LOCUS       sequence-25000-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Bandyopadhyay A, Kubilus JK, Crochiere ML, Linsenmayer TF, Tabin CJ
  TITLE     Identification of unique molecular subdomains in the perichondrium 
            and periosteum and their role in regulating gene expression in the 
            underlying chondrocytes.
  JOURNAL   Dev Biol. 2008 Sep 1;321(1):162-74. doi: 
            10.1016/j.ydbio.2008.06.012. Epub 2008 Jun 16.
  PUBMED    18602913
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2008.06.012"; journalName: "Dev Biol"; date: 
            "2008-09-1- 1"; volume: "321"; issue: "1"; pages: "162-74"
COMMENT     Sequence Label: pBS DKK3
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..41
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     25..41
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(666..685)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(667..685)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(695..712)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(695..711)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(704..726)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gggggctccc gcggtggcgg ccgctctaga actagtggat cccccgggct gcaggaattc
       61 ggcacgaggc ctcgtgccgc cacagntact acatctgtgt gtgaactgtc ctccaatgaa
      121 accaggaaaa atgaaaaaga agatcccttg aacatggatg agatgccatt tatcagttta
      181 atacccagag atattctttc tgattacgaa gaaagcagcg tcattcagga agtgcgtaaa
      241 gaattagaaa gcctggagga ccaagcaggt gtgaagtctg agcatgaccc ggctcatgac
      301 ctatttctgg gagatgaaat atgaagttca aacaccagtt tagttagtcc tagaaattgt
      361 tgtctagtgt cttgcttaca tacaccctta acagatactg ctggatagaa gtgcaataaa
      421 catcttcatt gagcatccgt tttcgtgcac caaacctgca tgttcaaatt catgttgaat
      481 tcactcaatc tttggaccaa actttccatc aaagacaaat gagaaaggca tcagtgtttc
      541 ctttggatga atactttcct ttgtacagca gaaataaacg tatcagtact tgtactcatt
      601 aaaaaaacac acggagcata aaaaaaaaaa aaaaaaactc gagggggggc ccggtaccca
      661 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
      721 actgggaacc gntcntactc cccnt
//
