LOCUS       sequence-249983-        2620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to 
            be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK7_013
FEATURES             Location/Qualifiers
     source          1..2620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      77..665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     566..585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    947..1476
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     terminator      complement(1751..1785)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1856..2515)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2430..2449
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg tctcagagtg
      781 attggtgaca gaaaagcccc atccttaggc ctcctccttc ctagtctcct gatattgggt
      841 ctaaccccca cctcctgtta ggcagattcc ttatctggtg acacaccccc atttcctgga
      901 gccatctctc tccttgccag aacctctaag gtttgcttac gatggagcca gagaggatcc
      961 tgggagggag agcttggcag ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt
     1021 ctcagtggcc accctgcgct accctctccc agaacctgag ctgctctgac gcggccgtct
     1081 ggtgcgtttc actgatcctg gtgctgcagc ttccttacac ttcccaagag gagaagcagt
     1141 ttggaaaaac aaaatcagaa taagttggtc ctgagttcta actttggctc ttcacctttc
     1201 tagtccccaa tttatattgt tcctccgtgc gtcagtttta cctgtgagat aaggccagta
     1261 gccagccccg tcctggcagg gctgtggtga ggaggggggt gtccgtgtgg aaaactccct
     1321 ttgtgagaat ggtgcgtcct aggtgttcac caggtcgtgg ccgcctctac tccctttctc
     1381 tttctccatc cttctttcct taaagagtcc ccagtgctat ctgggacata ttcctccgcc
     1441 cagagcaggg tcccgcttcc ctaaggccct gctctgggct tctgggtttg agtccttggc
     1501 aagcccagga gaggcgctca ggcttccctg tcccccttcc tcgtccacca tctcatgccc
     1561 ctggctctcc tgccccttcc ctacaggggt tcctggctct gctctaccta ctgcattaaa
     1621 aaaaaatttt tttttttcta agacaaggtc tgactctgtc tcccaggctg gagtgcagtg
     1681 gtgcaatcac gctcatagcc tcaacctccc gggctccagt gggcctccca cctccgatga
     1741 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1801 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     1861 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     1921 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     1981 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     2041 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
     2101 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     2161 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     2221 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     2281 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     2341 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     2401 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     2461 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     2521 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     2581 cattatggtg aaagttggaa cctcttacgt gcccgatcaa
//
