LOCUS       sequence-250094-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial 
            membrane of planta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Kuhnert F, Stefanski A, Overbeck N, Drews L, Reichert AS, Stuhler K,
            Weber APM
  TITLE     Rapid Single-Step Affinity Purification of HA-Tagged Plant 
            Mitochondria.
  JOURNAL   Plant Physiol. 2020 Feb;182(2):692-706. doi: 10.1104/pp.19.00732. 
            Epub 2019 Dec 9.
  PUBMED    31818904
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.19.00732"; journalName: "Plant Physiol"; date: 
            "2020-02"; volume: "182"; issue: "2"; pages: "692-706"
COMMENT     Sequence Label: pUTKan-3xHA-GFP-TOM5
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..56
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(75..77,78..788)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(117..138)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(378..397)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     725..746
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cctatgacgt cccggactat gcaggatcct atccatatga cgttccagat tacgctccgc
       61 ggggagggag cggcgtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      121 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      181 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      241 cctggcccac cctcgtgacc accttcacct acggcgtgca gtgcttcagc cgctaccccg
      301 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      361 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      421 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
      481 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
      541 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
      601 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      661 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
      721 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactcacggc atggacgagc
      781 tgtacaaggg agggagcggc gtcgacgtga acaacgttgt ctctatcgaa aagatgaaag
      841 cactctggca ctccgaggtt catgatgaac aaaaatgggc ggtgaacatg aaacttctgc
      901 gagcacttgg tatgtttgca ggaggagtcg tcctcatgcg tagctatggg gatctcatgg
      961 gagtttgatg aactagttgg ctgcagagct ttcgttcg
//
