LOCUS       sequence-250064-        8662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual expression vector for mitochondrial matrix and outer membrane 
            Ca2+ sensors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Ashrafi G, de Juan-Sanz J, Farrell RJ, Ryan TA
  TITLE     Molecular Tuning of the Axonal Mitochondrial Ca(2+) Uniporter 
            Ensures Metabolic Flexibility of Neurotransmission.
  JOURNAL   Neuron. 2019 Dec 10. pii: S0896-6273(19)30984-5. doi: 
            10.1016/j.neuron.2019.11.020.
  PUBMED    31862210
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2019 Dec 10. pii: S0896-6273(19)30984-5. doi: 
            10.1016/j.neuron.2019.11.020."
COMMENT     Sequence Label: CMV-OMMjRCaMP-P2A-mito4xGCaMP6f
FEATURES             Location/Qualifiers
     source          1..8662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      135..270
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     197..216
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             351..1145
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(405..424)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1015..1034
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1319..1440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1356..1375)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1410..1429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1489..1505)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1489..1505)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1502..1524)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1513..1529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1537..1543,1544..1561,1562..1567))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1582..1603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1720..1737)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1891..2479)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1971..1990)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2650..3441,3442..3510))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3273..3292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3511..3615)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3690..3709)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3881..4260
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4261..4464
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4415..4435
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4509..4528
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4509..4527
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4661..4678
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(4718..4726,4727..4741)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             5984..6040
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6053..6127
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             6149..6223
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             6245..6319
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             6341..6415
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             6473..6490
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(6530..6538,6539..6553)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             6581..6637
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     complement(7238..7257)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(7899..7916)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7905..8129
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8175..8603
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8262..8281)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8472..8493
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8612..8632)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(8617..8662,1..284)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
       61 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
      121 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
      181 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
      241 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
      301 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
      361 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
      421 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
      481 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
      541 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
      601 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
      661 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
      721 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
      781 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
      841 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
      901 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
      961 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     1021 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     1081 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     1141 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     1201 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     1261 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     1321 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1381 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     1441 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     1501 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     1561 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     1621 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     1681 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1741 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1801 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1861 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1921 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1981 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2041 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2101 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2161 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2221 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2281 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2341 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2401 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2461 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2521 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2581 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2641 