LOCUS       sequence-250057-        8871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a human/mouse chimera that increases ELL2 expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C
  TITLE     Transcription elongation factor ELL2 directs immunoglobulin 
            secretion in plasma cells by stimulating altered RNA processing.
  JOURNAL   Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009
            Sep 13.
  PUBMED    19749764
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1786"; 
            journalName: "Nat Immunol"; date: "2009-10"; volume: "10"; issue: 
            "10"; pages: "1102-9"
COMMENT     Sequence Label: Human/mouse ELL2
FEATURES             Location/Qualifiers
     source          1..8871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2110..2127)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2116..2340
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2386..2814
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2473..2492)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2683..2704
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2823..2843)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2828..3157
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3008..3143
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3070..3089
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3205..3252
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3271..3645
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3775..3896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3812..3831)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3866..3885
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3945..3961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3945..3961)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3958..3980)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3969..3985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3993..3999,4000..4017,4018..4023))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4038..4059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4176..4193)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4347..4935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4427..4446)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5106..5897,5898..5966))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5729..5748
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5967..6071)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6146..6165)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6453..7631
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6684..7622
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7579..7599
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7648..7667
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7648..7666
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7708..7725
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7729..7761
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(7765..7773,7774..7788)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(7998..8009)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 acattaaatg gtcatttgaa tcccaccagt gaaaaatctt gtgcaggcct cctaccaccc
       61 cctgcagctg ctgccatccc caccctctcg ccgctgcctt caacccacct gcctgtctcc
      121 aatcctcctc agactgtaaa ttccaactcc aattccccta gcactccaga aggcctgggg
      181 actcaagacc tgcctgttga cagttttagt caaaatggta gcatctttga ggaccagcaa
      241 gaaaaatata cctcaaggac ttgtctggaa acattacccc ccagctcagc tctgctaaag
      301 tgtccaaagc ccatggaaga agagcatcca gtgtctcaca aaaagtccaa aaagaagtct
      361 aaaaaacaca aggaaaagga ccaaataaag aaacttgaca ttgagaccat ggaggagaag
      421 gaggaagacc ttcagagaga agaaactgcc aagctgagta atgccagtcc aaatcccaat
      481 gaaggagtta aagaagggtg cacagcctcc atggagcctt cttcagcact tgaactccca
      541 gattatttga taaaatatat tgctattgtc tcttatgagc aacgccagaa ttacaaggat
      601 gacttcaatg ctgagtatga tgaatacaga gctttgcatg caaggatgga gactgtagcc
      661 aggagattta ttaaactgga tgcacaacga aaacgccttt ctccaggttc aaaagagtac
      721 cagaatgttc atgaagaagt cttacaggaa tatcagaaga tcaagcagtc cagtcccaat
      781 taccatgaag aaaaatacag atgtgaatat cttcataaca agctggctca catcaaaaga
      841 ctaataggtg aatttgacca acagcaagca gagtcatggc actaaagctc tgcttggacc
      901 agaagatgtg aataaactaa agcttattta tttaaaattc cagatgagtt gctctagaaa
      961 actaaatcca tctcagaatg ctagcaccag ccggtcagag tctcccctgt gttccagcaa
     1021 agacgctgcc tcatctcctc agaaacgacc tttggattca gatttcattg atcctttaat
     1081 gaacaaaaaa gctcgaatat ctcacctaac aaacagagtc ccaccgacat taaatggtca
     1141 tttgaatccc accagtgaaa aatcttgtgc aggcctccta ccaccccctg cagctgctgc
     1201 catccccacc ctctcgccgc tgccttcaac ccacctgcct gtctccaatc ctcctcagac
     1261 tgtaaattcc aactccaatt cccctagcac tccagaaggc ctggggactc aagacctgcc
     1321 tgttgacagt tttagtcaaa atggtagcat ctttgaggac cagcaagaaa aatatacctc
     1381 aaggacttgt ctggaaacat taccccccag ctcagctctg ctaaagtgtc caaagcccat
     1441 ggaagaagag catccagtgt ctcacaaaaa gtccaaaaag aagtctaaaa aacacaagga
     1501 aaaggaccaa ataaagaaac ttgacattga gaccatggag gagaaggagg aagaccttca
     1561 gagagaagaa actgccaagc tgagtaatgc cagtccaaat cccaatgaag gagttaaaga
     1621 agggtgcaca gcctccatgg agccttcttc agcacttgaa ctcccagatt atttgataaa
     1681 atatattgct attgtctctt atgagcaacg ccagaattac aaggatgact tcaatgctga
     1741 gtatgatgaa tacagagctt tgcatgcaag gatggagact gtagccagga gatttattaa
     1801 actggatgca caacgaaaac gcctttctcc aggttcaaaa gagtaccaga atgttcatga
     1861 agaagtctta caggaatatc agaagatcaa gcagtccagt cccaattacc atgaagaaaa
     1921 atacagatgt gaatatcttc ataacaagct ggctcacatc aaaagactaa taggtgaatt
     1981 tgaccaacag caagcagagt catggcacta aagctctgct tggaccagaa gatgtgaata
     2041 aactaaagct tatttattta aaattccaga tgagttgctc tagagggccc gtttaaaccc
