LOCUS       sequence-249966-        9861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/
            N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T)  
            V16S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Abudayyeh OO, Gootenberg JS, Franklin B, Koob J, Kellner MJ, Ladha 
            A, Joung J, Kirchgatterer P, Cox DBT, Zhang F
  TITLE     A cytosine deaminase for programmable single-base RNA editing.
  JOURNAL   Science. 2019 Jul 11. pii: science.aax7063. doi: 
            10.1126/science.aax7063.
  PUBMED    31296651
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2019 Jul 11. pii: science.aax7063. doi: 10.1126/science.aax7063."
COMMENT     Sequence Label: pC0079 RESCUE-S
FEATURES             Location/Qualifiers
     source          1..9861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      917..926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5455..5472)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5461..5685
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5731..6159
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5818..5837)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6028..6049
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6168..6188)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6173..6502
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6353..6488
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6415..6434
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6569..7363
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6623..6642)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7233..7252
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7537..7658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7574..7593)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7628..7647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7707..7723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7707..7723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7720..7742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7731..7747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7755..7761,7762..7779,7780..7785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7800..7821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7938..7955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8109..8694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8189..8208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8865..9656,9657..9725))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9488..9507
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9726..9830)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatggagaa gcccctgctg cctaacgtgt ataccctgaa
      961 gcacaagttc ttttggggcg ccttcctgaa tatcgcccgg cacaacgcct ttatcaccat
     1021 ctgccacatc aatgagcagc tgggcctgaa aacccccagc aacgacgata agatcgtgga
     1081 cgtggtgtgc gagacatgga acaatatcct gaacaatgac cacgatctgc tgaagaagtc
     1141 ccagctgacc gagctgatcc tgaagcactt cccttttctg acagccatgt gctaccaccc
     1201 ccctaagaag gagggcaaga agaagggcca ccagaaggag cagcagaagg agaaggagag
     1261 cgaggcacag tcccaggcag aggccctgaa cccatccaag ctgatcgagg ccctggagat
     1321 cctggtgaat cagctgcact ctctggcaaa ctactatagc gcctataagc acaagaagcc
     1381 cgacgccgag aaggatatct tcaagcacct gtataaggcc ttcgacgcct ctctgagaat
     1441 ggtgaaggag gattataagg cccacttcac cgtgaatctg acaagggact ttgcccacct
     1501 gaaccgcaag ggcaagaaca agcaggacaa tcccgacttc aacagataca gattcgagaa
     1561 ggatggcttc tttaccgagt ctggcctgct gttctttaca aatctgtttc tggacaagag
     1621 ggatgcctat tggatgctga agaaggtgtc cggcttcaag gcctctcaca agcagcgcga
     1681 gaagatgacc acagaggtgt tttgcaggag ccgcatcctg ctgcccaagc tgaggctgga
     1741 gtcccgctac gaccacaacc agatgctgct ggatatgctg tctgagctga gcagatgtcc
     1801 taagctgctg tatgagaagc tgagcgagga gaataagaag cacttccagg tggaggccga
     1861 cggctttctg gatgagatcg aggaggagca gaacccattc aaggacaccc tgatccggca
     1921 ccaggataga ttcccctact ttgccctgag gtatctggac ctgaatgagt ccttcaagtc
     1981 tatccgcttt caggtggatc tgggcacata ccactattgt atctacgaca agaagatcgg
     2041 cgatgagcag gagaagaggc acctgacccg cacactgctg tccttcggcc ggctgcagga
     2101 ctttaccgag atcaacagac cccaggagtg gaaggccctg accaaggacc tggattacaa
     2161 ggagacatcc aatcagcctt tcatctctaa gaccacacca cactatcaca tcaccgacaa
     2221 caagatcggc tttcggctgg gcacaagcaa ggagctgtac ccctccctgg agatcaagga
     2281 tggcgccaat agaatcgcca agtaccctta taactccggc ttcgtggccc acgcctttat
     2341 ctctgtgcac gagctgctgc ctctgatgtt ctaccagcac ctgaccggca agagcgagga
     2401 cctgctgaag gagacagtgc ggcacatcca gagaatctat aaggacttcg aggaggagcg
     2461 gatcaatacc atcgaggatc tggagaaggc aaaccagggc agactgccac tgggagcctt
     2521 tcccaagcag atgctgggcc tgctgcagaa taagcagcct gatctgtccg agaaggccaa
     2581 gatcaagatc gagaagctga tcgccgagac aaagctgctg tctcacaggc tgaacacaaa
     2641 gctgaagagc tccccaaagc tgggcaagcg gagagagaag ctgatcaaga caggcgtgct
     2701 ggccgactgg ctggtgaagg atttcatgcg ctttcagccc gtggcctacg acgcccagaa
     2761 tcagcctatc aagtctagca aggccaactc caccgagttc tggtttatca ggcgcgccct
     2821 ggccctgtac ggaggagaga agaataggct ggagggctat ttcaagcaga ccaacctgat
     2881 cggcaacaca aatccacacc ccttcctgaa caagtttaat tggaaggcct gcaggaatct
     2941 ggtggatttc taccagcagt atctggagca gcgcgagaag tttctggagg ccatcaagaa
     3001 ccagccatgg gagccctacc agtattgcct gctgctgaag atccctaagg agaacaggaa
     3061 gaatctggtg aagggatggg agcagggagg aatctctctg ccacggggcc tgtttaccga
     3121 ggccatcaga gagacactgt ctgaggacct gatgctgagc aagccaatcc gcaaggagat
     3181 caagaagcac ggccgggtgg gcttcatcag cagagccatc accctgtact ttaaggagaa
     3241 gtatcaggat aagcaccaga gcttctacaa tctgtcctat aagctggagg caaaggcacc
     3301 actgctgaag cgggaggagc actacgagta ttggcagcag aacaagcctc agtctccaac
     3361 cgagagccag aggctggagc tgcacacaag cgaccgctgg aaggattacc tgctgtataa
     3421 gagatggcag cacctggaga agaagctgcg gctgtaccgg aatcaggacg tgatgctgtg
     3481 gctgatgacc ctggagctga caaagaacca cttcaaggag ctgaacctga attatcacca
     3541 gctgaagctg gagaatctgg ccgtgaacgt gcaggaggcc gatgccaagc tgaaccctct
     3601 gaatcagacc ctgcccatgg tgctgcctgt gaaggtgtac cctgccacag cctttggcga
     3661 ggtgcagtac cacaagaccc caatcaggac agtgtatatc cgcgaggagc acaccaaggc
     3721 cctgaagatg ggcaacttca aggccctggt gaaggaccgg agactgaatg gcctgttctc
     3781 ttttatcaag gaggagaacg atacacagaa gcacccaatc agccagctgc ggctgaggcg
     3841 cgagctggag atctaccagt ctctgagagt ggacgccttc aaggagacac tgagcctgga
