LOCUS       sequence-250024-        3785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IgH V-region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Shell SA, Martincic K, Tran J, Milcarek C
  TITLE     Increased phosphorylation of the carboxyl-terminal domain of RNA 
            polymerase II and loading of polyadenylation and cotranscriptional 
            factors contribute to regulation of the ig heavy chain mRNA in 
            plasma cells.
  JOURNAL   J Immunol. 2007 Dec 1;179(11):7663-73.
  PUBMED    18025212
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Immunol."; date: "2007-12-1"; volume: "179(11)"; pages: "7663-73"
COMMENT     Sequence Label: IgH P+ VA20
FEATURES             Location/Qualifiers
     source          1..3785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(462..481)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(463..481)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(562..579)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(733..1321)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(813..832)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1492..2283,2284..2352))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2115..2134
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2353..2457)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2525..2543
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2581..2603)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2703..2722
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2932..2951
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3241..3259
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3241..3258
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atggatgaca ctgacaccaa ccttgccttt ctctccacag ttgtccactc cccggtccaa
       61 ttgcaacagt ccgggcctga ccttgtgaaa cctgggatgt ccgtgaaatt gtcctgtaag
      121 actttgggtt acaatttctc cgacaagtgg attcactgga ttaaacagaa gcctggccga
      181 ggccttgaat gggttggaag gattgatcct tctaacggtg atactgacta taatgtggac
      241 ttcaagaccc cggccacatt aactgttgac agaccctcca acacagccta cttagaactc
      301 agtaacctga catctgggga ctctgcggtc tattattgtt caatatcggg tgattattcc
      361 gcctgcgact attggaagcc gaattctgca gatatccatc acactggcgg ccgctcgagc
      421 atgcatctag agtcgacctg caggcatgca agcttgggtc tccctatagt gagtcgtatt
      481 aatttcgata agccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      541 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      601 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      661 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      721 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      781 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      841 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      901 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      961 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1021 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1081 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1141 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1201 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1261 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1321 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1381 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1441 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1501 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1561 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1621 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1681 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1741 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1801 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1861 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1921 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1981 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2041 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2101 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2161 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2221 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2281 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2341 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2401 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2461 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     2521 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     2581 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     2641 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     2701 tgcggcatca gagcagattg tactgagagt gcaccattcg acgctctccc ttatgcgact
     2761 cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga
     2821 atggtgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     2881 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     2941 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
     3001 ggcgtagagg atctggctag cgatgaccct gctgattggt tcgctgacca tttccgggtg
     3061 cgggacggcg ttaccagaaa ctcagaaggt tcgtccaacc aaaccgactc tgacggcagt
     3121 ttacgagaga gatgataggg tctgcttcag taagccagat gctacacaat taggcttgta
     3181 catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     3241 atttaggtga cactatagaa tacacggaat tcgagctcgg taccgagctc ggatccacta
     3301 gtaacggccg ccagtgtgct ggaattcggc ttactatagg gcacgcgtgg tcgacggccc
     3361 gggctggtct gtgtcacagt ggggccactg tctcaagctg caaatctttt tagtgcacag
     3421 gctctaatgt tacatccata gcctcaacac aaggttcagg gatgaagtat gggatgaatt
     3481 tccacagaca agatgaggac ttgggcttca gaatcctgat tcctgaccca gatgtccctt
     3541 cttctccagc aggagtaggt gcttatctaa tatgtatcct gctcatgaat atgcaaatcc
     3601 tgtgggtcta cagtggtaaa tatagggttg tctgcacgaa acagaaaccc tgagatcgct
     3661 gctctcttta cagttgctga atacacaaga cctcaccatg gcatcgaact tcatcatggt
     3721 cttcttgaca acaacagctc caggtgaggg gctcacacta acagccttga ggtctggcca
     3781 cacac
//
