LOCUS       sequence-250032-       10021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Progesterone inducible expression of KeyA-BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Langan RA, Boyken SE, Ng AH, Samson JA, Dods G, Westbrook AM, Nguyen
            TH, Lajoie MJ, Chen Z, Berger S, Mulligan VK, Dueber JE, Novak WRP, 
            El-Samad H, Baker D
  TITLE     De novo design of bioactive protein switches.
  JOURNAL   Nature. 2019 Jul 24. pii: 10.1038/s41586-019-1432-8. doi: 
            10.1038/s41586-019-1432-8.
  PUBMED    31341284
REFERENCE   2  (bases 1 to 10021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2019 Jul 24. pii: 10.1038/s41586-019-1432-8. doi: 
            10.1038/s41586-019-1432-8."
COMMENT     Sequence Label: pAN1578
FEATURES             Location/Qualifiers
     source          1..10021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3750..3773
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     promoter        5207..5614
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             5615..6709
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(7739..8554)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7739..8554)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8462..8481
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8546..8565
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8779..9367
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9268..9287
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgaaggc aagcccagaa aaatatcgca agcacctttg
       61 gtcttacagt gccaactttt ggcctgccga cgttaagagt acaaagctga tggcaatgta
      121 cgacaagata acagagtctc aaaagaagtg aaacaatttt tcttcaccac attttccatt
      181 gttccttccc cccataacta taaacgtatt tatgtatata tatttgcgtg taagtgtgtg
      241 tactataggg caccgtaaag taataatgct taattagtta ctactatgac catataagag
      301 gtcatactgt atgaagccac aaagcagata gatcaatcat gtttaacgaa aactgttaat
      361 cgaagattat ttcttttttt ttttctcttt cctttttaca aagaaaattt tttttgcgct
      421 ttttgccatc accatcgcaa gttctgggac aattgttctc tttcgctcca gttccaagga
      481 aagaggtttc tgttttactt aatagaaagt gtcatcttgt attttatatc tcttctttct
      541 tgtgtaaaat tctttagttt tgattttgta tttttaggac agtgagctac gaagtaacat
      601 ttttacttaa taaccgtttg aagcatagag caggccctgg tatcaccacc taatatctgg
      661 ctttttattc aataaaaact caaaaaaaaa aatccaaaaa aaactaaaaa accaataaaa
      721 ataaaagatc tatgggtacc cgcccatatg cttgccctgt cgagtcctgc gatcgccgct
      781 tttctcgctc ggatgagctt acccgccata tccgcatcca taccggtcag aagcccttcc
      841 agtgtcgaat ctgcatgcgt aacttcagtc gtagtgacca ccttaccacc cacatccgca
      901 cccacacagg cgagaagcct tttgcctgtg acatttgtgg gaggaagttt gccaggagtg
      961 atgaacgcaa gaggcatacc aaaatccata caggtagatc cggtgacggt gctggtttaa
     1021 agttcaataa agtcagagtt gtgagagcac tggatgctgt tgctctccca cagccattgg
     1081 gcgttccaaa tgaaagccaa gccctaagcc agagattcac tttttcacca ggtcaagaca
     1141 tacagttgat tccaccactg atcaacctgt taatgagcat tgaaccagat gtgatctatg
     1201 caggacatga caacacaaaa cctgacacct ccagttcttt gctgacaagt cttaatcaac
     1261 taggcgagag gcaacttctt tcagtagtca agtggtctaa atcattgcca ggttttcgaa
     1321 acttacatat tgatgaccag ataactctca ttcagtattc ttggatgagc ttaatggtgt
     1381 ttggtctagg atggagatcc tacaaacatg tcagtgggca gatgctgtat tttgcacctg
     1441 atctaatact aaatgaacag cggatgaaag aatcatcatt ctattcatta tgccttacca
     1501 tgtggcagat cccacaggag tttgtcaagc ttcaagttag ccaagaagag ttcctctgta