     3901 ggagaagctg ctgaataagc acacatccct gtcctctctg gagaacgagt ttagggccct
     3961 gctggaggag tggaagaagg agtatgccgc cagctccatg gtgacagacg agcacatcgc
     4021 cttcatcgcc agcgtggcca atgccttctg cgccaaccag taccccttct acaaggaggc
     4081 cctgcacgcc cctatcccac tgttcaccgt ggcccagccc accacagagg agaaggatgg
     4141 cctgggaatc gcagaggccc tgctgaaggt gctgagggag tactgtgaga tcgtgaagtc
     4201 ccagatcgga tccagcctcc aaaaaaagtt ggaggaatta gaactaggat cccagctgca
     4261 tttaccgcag gttttagctg acgctgtctc acgcctggtc ataggtaagt ttggtgacct
     4321 gaccgacaac ttctcctccc ctcacgctcg cagaataggt ctggctggag tcgtcatgac
     4381 aacaggcaca gatgttaaag atgccaaggt gatatgtgtt tctacaggag caaaatgtat
     4441 taatggtgaa tacctaagtg atcgtggcct tgcattaaat gactgccatg cagaaatagt
     4501 atctcggaga tccttgctca gatttcttta tacacaactt gagctttact taaataacga
     4561 ggatgatcaa aaaagatcca tctttcagaa atcagagcga ggggggttta ggctgaagga
     4621 gaatatacag tttcatctgt acatcagcac ctctccctgt ggagatgcca gaatcttctc
     4681 accacatgag gcaatcctgg aagaaccagc agatagacac ccaaatcgta aagcaagagg
     4741 acagctacgg accaaaatag aggctggtca ggggacgatt ccagtgcgca acaatgcgag
     4801 catccaaacg tgggacgggg tgctgcaagg ggagcggctg ctcaccatgt cctgcagtga
     4861 caagattgca cgctggaacg tggtgggcat ccagggatca ctgctcagca ttttcgtgga
     4921 gcccatttac ttctcgagca tcatcctggg cagcctttac cacggggacc acctttccag
     4981 ggccatgtac cagcggatct ccaacataga ggacctgcca cctctctaca ccctcaacaa
     5041 gcctttgctc acaggcatca gcaatgcaga agcacggcag ccagggaagg cccccatatt
     5101 cagtgtcaac tggacggtag gcgactccgc tattgaggtc atcaacgcca cgactgggaa
     5161 gggagagctg ggccgcgcgt cccgcctgtg taagcacgcg ttgtactgtc gctggatgcg
     5221 tgtgcacggc aaggttccct cccacttact acgctccaag attaccaagc ccaacgtgta
     5281 ccatgagaca aagctggcgg caaaggagta ccaggccgcc aaggcgcgtc tgttcacagc
     5341 cttcatcaag gcggggctgg gggcctgggt ggagaagccc accgagcagg accagttctc
     5401 actcacgtaa gcggccgctc gagtctagag ggcccattta aacccgctga tcagcctcga
     5461 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     5521 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     5581 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     5641 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5701 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5761 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5821 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5881 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5941 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6001 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6061 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6121 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     6181 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6241 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6301 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6361 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6421 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     6481 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     6541 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     6601 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     6661 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     6721 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     6781 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6841 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6901 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6961 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     7021 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     7081 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     7141 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     7201 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     7261 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     7321 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     7381 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     7441 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     7501 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     7561 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7621 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     7681 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7741 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7801 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7861 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7921 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7981 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8041 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8101 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8161 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8221 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8281 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8341 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8401 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8461 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8521 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8581 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8641 agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8701 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8761 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8821 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8881 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8941 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9001 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9061 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9121 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9181 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9241 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9301 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9361 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9421 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9481 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9541 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9601 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9661 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9721 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9781 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9841 cgaaaagtgc cacctgacgt c
//
