LOCUS       sequence-250045-        6259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For in vitro translation of human WT ELL2 with internal HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C
  TITLE     Transcription elongation factor ELL2 directs immunoglobulin 
            secretion in plasma cells by stimulating altered RNA processing.
  JOURNAL   Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009
            Sep 13.
  PUBMED    19749764
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1786"; 
            journalName: "Nat Immunol"; date: "2009-10"; volume: "10"; issue: 
            "10"; pages: "1102-9"
COMMENT     Sequence Label: HA tagged Sph ELL2
FEATURES             Location/Qualifiers
     source          1..6259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(590..601)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2109..2135
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3398..3416)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3437..3453)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3437..3453)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3450..3472)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3461..3477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3485..3491,3492..3509,3510..3515))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3530..3551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3668..3685)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3839..4427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3919..3938)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4598..5389,5390..5458))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5221..5240
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5459..5563)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5589..6044)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5726..5747)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5938..5957
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6171..6193
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6185..6202
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6186..6202
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6209..6228
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6209..6227
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatggatca ggccaaatcg gccgagctcg
       61 aattcgtcga gagcgggata agttggcgcg tcacctagac gtcagcctct tacctctcgg
      121 acgtcccgtc ccaggagcaa aactctagtg actttgggcc cgcccccgtg agagtggagg
      181 gcggccgatg agggcggttc tgaagggaac aagaccaatg agacgagccc ggggcggggc
      241 gcggggccgg agggtggggc gggtggcggc ggtggcagtc cgaggtctgg ctccgcgcag
      301 tatatgacag tacgtcagca gcgggatggc cctagcagtg gcggcggctg cagaagccca
      361 agcagccgcg gccgcagtgg aggctagagc cggagcggcg gcggcggcgg caccccgggg
      421 agtttaagat ggcggcgggg gggacagggg gcctgcggga ggagcagcgc tatgggctgt
      481 cgtgcggacg gctggggcag gacaacatca ccgtactgca tgtgaagctc accgagacgg
      541 cgatccgggc gctcgagact taccagagcc acaagaattt aattcctttt cgaccttcaa
      601 tccagttcca aggactccac gggcttgtca aaattcccaa aaatgatccc ctcaatgaag
      661 ttcataactt taacttttat ttgtcaaatg tgggcaaaga caaccctcag ggcagctttg
      721 actgcatcca gcaaacattc tccagctctg gagcctccca gctcaattgc ctgggattta
      781 tacaagataa aattacagtg tgtgcaacaa acgactcgta tcagatgaca cgagaaagaa
      841 tgacccaggc agaggaggaa tcccgcaacc gaagcacaaa agttatcaaa cccggtggac
      901 catatgtagg gaaaagagtg caaattcgga aagcacctca agctgtttca gatacagttc
      961 ctgagaggaa aaggtcaacc cccatgaacc ctgcaaatac aattcgaaag acacatagca
     1021 gcagcaccat ctctcagagg ccatacaggg acagggtgat tcacttactg gccctgaagg
     1081 cctacaagaa accggagcta cttgctagac tccagaaaga tggtgtcaat caaaaagaca
     1141 agaactccct gggagcaatt ctgcaacagg tagccaatct gaattctaag gacctctcat
     1201 ataccttaaa ggattatgtt tttaaagagc ttcaaagaga ctggcctgga tacagtgaaa
     1261 tagacagacg gtcattggag tcagtgctct ctagaaaact aaatccgtct cagaatgcta
     1321 caggcaccag ccgttcagaa tctcctgtat gttctagtag agatgctgta tcttctcctc
     1381 agaaacggct tttggattca gagtttattg atcctttaat gaataaaaaa gcccgaatat
     1441 ctcacctgac gaacagagta ccaccaacac taaatggtca tttgaatccc accagtgaaa
     1501 aatcggctgc aggcctccca ctgccccctg cggctgctgc catccccacc cctccaccgc
     1561 tgccttcaac ctatctgccc atctcacatc ctcctcagat tgtaaattct aactccaact
     1621 cccctagcac tccagaaggc cgggggactc aagacctacc tgttgacagt tttagtcaaa
     1681 acgatagtat ctatgaggac cagcaagaca aatatacctc taggacttct ctggaaacct
     1741 taccccctgg ttccgttcta ctaaagtgtc caaagcctat ggaagaaaac cattcaatgt
     1801 ctcacaaaaa gtccaaaaag aagtctaaaa aacataagga aaaggaccaa ataaaaaagc
     1861 acgacattga gactattgag gaaaaggagg aagatcttaa gagagaagag gaaattgcca
