LOCUS       sequence-250149-        8798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  4x 1545 b long scaffolds divided by Zn-dependent self-cleaving 
            DNAzyme cassettes, from compatibility group 10, made by method IV, 
            yields linear 1033 b long scaffolds after Zn incubation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Engelhardt FAS, Praetorius F, Wachauf CH, Bruggenthies G, Kohler F, 
            Kick B, Kadletz KL, Pham PN, Behler KL, Gerling T, Dietz H
  TITLE     Custom-Size, Functional, and Durable DNA Origami with 
            Design-Specific Scaffolds.
  JOURNAL   ACS Nano. 2019 Apr 22. doi: 10.1021/acsnano.9b01025.
  PUBMED    30990672
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Nano. 
            2019 Apr 22. doi: 10.1021/acsnano.9b01025."
COMMENT     Sequence Label: pre_IV_CS10_1545_v1
FEATURES             Location/Qualifiers
     source          1..8798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6978..7566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7058..7077)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7737..8528,8529..8597))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8360..8379
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8598..8702)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8770..8788
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cctccccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
       61 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc aagcttgtca
      121 ctccggaagt tagttgagct gtcacagaat gtgacgttga agacttccgg cttaagctct
      181 gaaagggttc tatatctcca ggtagatctg ctcccaatgt aacatgctcg ggacctacaa
      241 ggtgtcagga tcgaagattg cacgacgatg acttacggac agccggacgt actccctgaa
      301 caatgcgatt cgatatacac ggtggtgttc tgtttgggcc ttctgactca aagccaagcc
      361 tggataacag tgtccttcgg gtcctccgtg ttatggggca cggctgctta gagcattgcc
      421 acggagcctt tgaaggcagc gagggcgtgg cctcccgacc gcactagcac aacacaggag
      481 aggaccggcg acatacctgg gtggaagttt catcggaata ctcgtcaaac gactaaccca
      541 tcagccatcg atcgtatgaa tatgtataat ccatccgtac ctagagtcgc gggccagcat
      601 caacgcatgt ggtaaattgg ttggatccgc gagcagtaag aacccttaaa tcaagcttcc
      661 cggcacagcg tcagtggcgg atacgcgcga tgtctgccgt gctgaagtag ggtaagggcc
      721 ctccatgcgg acttcttggt agctccaaga gtggattccc tcgtaagttc ggctacgtca
      781 tttactgcag cctctgcaag cggaggaact ccactatgca cttagggact actgatatca
      841 aattcgccgc cgaaacccgc aagatggtgc tctttaactg gccaatacat agcccagtga
      901 aactgttcgc atatggcgaa cctttctgcg ccacttgttt cgcgcacgtt taggagctta
      961 ttagctaaat gaaccagttc tctggagtga tacagacttg cggcgtcccg taaaccgaga
     1021 gtaacttaac cataaagctg gtgggtatag aacatatagc gatcagtacg gccatttgac
     1081 ggcaaatacc gtctggctgg caagtcgggc tgatgaaatg cctgacagaa cttgaactga
     1141 ccagaagtga gcaaaggcgg tctcaattaa cactttccta tacgacctcc tcttcagagg
     1201 gtcatgagga gtctctacct cgcgaaggga gggaagccgc agcagaccct atcagggatc
     1261 ataagcgcgc tattgcgaat ttctccgaca gttgtagagg ttgctcaact agacattagt
     1321 agcaggcatg cagggtgatt aaacgggcat cgcgttaggc tttagcatgg cccatgaagc
     1381 gtacgtggtc agaataaata acctatgggc gccttatgcg cgtgtgatgt tagatatgat
     1441 tcacctgtcg gtgcaacgta atataactct cgagacacac ataacgtcgc ccgcgtctag
     1501 ctgcacattt caagacgctc atgccagggc aggacgcgac tgcgttgagg ctgtatgttg
     1561 gtgaacgcgt atctgtaaag gtacgagtca taggacacta attcatacaa cggcgcttgc
     1621 atactggtct aagaccgaat ggaaacgtta ctaaggaggc accctcagac aggttcgtcc
     1681 tgatctcgcc tcggatgaac gttgaagcgt tacctgttag gtaacgtagt tgagctgttc
     1741 atccgatttg gttccggaag ttagttgagc tgtcacagaa tgtgacgttg aagacttccg
     1801 gcttaagctc tgaaagggtt ctatatctcc aggtagatct gctcccaatg taacatgctc
     1861 gggacctaca aggtgtcagg atcgaagatt gcacgacgat gacttacgga cagccggacg
     1921 tactccctga acaatgcgat tcgatataca cggtggtgtt ctgtttgggc cttctgactc
     1981 aaagccaagc ctggataaca gtgtccttcg ggtcctccgt gttatggagc acggctgctt
