LOCUS       sequence-250093-        1185 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial 
            membrane of planta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Kuhnert F, Stefanski A, Overbeck N, Drews L, Reichert AS, Stuhler K,
            Weber APM
  TITLE     Rapid Single-Step Affinity Purification of HA-Tagged Plant 
            Mitochondria.
  JOURNAL   Plant Physiol. 2020 Feb;182(2):692-706. doi: 10.1104/pp.19.00732. 
            Epub 2019 Dec 9.
  PUBMED    31818904
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.19.00732"; journalName: "Plant Physiol"; date: 
            "2020-02"; volume: "182"; issue: "2"; pages: "692-706"
COMMENT     Sequence Label: pUTKan-3xHA-GFP-TOM5
FEATURES             Location/Qualifiers
     source          1..1185
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             190..279
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             join(298..300,301..1011)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(340..361)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(601..620)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     948..969
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tctcgattag ggtttcatag atatcatccg atttgttcaa ataatttgag ttttgtcgaa
       61 taattactct tcgatttgtg atttctatct agatctggtg ttagtttcta gtttgtgcga
      121 tcgaatttgt agattaatct gagtttttct gattaacact cgagtgcggg atcctctaga
      181 gggcccatgt acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac
      241 tatgcaggat cctatccata tgacgttcca gattacgctc cgcggggagg gagcggcgtg
      301 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      361 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      421 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      481 accaccttca cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      541 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      601 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      661 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      721 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
      781 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      841 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      901 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      961 gagttcgtga ccgccgccgg gatcactcac ggcatggacg agctgtacaa gggagggagc
     1021 ggcgtcgacg tgaacaacgt tgtctctatc gaaaagatga aagcactctg gcactccgag
     1081 gttcatgatg aacaaaaatg ggcggtgaac atgaaacttc tgcgagcact tggtatgttt
     1141 gcaggaggag tcgtcctcat gcgtagctat ggggatctca tggga
//
