LOCUS       sequence-250033-        3257 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  filamin fusion with mRFP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3257)
  TITLE     Knecht unpublished plasmids
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FLN-mRFP1
FEATURES             Location/Qualifiers
     source          1..3257
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2580..3257
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVGQYERAEGRHS
                     TGA"
     primer_bind     complement(2710..2728)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2978..2997
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3172..3195
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gaattcctcg agtaatttga agaacatgga ttgatgtcca aaaaaagact tttacaggtt
       61 gggcaaataa ttatttaaaa gaaagaattt taaagattga agatcttgca acaagtttag
      121 aagatggtgt acttttaatt aatcttttag aaattatttc aagtaaaaag attttgaaat
      181 ataacaaagc tccaaagatt agaatgcaaa agattgaaaa caacaatatg gccgttaact
      241 ttatcaagag tgaaggtttg aaattggttg gtattggtgc tgaagatatc gttgatagtc
      301 aattgaaatt aattcttggt ttaatttgga ccttgatttt acgttatcaa attcaaatgt
      361 ctgaaagtga taactcacca aaagcagctc tcttggaatg ggttagaaag caagttgccc
      421 catacaaggt tgttgtaaac aatttcacag actcatggtg tgatggtcgt gttctctctg
      481 ctctcactga ttccctcaaa ccaggtgtta gagagatgtc aaccctcacc ggtgatgccg
      541 ttcaagatat cgacagatcc atggatatcg ctcttgaaga atatgaaatc ccaaagatta
      601 tggatgccaa tgatatgaac tctcttccag atgaattatc agtcatcacc tacgtctctt
      661 atttccgtga ttatgcactc aacaaagaaa agagagatgc tgatgcttta gctgccctcg
      721 aaaagaaacg tcgtgaaact tcagatgcca gcaaggttga agtttatggt ccaggtgttg
      781 aaggtggttt cgttaataaa tccgccgatt tccacattaa agccgtcaac tactatggtg
      841 aaccattagc caacggtggt gaaggtttca ctgttagcgt cgttggtgcc gatggtgttg
      901 aagtcccatg caaattggtt gacaacaaaa atggtatcta tgatgcttca tacactgcta
      961 ccgttccaca agactacact gtcgtcgttc aattagatga tgtccactgc aaagactcac
     1021 catacaacgt taagattgac ggttcagatg ctcaacactc aaatgcctac ggtccaggtt
     1081 tagaaggtgg taaagttggt gttccagctg ctttcaaaat ccaaggtcgt aacaaggatg
     1141 gcgagaccgt cactcaaggt ggtgatgatt tcaccgtcaa agttcaatca ccagaaggtc
     1201 cagtcgatgc tcaaatcaaa gacaatggtg atggttcata cgatgttgaa tacaaaccaa
     1261 ccaaaggtgg tgatcacact gtcgaagttt tcctccgtgg tgaaccatta gcccaaggtc
     1321 caaccgaagt taagatcctc aactctgact ctcaaaacag ttattgtgat ggtccaggtt
     1381 tcgaaaaagc ccaagctaaa cgtccaactg aattcactat tcactctgtt ggtgctgata
     1441 ataaaccatg tgctgctggt ggtgatccat tccaagtatc catcagtggt ccacacccag
     1501 tcaacgttgg tatcaccgat aatgacgatg gtacttacac tgttgcctac actccagaac
     1561 aaccaggtga ctacgaaatt caagtcaccc tcaatgatga agccatcaag gatattccaa
     1621 aatcaattca tattaaacca gctgctgatc cagagaaatc atacgctgaa ggtccaggtt
     1681 tagatggtgg tgaatgtttc caaccaagca aattcaagat tcatgcagtt gatccagatg
     1741 gtgtacaccg tactgacggt ggtgacggtt ttgtcgttac cattgaaggt ccagctccag
     1801 tcgatccagt tatggtcgat aatggtgatg gtacatatga tgttgaattt gaaccaaaag
     1861 aagccggtga ctatgttatc aatctcactt tagatggtga caacgtcaat ggtttcccaa
     1921 agactgttac cgttaaacca gccccatccg ctgaacactc ttatgctgaa ggtgaaggtt
     1981 tagtcaaagt atttgataat gccccagctg aattcactat tttcgccgtt gacactaaag
     2041 gtgttgctcg taccgatggt ggtgatccat ttgaagtcgc tatcaatggt ccagatggtt
     2101 tagtcgttga tgccaaagtt accgataaca atgacggtac ttatggtgtt gtctatgatg
     2161 ccccagttga aggtaactac aatgttaatg tcaccctccg tggtaatcca atcaaaaata
     2221 tgccaatcga tgtcaaatgc attgaaggtg ccaatggtga agattcatca ttcggttcat
     2281 tcacttttac cgtcgctgct aaaaataaga aaggtgaagt taaaacctat ggtggtgata
     2341 aatttgaagt ctctatcact ggtccagctg aagaaatcac tctcgatgct attgataacc
     2401 aagatggtac ttatactgcc gcttactctt tagttggtaa tggtcgtttc tcaactggtg
     2461 tcaaattaaa cggtaaacac attgaaggtt ctccattcaa acaagtactt ggtaacccag
     2521 gtaaaaagaa tccagaagtt aaatctttca ctactactcg tactgccaat ctaggatcca
     2581 tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggct
     2641 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     2701 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
     2761 tgtcccctca gttccagtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     2821 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     2881 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     2941 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     3001 tgggctggga ggcctccacc gagcggatgt accccgagga cggcgccctg aagggcgaga
     3061 tcaagatgag gctgaagctg aaggacggcg gccactacga cgccgaggtc aagaccacct
     3121 acatggccaa gaagcccgtg cagctgcccg gcgcctacaa gaccgacatc aagctggaca
     3181 tcacctccca caacgaggac tacaccatcg tgggacagta cgagcgcgcc gagggccgcc
     3241 actccaccgg cgcctaa
//
