LOCUS       sequence-249991-        6188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C
  TITLE     Transcription elongation factor ELL2 directs immunoglobulin 
            secretion in plasma cells by stimulating altered RNA processing.
  JOURNAL   Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009
            Sep 13.
  PUBMED    19749764
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1786"; 
            journalName: "Nat Immunol"; date: "2009-10"; volume: "10"; issue: 
            "10"; pages: "1102-9"
COMMENT     Sequence Label: Met138ILeu, Met186ILeu ELL2
FEATURES             Location/Qualifiers
     source          1..6188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(162..173)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(2899..2917)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2938..2954)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2938..2954)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2951..2973)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2962..2978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2986..2992,2993..3010,3011..3016))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3031..3052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3169..3186)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3340..3928)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3420..3439)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4099..4890,4891..4959))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4722..4741
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4960..5064)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5090..5545)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5227..5248)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5439..5458
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5672..5694
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5686..5703
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5687..5703
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5710..5729
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5710..5728
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5761..5794)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atggcggcgg gggggacagg gggcctgcgg gaggagcagc gctatgggct gtcgtgcgga
       61 cggctggggc aggacaacat caccgtactg catgtgaagc tcaccgagac ggcgatccgg
      121 gcgctcgaga cttaccagag ccacaagaat ttaattcctt ttcgaccttc aatccagttc
      181 caaggactcc acgggcttgt caaaattccc aaaaatgatc ccctcaatga agttcataac
      241 tttaactttt atttgtcaaa tgtgggcaaa gacaaccctc agggcagctt tgactgcatc
      301 cagcaaacat tctccagctc tggagcctcc cagctcaatt gcctgggatt tatacaagat
      361 aaaattacag tgtgtgcaac aaacgactcg tatcagatga cacgagaaag aatcacccag
      421 gcagaggagg aatcccgcaa ccgaagcaca aaagttatca aacccggtgg accatatgta
      481 gggaaaagag tgcaaattcg gaaagcacct caagctgttt cagatacagt tcctgagagg
      541 aaaaggtcaa cccccatcaa ccctgcaaat acaattcgaa agacacatag cagcagcacc
      601 atctctcaga ggccatacag ggacagggtg attcacttac tggccctgaa ggcctacaag
      661 aaaccggagc tacttgctag actccagaaa gatggtgtca atcaaaaaga caagaactcc
      721 ctgggagcaa ttctgcaaca ggtagccaat ctgaattcta aggacctctc atatacctta
      781 aaggattatg tttttaaaga gcttcaaaga gactggcctg gatacagtga aatagacaga
      841 cggtcattgg agtcagtgct ctctagaaaa ctaaatccgt ctcagaatgc tacaggcacc
      901 agccgttcag aatctcctgt atgttctagt agagatgctg tatcttctcc tcagaaacgg
      961 cttttggatt cagagtttat tgatccttta atgaataaaa aagcccgaat atctcacctg
     1021 acgaacagag taccaccaac actaaatggt catttgaatc ccaccagtga aaaatcggct
     1081 gcaggcctcc cactgccccc tgcggctgct gccatcccca cccctccacc gctgccttca
     1141 acctatctgc ccatctcaca tcctcctcag attgtaaatt ctaactccaa ctcccctagc
     1201 actccagaag gccgggggac tcaagaccta cctgttgaca gttttagtca aaacgatagt
     1261 atctatgagg accagcaaga caaatatacc tctaggactt ctctggaaac cttaccccct
     1321 ggttccgttc tactaaagtg tccaaagcct atggaagaaa accattcaat gtctcacaaa
     1381 aagtccaaaa agaagtctaa aaaacataag gaaaaggacc aaataaaaaa gcacgacatt
     1441 gagactattg aggaaaagga ggaagatctt aagagagaag aggaaattgc caagctaaat
     1501 aactccagtc caaattccag tggaggagtt aaagaggatt gcactgcctc catggaacct
     1561 tcagcaattg aactcccaga ttatttgata aaatatatcg ctatcgtctc ctatgagcaa
     1621 cgccagaatt ataaggatga cttcaatgca gagtatgatg agtacagagc tttgcatgcc
     1681 aggatggaga ctgtagctag aagatttatc aaactagatg cacaaagaaa gcgcctttct
     1741 ccaggctcaa aagagtatca gaatgttcat gaagaagtct tacaagaata tcagaagata
     1801 aagcagtcta gtcccaatta ccatgaagaa aaatacagat gtgaatatct tcataacaag
     1861 ctggctcaca tcaaaaggct aataggtgaa tttgaccaac agcaagcaga gtcatggtcc