     1561 tgaaagtatt gttacttctt aatacaattc ctttggaagg gctacgaagt caaacccagt
     1621 ttgaggagat gaggtcaagc tacattagag agctcatcaa ggcaattggt ttgaggcaaa
     1681 aaggagttgt gtcgagctca cagcgtttct atcaacttac aaaacttctt gataactcgc
     1741 atgatcttgt caaacagctt catctgtact gcttgaatac atttatccag tcccgggcac
     1801 tgagtgttga atttccagaa atgatgtctg aagttattgc tgcacaatta cccaagatat
     1861 tggcagggat ggtgaaaccc cttctctttc ataaaaaggc ggctgcaggt gacggtgctg
     1921 gtttaattaa catgacggtc gaccatgatt tcaatagcga agatatttta ttccccatag
     1981 aaagcatgag tagtatacaa tacgtggaga ataataaccc aaataatatt aacaacgatg
     2041 ttatcccgta ttctctagat atcaaaaaca ctgtcttaga tagtgcggat ctcaatgaca
     2101 ttcaaaatca agaaacttca ctgaatttgg ggcttcctcc actatctttc gactctccac
     2161 tgcccgtaac ggaaacgata ccatccacta ccgataacag cttgcatttg aaagctgata
     2221 gcaacaaaaa tcgcgatgca agaactattg aaaatgatag tgaaattaag agtactaata
     2281 atgctagtgg ctctggggca aatcaataca caactcttac ttcaccttat cctatgaacg
     2341 acattttgta caacatgaac aatccgttac aatcaccgtc accttcatcg gtacctcaaa
     2401 atccgactat aaatcctccc ataaatacag caagtaacga aactaattta tcgcctcaaa
     2461 cttcaaatgg taatgaaact cttatatctc ctcgagccca acaacatacg tccattaaag
     2521 ataatcgtct gtccttacct aatggtgcta attcgaatct tttcattgac actaacccaa
     2581 acaatttgaa cgaaaaacta agaaatcaat tgaactcaga tacaaattca tattctaact
     2641 ccatttctaa ttcaaactcc aattctacgg gtaatttaaa ttccagttat tttaattcac
     2701 tgaacataga ctccatgcta gatgattacg tttctagtga tctcttattg aatgatgatg
     2761 atgatgacac taatttatca cgccgaagat ttagcgacgt tataacaaac caagagctcg
     2821 gatcctaact cgagataaag caatcttgat gaggataatg attttttttt gaatatacat
     2881 aaatactacc gtttttctgc tagattttgt gatgacgtaa ataagtacat attacttttt
     2941 aagccaagac aagattaagc attaacttta cccttttctt tctaagtttc aatattagtt
     3001 atcactgttt aaaagttatg gcgagaacgt cggcggttaa aatatattac cctgaacggc
     3061 tgccaaacca gatgtcaaca cagctacaac gttatattga attttcaaaa attcttactt
     3121 tttttttgga tggacgcaaa gaagtttaat aatcatatta catggcatta ccaccatata
     3181 catatccata tacatatcca tatctaatct tacttatatg ttgtggaaat gtaaagagcc
     3241 ccattatctt agcctaaaaa aaccttctct ttggaacttt cagtaatacg cttaactgct
     3301 cattgctata ttgaagtgtg gccgcgtggg cgtgcgtggg cgggcgtggg cgtgcgtggg
     3361 cgggcgtggg cgtgcgtggg cgtctagacc gtgcgtcctc gtcttcaccg gtcgcgttcc
     3421 tgaaacgcag atgtgcctaa tgccgcactg ctccgaacaa taaagattct acaatactag
     3481 cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatta
     3541 acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg
     3601 ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa atggaaaagc
     3661 tgcataacca ctttaactaa tactttcaac attttcagtt tgtattactt cttattcaaa
     3721 tgtcataaaa gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga
     3781 aaaaactata agatctatgg aggctagaaa agccatcgct agagtaaaga gagaatcaaa
     3841 gagaatcgta gaagatgctg agaggttaat aagagaagca gccgccgcat ctgagaaaat
     3901 ttcaagggag gccgaaaggt tgattaggga ggcagctgcg gcttcagaaa agataagtag
     3961 ggagggttct gtttcaaagg gtgaagaatt gatcaaagaa aacatgcata tgaaattata
     4021 tatggaaggt acagttgaca accatcattt taagtgtaca tctgaaggag aaggtaagcc
     4081 ttacgaggga actcaaacta tgagaataaa agtcgtagaa ggtggaccat tgccttttgc
     4141 ttttgatatc ttagcaacat ctttcttata tggttctaaa acctttatta accatactca
     4201 aggtatccct gatttcttca aacaatcttt ccctgaagga tttacttggg agagggttac
     4261 aacatatgag gatggtggtg ttttgacagc aacccaggat acatcattgc aggatggatg
