LOCUS       sequence-249984-        2373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part 
            to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK7_014
FEATURES             Location/Qualifiers
     source          1..2373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      77..665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     566..585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             846..1079
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1151..1272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1188..1207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1242..1261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1312..1447
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1467..1486)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1468..1486)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(1504..1538)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1609..2268)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2183..2202
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg tctcagagtc
      781 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
      841 gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg
      901 atctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      961 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     1021 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     1081 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     1141 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     1201 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     1261 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     1321 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     1381 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     1441 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattaccga tgagaccaga
     1501 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     1561 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
     1621 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     1681 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     1741 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     1801 aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc
     1861 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     1921 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     1981 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     2041 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     2101 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     2161 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     2221 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     2281 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     2341 gtgaaagttg gaacctctta cgtgcccgat caa
//