     1921 tagaactctg cttggaccag aagatgtgaa taaacttaag ctaacccccc attgcaatgg
     1981 ttgccttaag gtgtttgcta gttgtgtaca tagtgtggtt aatcattagc tacactgctt
     2041 cccacttgat tagagcaatg ggaagcatac tgtggcctac cagcatctgg aagtgtgtgc
     2101 tcgatctgta tgtgtgcaga ggtggtgtgg atgtgagcgt gcatgaagga aaaaaagctg
     2161 ctactcctag taggccaaac gctcaggtta aacaactgac gagtgttact gtagggtgtt
     2221 tttttgtttt tttgtttttt ttcttctatc aaattgctac ttttgttgtg gaagacaaaa
     2281 gcatttccat ttcaacgagt ttgtcagctt tattaatgtt gggcaaaaat tgatatgtca
     2341 tgaaaatgaa acagatctat agttttggga caaaattata aaatgaaatg tgtaggtaac
     2401 ctatttatat actgctataa agtatttttt gaagagagat atgcaaagaa gctattacct
     2461 acataagagg tatatttaaa gatttttttt tttttcatcc tggtgccagg aatataaaaa
     2521 agagtggata tatttaacca taacatactg tgattcatca aacagcacaa actttcattt
     2581 catggagttt atctgttgac attgatttaa actgtcactt gttttatcat gtgggaacat
     2641 aagttatgtg gtcaaaaata taaggatttt gaattaatgt tgattcaagt tgtattgtct
     2701 tttcaaagtg ctgccagttg aaaagggaag cattatgttt acaaatctgt tttgaaatgt
     2761 ttgccaaaat tttggtagtg tctttaataa agatgtttgt ctccagcatc cagaaaaaaa
     2821 aaaaaaaaac tctccagcgc tggatccggc cataagggcc tgatccttcg agggggggcc
     2881 cggtaccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc
     2941 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3001 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3061 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3121 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3181 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3241 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3301 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3361 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3421 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3481 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3541 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3601 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3661 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3721 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3781 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3841 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3901 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3961 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4021 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4081 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4141 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4201 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4261 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4321 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4381 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4441 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4501 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4561 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4621 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4681 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4741 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4801 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4861 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4921 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4981 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5041 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     5101 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     5161 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     5221 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     5281 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     5341 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     5401 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     5461 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     5521 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     5581 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     5641 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     5701 agtgaattgt aatacgactc actatagggc gaattggagc tccaccgcgg tggcggccgc
     5761 ataacttcgt atagcataca ttatacgaag ttatggatca ggccaaatcg gccgagctcg
     5821 aattcgtcga gagcgggata agttggcgcg tcacctagac gtcagcctct tacctctcgg
     5881 acgtcccgtc ccaggagcaa aactctagtg actttgggcc cgcccccgtg agagtggagg
     5941 gcggccgatg agggcggttc tgaagggaac aagaccaatg agacgagccc ggggcggggc
     6001 gcggggccgg agggtggggc gggtggcggc ggtggcagtc cgaggtctgg ctccgcgcag
     6061 tatatgacag tacgtcagca gcgggatggc cctagcagtg gcggcggctg cagaagccca
     6121 agcagccgcg gccgcagtgg aggctagagc cggagcggcg gcggcggcgg caccccgggg
     6181 agtttaag
//