     2041 agagcattgc cacggagcct ttgaaggcag cgagggcgtg gcctcccgac cgcactagca
     2101 caacacagga gaggaccggc gacatacctg ggtggaagtt tcatcggaat actcgtcaaa
     2161 cgactaaccc atcagccatc gatcgtatga atatgtataa tccatccgta cctagagtcg
     2221 cgggccagca tcaacgcatg tggtaaattg gttggatccg cgagcagtaa gaacccttaa
     2281 atcaagcttc ccggcacagc gtcagtggcg gatacgcgcg atgtctgccg tgctgaagta
     2341 gggtaagggc cctccatgcg gacttcttgg tagctccaag agtggattcc ctcgtaagtt
     2401 cggctacgtc atttactgca gcctctgcaa gcggaggaac tccactatgc acttagggac
     2461 tactgatatc aaattcgccg ccgaaacccg caagatggtg ctctttaact ggccaataca
     2521 tagcccagtg aaactgttcg catatggcga acctttctgc gccacttgtt tcgcgcacgt
     2581 ttaggagctt attagctaaa tgaaccagtt ctctggagtg atacagactt gcggcgtccc
     2641 gtaaaccgag agtaacttaa ccataaagct ggtgggtata gaacatatag cgatcagtac
     2701 ggccatttga cggcaaatac cgtctggctg gcaagtcggg ctgatgaaat gcctgacaga
     2761 acttgaactg accagaagtg agcaaaggcg gtctcaatta acactttcct atacgacctc
     2821 ctcttcagag ggtcatgagg agtctctacc tcgcgaaggg agggaagccg cagcagaccc
     2881 tatcagggat cataagcgcg ctattgcgaa tttctccgac agttgtagag gttgctcaac
     2941 tagacattag tagcaggcat gcagggtgat taaacgggca tcgcgttagg ctttagcatg
     3001 gcccatgaag cgtacgtggt cagaataaat aacctatggg cgccttatgc gcgtgtgatg
     3061 ttagatatga ttcacctgtc ggtgcaacgt aatataactc tcgagacaca cataacgtcg
     3121 cccgcgtcta gctgcacatt tcaagacgct catgccaggg caggacgcga ctgcgttgag
     3181 gctgtatgtt ggtgaacgcg tatctgtaaa ggtacgagtc ataggacact aattcataca
     3241 acggcgcttg catactggtc taagaccgaa tggaaacgtt actaaggagg caccctcaga
     3301 caggttcgtc ctgatctcgc ctcggatgaa cgttgaagcg ttacctgtta ggtaacgtag
     3361 ttgagctgtt catccgattt mcttccggaa gttagttgag ctgtcacaga atgtgacgtt
     3421 gaagacttcc ggcttaagct ctgaaagggt tctatatctc caggtagatc tgctcccaat
     3481 gtaacatgct cgggacctac aaggtgtcag gatcgaagat tgcacgacga tgacttacgg
     3541 acagccggac gtactccctg aacaatgcga ttcgatatac acggtggtgt tctgtttggg
     3601 ccttctgact caaagccaag cctggataac agtgtccttc gggtcctccg tgttatggag
     3661 cacggctgct tagagcattg ccacggagcc tttgaaggca gcgagggcgt ggcctcccga
     3721 ccgcactagc acaacacagg agaggaccgg cgacatacct gggtggaagt ttcatcggaa
     3781 tactcgtcaa acgactaacc catcagccat cgatcgtatg aatatgtata atccatccgt
     3841 acctagagtc gcgggccagc atcaacgcat gtggtaaatt ggttggatcc gcgagcagta
     3901 agaaccctta aatcaagctt cccggcacag cgtcagtggc ggatacgcgc gatgtctgcc
     3961 gtgctgaagt agggtaaggg ccctccatgc ggacttcttg gtagctccaa gagtggattc
     4021 cctcgtaagt tcggctacgt catttactgc agcctctgca agcggaggaa ctccactatg
     4081 cacttaggga ctactgatat caaattcgcc gccgaaaccc gcaagatggt gctctttaac
     4141 tggccaatac atagcccagt gaaactgttc gcatatggcg aacctttctg cgccacttgt
     4201 ttcgcgcacg tttaggagct tattagctaa atgaaccagt tctctggagt gatacagact
     4261 tgcggcgtcc cgtaaaccga gagtaactta accataaagc tggtgggtat agaacatata
     4321 gcgatcagta cggccatttg acggcaaata ccgtctggct ggcaagtcgg gctgatgaaa
     4381 tgcctgacag aacttgaact gaccagaagt gagcaaaggc ggtctcaatt aacactttcc
     4441 tatacgacct cctcttcaga gggtcatgag gagtctctac ctcgcgaagg gagggaagcc
     4501 gcagcagacc ctatcaggga tcataagcgc gctattgcga atttctccga cagttgtaga
     4561 ggttgctcaa ctagacatta gtagcaggca tgcagggtga ttaaacgggc atcgcgttag
     4621 gctttagcat ggcccatgaa gcgtacgtgg tcagaataaa taacctatgg gcgccttatg
     4681 cgcgtgtgat gttagatatg attcacctgt cggtgcaacg taatataact ctcgagacac
     4741 acataacgtc gcccgcgtct agctgcacat ttcaagacgc tcatgccagg gcaggacgcg
     4801 actgcgttga ggctgtatgt tggtgaacgc gtatctgtaa aggtacgagt cataggacac
     4861 taattcatac aacggcgctt gcatactggt ctaagaccga atggaaacgt tactaaggag
     4921 gcaccctcag acaggttcgt cctgatctcg cctcggatga acgttgaagc gttacctgtt
     4981 aggtaacgta gttgagctgt tcatccgatt tggttccgga agttagttga gctgtcacag
     5041 aatgtgacgt tgaagacttc cggcttaagc tctgaaaggg ttctatatct ccaggtagat
     5101 ctgctcccaa tgtaacatgc tcgggaccta caaggtgtca ggatcgaaga ttgcacgacg
     5161 atgacttacg gacagccgga cgtactccct gaacaatgcg attcgatata cacggtggtg