     1921 agctaaataa ctccagtcca aattccagtg gaggagttaa agaggattgc actgcctcca
     1981 tggaaccttc agcaattgaa ctcccagatt atttgataaa atatatcgct atcgtctcct
     2041 atgagcaacg ccagaattat aaggatgact tcaatgcaga gtatgatgag tacagagctt
     2101 tgcatgggta cccatacgac gtcccagact acgctgctca tgccaggatg gagactgtag
     2161 ctagaagatt tatcaaacta gatgcacaaa gaaagcgcct ttctccaggc tcaaaagagt
     2221 atcagaatgt tcatgaagaa gtcttacaag aatatcagaa gataaagcag tctagtccca
     2281 attaccatga agaaaaatac agatgtgaat atcttcataa caagctggct cacatcaaaa
     2341 ggctaatagg tgaatttgac caacagcaag cagagtcatg gtcctagaac tctgcttgga
     2401 ccagaagatg tgaataaact taagcttgga aaaaaaaaaa aaaaaaaaaa aaaaaacgcg
     2461 taacccccca ttgcaatggt tgccttaagg tgtttgctag ttgtgtacat agtgtggtta
     2521 atcattagct acactgcttc ccacttgatt agagcaatgg gaagcatact gtggcctacc
     2581 agcatctgga agtgtgtgct cgatctgtat gtgtgcagag gtggtgtgga tgtgagcgtg
     2641 catgaaggaa aaaaagctgc tactcctagt aggccaaacg ctcaggttaa acaactgacg
     2701 agtgttactg tagggtgttt ttttgttttt ttgttttttt tcttctatca aattgctact
     2761 tttgttgtgg aagacaaaag catttccatt tcaacgagtt tgtcagcttt attaatgttg
     2821 ggcaaaaatt gatatgtcat gaaaatgaaa cagatctata gttttgggac aaaattataa
     2881 aatgaaatgt gtaggtaacc tatttatata ctgctataaa gtattttttg aagagagata
     2941 tgcaaagaag ctattaccta cataagaggt atatttaaag attttttttt tttcatcctg
     3001 gtgccaggaa tataaaaaag agtggatata tttaaccata acatactgtg attcatcaaa
     3061 cagcacaaac tttcatttca tggagtttat ctgttgacat tgatttaaac tgtcacttgt
     3121 tttatcatgt gggaacataa gttatgtggt caaaaatata aggattttga attaatgttg
     3181 attcaagttg tattgtcttt tcaaagtgct gccagttgaa aagggaagca ttatgtttac
     3241 aaatctgttt tgaaatgttt gccaaaattt tggtagtgtc tttaataaag atgtttgtct
     3301 ccagcatcca gaaaaaaaaa aaaaaaanct ctccagcgct ggatccggcc ataagggcct
     3361 gatccttcga gggggggccc ggtaccagct tttgttccct ttagtgaggg ttaatttcga
     3421 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     3481 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     3541 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     3601 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     3661 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3721 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3781 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3841 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3901 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3961 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4021 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4081 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4141 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4201 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4261 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4321 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4381 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4441 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4501 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4561 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     4621 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     4681 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     4741 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4801 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4861 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4921 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4981 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5041 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5101 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5161 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5221 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5281 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5341 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5401 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5461 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5521 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     5581 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     5641 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     5701 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     5761 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     5821 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     5881 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     5941 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     6001 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     6061 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     6121 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     6181 acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg aattggagct
     6241 ccaccgcggt ggcggccgc
//