     2101 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     2161 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     2221 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     2281 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     2341 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     2401 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2461 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2521 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2581 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     2641 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2701 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     2761 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     2821 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     2881 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     2941 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3001 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3061 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     3121 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     3181 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     3241 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     3301 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     3361 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     3421 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     3481 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     3541 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     3601 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     3661 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     3721 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     3781 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3841 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3901 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     3961 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4021 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4081 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4141 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4201 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4261 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4321 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4381 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4441 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4501 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4561 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4621 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4681 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4741 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4801 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4861 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4921 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4981 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5041 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5101 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5161 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5221 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5281 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5341 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5401 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5461 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5521 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5581 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5641 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5701 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5761 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5821 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5881 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5941 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6001 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6061 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     6121 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     6181 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     6241 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     6301 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actaggcttt
     6361 tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat
     6421 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc
     6481 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     6541 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     6601 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     6661 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
     6721 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
     6781 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     6841 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     6901 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     6961 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     7021 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     7081 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     7141 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     7201 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     7261 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     7321 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     7381 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     7441 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     7501 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     7561 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     7621 aggtgtcgtg aggaattagc ttggtactaa tacgactcac tatagggaga cccaagctgg
     7681 ctaggtaagc ttaccatggg gggttctcat catcatcatc atcatggtat ggctagcatg
     7741 actggtggac agcaaatggg tcgggatctg tacgacgatg acgataaggt acctaaggat
     7801 ccagtgtggt ggaattctgc agatggggag tttaagatgg cggcgggggg gacagggggc
     7861 ctgcgggagg agcagcgcta tgggctgtcg tgcggacggc tggggcagga caacatcacc
     7921 gtactgcatg tgaagctcac cgagacggcg atccgggcgc tcgagactta ccagagccac
     7981 aagaatttaa ttccttttcg accttcaatc cagttccaag gactccacgg gcttgtcaaa
     8041 attcccaaaa atgatcccct caatgaagtt cataacttta acttttattt gtcaaatgtg
     8101 ggcaaagaca accctcaggg cagctttgac tgcatccagc aaacattctc cagctctgga
     8161 gcctcccagc tcaattgcct gggatttata caagataaaa ttacagtgtg tgcaacaaac
     8221 gactcgtatc agatgacacg agaaagaatg acccaggcag aggaggaatc ccgcaaccga
     8281 agcacaaaag ttatcaaacc cggtggacca tatgtaggga aaagagtgca aattcggaaa
     8341 gcacctcaag ctgtttcaga tacagttcct gagaggaaaa ggtcaacccc catgaaccct
     8401 gcaaatacaa ttcgaaagac acatagcagc agcaccatct ctcagaggcc atacagggac
     8461 agggtgattc acttactggc cctgaaggcc tacaagaaac cggagctact tgctagactc
     8521 cagaaagatg gtgtcaatca aaaagacaag aactccctgg gagcaattct gcaacaggta
     8581 gccaatctga attctaagga cctctcatat accttaaagg attatgtttt taaagagctt
     8641 caaagagact ggcctggata cagtgaaata gacagacggt cattggagtc agtgctctct
     8701 agaaaactaa atccatctca gaatgctagc accagccggt cagagtctcc cctgtgttcc
     8761 agcaaagacg ctgcctcatc tcctcagaaa cgacctttgg attcagattt cattgatcct
     8821 ttaatgaaca aaaaagctcg aatatctcac ctaacaaaca gagtcccacc g
//