     5221 ttctgtttgg gccttctgac tcaaagccaa gcctggataa cagtgtcctt cgggtcctcc
     5281 gtgttatgga gcacggctgc ttagagcatt gccacggagc ctttgaaggc agcgagggcg
     5341 tggcctcccg accgcactag cacaacacag gagaggaccg gcgacatacc tgggtggaag
     5401 tttcatcgga atactcgtca aacgactaac ccatcagcca tcgatcgtat gaatatgtat
     5461 aatccatccg tacctagagt cgcgggccag catcaacgca tgtggtaaat tggttggatc
     5521 cgcgagcagt aagaaccctt aaatcaagct tcccggcaca gcgtcagtgg cggatacgcg
     5581 cgatgtctgc cgtgctgaag tagggtaagg gccctccatg cggacttctt ggtagctcca
     5641 agagtggatt ccctcgtaag ttcggctacg tcatttactg cagcctctgc aagcggagga
     5701 actccactat gcacttaggg actactgata tcaaattcgc cgccgaaacc cgcaagatgg
     5761 tgctctttaa ctggccaata catagcccag tgaaactgtt cgcatatggc gaacctttct
     5821 gcgccacttg tttcgcgcac gtttaggagc ttattagcta aatgaaccag ttctctggag
     5881 tgatacagac ttgcggcgtc ccgtaaaccg agagtaactt aaccataaag ctggtgggta
     5941 tagaacatat agcgatcagt acggccattt gacggcaaat accgtctggc tggcaagtcg
     6001 ggctgatgaa atgcctgaca gaacttgaac tgaccagaag tgagcaaagg cggtctcaat
     6061 taacactttc ctatacgacc tcctcttcag agggtcatga ggagtctcta cctcgcgaag
     6121 ggagggaagc cgcagcagac cctatcaggg atcataagcg cgctattgcg aatttctccg
     6181 acagttgtag aggttgctca actagacatt agtagcaggc atgcagggtg attaaacggg
     6241 catcgcgtta ggctttagca tggcccatga agcgtacgtg gtcagaataa ataacctatg
     6301 ggcgccttat gcgcgtgtga tgttagatat gattcacctg tcggtgcaac gtaatataac
     6361 tctcgagaca cacataacgt cgcccgcgtc tagctgcaca tttcaagacg ctcatgccag
     6421 ggcaggacgc gactgcgttg aggctgtatg ttggtgaacg cgtatctgta aaggtacgag
     6481 tcataggaca ctaattcata caacggcgct tgcatactgg tctaagaccg aatggaaacg
     6541 ttactaagga ggcaccctca gacaggttcg tcctgatctc gcctcggatg aacgttgaag
     6601 cgttacctgt taggtaacgt agttgagctg ttcatccgat tcattgaccg ggagcgccct
     6661 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6721 ccagcgccct agcgcccgct cccgggatcg gaattccggc catcgccctg atagacggtt
     6781 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6841 aaacgtctag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6901 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6961 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
     7021 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7081 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7141 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7201 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7261 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7321 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7381 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7441 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7501 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7561 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7621 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7681 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7741 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7801 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7861 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7921 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7981 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8041 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8101 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8161 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8221 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8281 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8341 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8401 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8461 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8521 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8581 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8641 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8701 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     8761 acctataaaa ataggcgtat cacgaggcag aatttcag
//