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2701 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2761 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2821 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2881 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2941 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3001 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3061 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3241 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3361 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     3661 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     3721 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     3781 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     3841 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     3901 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     3961 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4021 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4081 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     4141 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4201 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4261 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     4321 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     4381 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     4441 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
     4501 atcgaaatta atacgactca ctatagggag acccaagctg gctagcaccg ccatggtggg
     4561 aagaaactct gctatcgccg ctggcgtttg tggcgccctg ttcatcggct actgcatcta
     4621 cttcgacaga aagaggcgga gcgaccccaa caccggttct caccaccatc accatcacgg
     4681 catggcctct atgacaggcg gacagcagat gggcagagat ctgtacgacg acgatgacaa
     4741 ggacctggcc acaatggtgg acagcagcag gcggaagtgg aacaaatggg gacacgccgt
     4801 cagagccatc ggcagattgt ctagcgccaa caacaccgag atgatgtacc ctgctgacgg
     4861 cggcctgaga ggctacacac atatggccct gaaagtggac ggcggaggcc acctgtcttg
     4921 tagcttcgtg accacctaca ggtccaagaa aaccgtgggc aacatcaaga tgcccgccat
     4981 ccactacgtg tcccacaggc tggaaagact ggaagagtcc gacaacgaga tgttcgtggt
     5041 gcagagggaa cacgccgtgg ccaagtttgt tggactcgga ggtggcggag gaaccggcgg
     5101 atctatgaac agcctgatca aagaaaacat gcggatgaag gtggtgctgg aaggcagcgt
     5161 gaacggccac cagttcaagt gtacaggcga aggcgagggc aacccctaca tgggcacaca
     5221 gaccatgagg atcaaagtga tcgaaggcgg ccctctgcct ttcgctttcg acatcctggc
     5281 caccagcttt atgtacggca gcaggacctt catcaagtac cccaagggaa tccccgactt
     5341 cttcaagcag agcttccccg agggcttcac ctgggagaga gtgaccagat acgaggacgg
     5401 cggcgtgatc acagtgatgc aggacacctc tctggaagat ggctgcctgg tgtaccacgt
     5461 gcaagtgcgg ggagtgaact tcccttctaa cggggccgtg atgcagaaaa agaccaaagg
     5521 ctgggagccc accgacagcc agctgacaga agaacagatc gccgagttca aagaggcctt
     5581 cagcctgttc gacaaggacg gcgacggcac aatcaccacc aaagaaatgg gcaccgtgat
     5641 gagaagcctg ggacagaacc ctacagaggc cgagctgcag gacatgatca acgaggttga
     5701 cgccgatggc gacggaacta tcgacttccc tgagttcctg atcatgatgg ccggcaagat
     5761 gaagtacacc gactccgagg aagagatccg cgaggccttc ggagtgtttg ataaggacgg
     5821 aaacggctac atctctgccg ccgagctgag acacgtgatg accaatctgg gcgagaagct
     5881 gaccgacgaa gaggtggacg agatgatcag agaggccgat tcagacggcg acgggcaagt
     5941 gaactacgaa gagttcgtgc agatgatgac cgccaagacc ggtgccacca actttagcct
     6001 gctgaaacag gccggcgacg tggaagagaa tcctggacct ggatccaccg ccatgagcgt
     6061 gctgacacct ctgctgctga gaggcctgac aggctctgct agaaggctgc ctgtgcctag
     6121 agccaagatc cactctctgg gcgaccctat gtctgtgctg actccactgc tcctgcgggg
     6181 actgactgga tctgctcgta gactgccagt gccacgggct aagattcaca gcctgggcga
     6241 tccaatgagt gtgcttaccc cactcctgct cagaggactg accggaagtg ctagacgcct
     6301 gccagttcca agggccaaaa tccatagtct gggagatccc atgtccgtgt tgacacccct
     6361 cttgctgagg ggacttaccg gctcagctcg gagactccct gttccaagag caaagattca
     6421 ttccctcggc gacccaagat ctggcagcgc taaagaccct accatgggta gccaccacca
     6481 ccatcaccac ggcatggctt ctatgacagg cggccagcag atgggccgcg acctgtacga
     6541 tgacgacgat aaggacctgg ccacaatggt ggacagcagc aggcggaagt ggaacaagac
     6601 aggccacgcc gttagagcca tcggcagact gtctagcctg gaaaacgtgt acatcaaggc
     6661 cgacaagcag aagaacggca tcaaggctaa cttcaagatc cggcacaaca tcgaggacgg
     6721 cggagtgcag ctggcttacc actaccagca gaacacccct atcggcgacg gaccagttct
     6781 gctgcctgac aaccactacc tgagcgtgca gagcaagctg agcaaggacc ccaacgagaa
     6841 gagggaccac atggtgctgc tggaatttgt gaccgccgct ggcatcacac tcggcatgga
     6901 cgaactgtac aaaggcggca caggcggctc tatggtgtcc aagggcgaag aactgttcac
     6961 cggcgtggtg cccattctgg tggaactgga tggggatgtg aacggccaca agttcagcgt
     7021 cagcggagaa ggcgaaggcg acgccacata cggaaagctg accctgaagt tcatctgcac
     7081 caccggcaaa ctgcccgtgc cttggcctac actggtcacc acactgacat acggcgtgca
     7141 gtgcttcagc agataccccg accatatgaa gcagcacgac ttcttcaaga gcgccatgcc
     7201 tgagggctac atccaagaga gaaccatctt ctttaaggac gacggcaact acaagaccag
     7261 ggccgaagtg aagttcgagg gcgacaccct ggtcaacaga atcgagctga agggcatcga
     7321 cttcaaagag gacggcaaca tcctgggcca caagctcgag tacaacctgc ctgaccagct
     7381 gaccgaggaa cagatcgccg agttcaaaga agagttcagc ctgttcgaca aggacggcga
     7441 cggcacaatc accaccaaag aactgggcac cgtgatgaga agcctgggac agaaccctac
     7501 agaggccgag ctgcaggaca tgatcaacga ggttgacgct gatggcgacg gaactatcga
     7561 cttccccgag ttcctgacca tgatggccag aaagatgaag tacagggaca cagaggaaga
     7621 gatccgcgag gccttcggcg tgttcgataa ggatggaaac ggctacatct ctgccgctga
     7681 gctgagacac gtgatgacca acctgggcga gaagctgaca gacgaagagg tggacgagat
     7741 gatcagagag gccgacattg acggcgacgg gcaagtgaac tacgaagagt ttgtccagat
     7801 gatgaccgcc aagtgaaccg gtgtcgacga attctgcaga tatccagcac agtggcggcc
     7861 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     7921 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     7981 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8041 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8101 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     8161 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     8221 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     8281 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     8341 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     8401 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     8461 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     8521 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     8581 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     8641 tccccaggct ccccagcagg ca
//