     4321 tttgatttat aatgtaaaga ttagaggagt aaattttact tctaacggtc ctgtaatgca
     4381 aaagaagact ttaggttggg aagctttcac cgaaacatta tatccagcag acggaggttt
     4441 agagggaaga aacgatatgg ctttgaagtt agtcggtggt tcacacttaa tagctaacgc
     4501 caagactact tacaggtcta agaaaccagc aaagaatttg aaaatgccag gagtttatta
     4561 tgttgactac agattggaaa ggatcaagga ggccaataac gagacatatg tagagcagca
     4621 tgaagtcgcc gtcgccagat attgcgactt accttctaag ttgggtcata aattgaatgg
     4681 atcctaactc gagagctttt gattaagcct tctagtccaa aaaacacgtt tttttgtcat
     4741 ttatttcatt ttcttagaat agtttagttt attcatttta tagtcacgaa tgttttatga
     4801 ttctatatag ggttgcaaac aagcattttt cattttatgt taaaacaatt tcaggtttac
     4861 cttttattct gcttgtggtg acgcgtgtat ccgcccgctc ttttggtcac ccatgtatgc
     4921 tgagcagtta cagagatgtt acgaaccact agtgcactgc agtacatcga ggagaacttc
     4981 tagtatatct acatacctaa tattattgcc ttattaaaaa tggaatccca acaattacat
     5041 caaaatccac attctcttca aaatcaattg tcctgtactt ccttgttcat gtgtgttcaa
     5101 aaacgttata tttataggat aattatactc tatttctcaa caagtaattg gttgtttggc
     5161 cgagcggtct aaggcgcctg attcaagaaa tatcttgacc gcagttaact gtgggaatac
     5221 tcaggtatcg taagatgcaa gagttcgaat ctcttagcaa ccattatttt tttcctcaac
     5281 ataacgagaa cacacagggg cgctatcgca cagaatcaaa ttcgatgact ggaaattttt
     5341 tgttaatttc agaggtcgcc tgacgcatat acctttttca actgaaaaat tgggagaaaa
     5401 aggaaaggtg agagcgccgg aaccggcttt tcatatagaa tagagaagcg ttcatgacta
     5461 aatgcttgca tcacaatact tgaagttgac aatattattt aaggacctat tgttttttcc
     5521 aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca tttcagcaat
     5581 atatatatat atatttcaag gatataccat tctaatgtct gcccctaaga agatcgtcgt
     5641 tttgccaggt gaccacgttg gtcaagaaat cacagccgaa gccattaagg ttcttaaagc
     5701 tatttctgat gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa ttggtggtgc
     5761 tgctatcgat gctacaggtg ttccacttcc agatgaggcg ctggaagcct ccaagaaggc
     5821 tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg ggaaccggta gtgttagacc
     5881 tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg tacgccaact taagaccatg
     5941 taactttgca tccgactctc ttttagactt atctccaatc aagccacaat ttgctaaagg
     6001 tactgacttc gttgttgtca gagaattagt gggaggtatt tactttggta agagaaagga
     6061 agacgatggt gatggtgtcg cttgggatag tgaacaatac accgttccag aagtgcaaag
     6121 aatcacaaga atggccgctt tcatggccct acaacatgag ccaccattgc ctatttggtc
     6181 cttggataaa gctaatgttt tggcctcttc aagattatgg agaaaaactg tggaggaaac
     6241 catcaagaac gaatttccta cattgaaggt tcaacatcaa ttgattgatt ctgccgccat
     6301 gatcctagtt aagaacccaa cccacctaaa tggtattata atcaccagca acatgtttgg
     6361 tgatatcatc tccgatgaag cctccgttat cccaggttcc ttgggtttgt tgccatctgc
     6421 gtccttggcc tctttgccag acaagaacac cgcatttggt ttgtacgaac catgccacgg
     6481 ttctgctcca gatttgccaa agaataaggt caaccctatc gccactatct tgtctgctgc
     6541 aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt aaggccattg aagatgcagt
     6601 taaaaaggtt ttggatgcag gtatcagaac tggtgattta ggtggttcca acagtaccac
     6661 cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa aagattctct
     6721 ttttttatga tatttgtaca taaactttat aaatgaaatt cataatagaa acgacacgaa
     6781 attacaaaat ggaatatgtt catagggtag acgaaactat atacgcaatc tacatacatt
     6841 tatcaagaag gagaaaaagg aggatgtaaa ggaatacagg taagcaaatt gatactaatg
     6901 gctcaacgtg ataaggaaaa agaattgcac tttaacatta atattgacaa ggaggagggc
     6961 accacacaaa aagttaggtg taacagaaaa tcatgaaact atgattccta atttatatat
