aa
//
