LOCUS       sequence-249970-        6066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre with chimeric/beta-globin intron within the Cre coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Kaczmarczyk SJ, Green JE
  TITLE     A single vector containing modified cre recombinase and LOX 
            recombination sequences for inducible tissue-specific amplification 
            of gene expression.
  JOURNAL   Nucleic Acids Res 2001 Jun 15;29(12):E56-6.
  PUBMED    11410679
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "2001-06-15"; volume: "29(12)"; pages: "E56-6"
COMMENT     Sequence Label: p210 pCMV-CREM
FEATURES             Location/Qualifiers
     source          1..6066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          931..1063
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(3365..3381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3365..3381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3378..3400)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3389..3405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3413..3419,3420..3437,3438..3443))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3458..3479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3596..3613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3767..4355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3847..3866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4526..5317,5318..5386))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5149..5168
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5387..5491)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5559..5577
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5615..5637)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5737..5756
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5950..5972
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5964..5981
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5965..5981
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctaga
      661 ggatcccctc gagcggccgc ctaggccatg gcgaatttac tgacggtacc agaattccaa
      721 aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac
      781 atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc
      841 cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa
      901 gatgttcgcg attatcttct atatctgcag gtaagtatca aggttacaag acaggtttaa
      961 ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca
     1021 cctattggtc ttactgacat ccactttgcc tttctctcca caggcgcgcg gtctggcagt
     1081 aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc
     1141 acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt
     1201 tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt
     1261 tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg
     1321 gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat
     1381 ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag
     1441 caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat
     1501 ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa
     1561 tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt
     1621 tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc
     1681 ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc
     1741 aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtca tgaactatat
     1801 ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattagcc
     1861 attaacgcgc gtaaatgatt gctataatta tttgatattt atggtgacat atgagaaagg
     1921 atttcaacat cgacggaaaa tatgtagtgc tgtctgtaag cactaatatt cagtcgccag
     1981 ccgtcattgt cactgtaaag ctgagcgata gaatgcctga tattgactca atatccgttg
     2041 cgtttcctgt caaaagtatg cgtagtgctg aacatttcgt gatgaatgcc accgaggaag
     2101 aagcacggcg cggttttgct aaagtgatgt ctgagtttgg cgaactcttg ggtaaggttg
     2161 gaattgtcga gggatctcgg aatggacccc aactgctcct gctccaccgg taagactccc
     2221 gatccttggt ctttagaata ccaagttggg accgcagagc ggaatccccg agttgtagag
     2281 gcttggcggg aataggcacc tttagttggc gattcattcc ggttctttct agaatccgct
     2341 cttgcaaaag ccttcattag ttacgagtat tgtcgaacgg gtcctttggc ggggttgggg
     2401 ctaggattta gacgcgcaaa tgtccggttc ctgatcaccc agttagtggg gacatctggg
     2461 ttgagtccca ggcattacta aacttactgt gaattgcttg aattaagaaa gaggtgaagg
     2521 acctttatgt cttgggactc aaagacataa tccctgactt aacctgtgag gagaaaagtg
     2581 gggctaggct ccctgcagct ccgaggagga cttagtgaac tgagccggga ctcgtggtgt
     2641 tggccactgc tgtaatgctg cctccctcat gctgtcttct ttctcctccc caggcggctc
     2701 ctgcacttgc accagctcct gcgcctgcaa gaactgcaag tgcacctcct gcaagaagag
     2761 tgagttggga caccttgggt ggcggctaag gctaggggcg gggaactcct acaaaactgg
     2821 ctctgagaaa tgtcctttgc ttcccggagg ccattgtatt gtctcgggga cagaactata
     2881 cagagaacta tttaaaaaaa ccgaggtctt ctctgttggg gacaggaagc agaggtcttc
     2941 agccaggctg acctcttcct cctcctttct aggctgctgc tcctgctgtc ccgtgggctg
     3001 ctccaaatgt gcccagggct gtgtctgcaa aggcgccgcg gacaagtgca cgtgctgtgc
     3061 ctgatgtgac gaacagcgct gccaccacgt gtaaatagta tcggaccaac ccagcgtctt
     3121 cctatacagt tccaccctgt ttactaaacc cccgttttct accgagtacg tgaataataa
     3181 aagcctgttt gagtctaact ctggttttct tggtgtggtt tggcaataag aaactggggt
     3241 gacttgatag tctggggatc tggttttgga ccccctcgtg cctttacctc ccgcctctgg
     3301 ccctcacaga ggggtaatgt ctttgggtaa agccaagcta tcccataagc taattcgtaa
     3361 tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     3421 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3481 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3541 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3601 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3661 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3721 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3781 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3841 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3901 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3961 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4021 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4081 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4141 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4201 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4261 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4321 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4381 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4441 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4501 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4561 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4621 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4681 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4741 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4801 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4861 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4921 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4981 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5041 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5101 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5161 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5221 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5281 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5341 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5401 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5461 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     5521 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     5581 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     5641 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     5701 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     5761 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     5821 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     5881 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     5941 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt ggcccattgc
     6001 atacgttgta tccatatcat aatatgtaca tttatattgg ctcatgtcca acattaccgc
     6061 catgtt
//