     7021 tggaggattt tctctaaaaa aaaaaaatac aacaaataaa aaacactcaa tgacctgacc
     7081 atttgatgga gtttaagtca ataccttctt gaaccatttc ccataatggt gaaagttccc
     7141 tcaagaattt tactctgtca gaaacggcct taacgacgta gtcgagagta ctcgtatcgc
     7201 atgtcggtgc gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat
     7261 acgcaatcta catacattta tcaagaagga gaaaaaggag gatgtaaagg aatacaggta
     7321 agcaaattga tactaatggc tcaacgtgat aaggaaaaag aattgcactt taacattaat
     7381 attgacaagg aggagggcac cacacaaaaa gttaggtgta acagaaaatc atgaaactat
     7441 gattcctaat ttatatattg gaggattttc tctaaaaaaa aaaaaataca acaaataaaa
     7501 aacactcaat gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc
     7561 cataatggtg aaagttccct caagaatttt actctgtcag aaacggcctt aacgacgtag
     7621 tcgacctcct cttcagtact aaatctacca ataccaaatc tgatggaaga atgggctaat
     7681 gcatcatcct tacccagcgc cgagcggccg cgtgttacaa ccaattaacc aattctgatt
     7741 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     7801 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     7861 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     7921 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     7981 aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc
     8041 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     8101 cctgagcgag gcgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     8161 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     8221 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
     8281 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     8341 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     8401 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
     8461 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     8521 tggagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     8581 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     8641 gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc
     8701 agtcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8761 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8821 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8881 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8941 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9001 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9061 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9121 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9181 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9241 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9301 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9361 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9421 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtgcgg ccgccaatac
     9481 ccagaagaac agtaaaataa agcaaggtac gtgaaattaa tatttttaaa tggttctaac
     9541 cgatgccgaa gaactgcgca gtccggttat aacgtctgac atgtcctttt ttgatttgga
     9601 atccaaccac tcaagtgact ctgttcattt actttgcgaa aaatataccc acaaattgcc
     9661 catcgaaagt gaatcgcaaa ccaccttcag actggcaccg acaaagcaaa gattatacag
     9721 acagagtact ttatacgtac cgttaagtct caagcaaagg gttttcttat ttactgaacg
     9781 ggtaaagagt atctgggccg gcttgccaag atgcaaaccg aataagtatt tcaaagttgc
     9841 atttgcctta gccgtcctga caccattggc tatttggata ttttatattg actttcgtgt
     9901 acattgatca catcgactgt tctattggca aatgaaccac gggcattgac tatttttcag
     9961 gttactacta tatattatca tcacgggcaa ggattgtacc ctgaattcgc atctagactg
    10021 a
//
