LOCUS       sequence-250212-        5282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mutant of mmPylRS designed for incorporation of non-canonical amino 
            acids (acyl-lysine derivatives) in to M13 bacteriophage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Wang XS, Chen PC, Hampton JT, Tharp JM, Reed CA, Das SK, Wang DS, 
            Hayatshahi HS, Shen Y, Liu J, Liu WR
  TITLE     A Genetically Encoded, Phage-Displayed Cyclic-Peptide Library.
  JOURNAL   Angew Chem Int Ed Engl. 2019 Oct 28;58(44):15904-15909. doi: 
            10.1002/anie.201908713. Epub 2019 Sep 9.
  PUBMED    31398275
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/anie.201908713"; journalName: "Angew Chem Int Ed Engl"; 
            date: "2019-10-28- 28"; volume: "58"; issue: "44"; pages: 
            "15904-15909"
COMMENT     Sequence Label: pEVOL-AcKRS-CloDF
FEATURES             Location/Qualifiers
     source          1..5282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             complement(2339..2998)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2913..2932
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2999..3101)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      complement(3503..4241)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(4378..5256)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPSDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 ctaacaggag gaattactag tatggataaa aaaccactaa acactctgat atctgcaacc
      361 gggctctgga tgtccaggac cggaacaatt cataaaataa aacaccacga agtctctcga
      421 agcaaaatct atattgaaat ggcatgcgga gaccaccttg ttgtaaacaa ctccaggagc
      481 agcaggactg caagagcgct caggcaccac aaatacagga agacctgcaa acgctgcagg
      541 gtttcggatg aggatctcaa taagttcctc acaaaggcaa acgaagacca gacaagcgta
      601 aaagtcaagg tcgtttctgc ccctaccaga acgaaaaagg caatgccaaa atccgttgcg
      661 agagccccga aacctcttga gaatacagaa gcggcacagg ctcaaccttc tggatctaaa
      721 ttttcacctg cgataccggt ttccacccaa gagtcagttt ctgtcccggc atctgtttca
      781 acatcaatat caagcatttc tacaggagca actgcatccg cactggtaaa agggaatacg
      841 aatcccatta catccatgtc tgcccctgtt caggcaagtg cccccgcact tacgaagagc
      901 cagactgaca ggcttgaagt cctgttaaac ccaaaagatg agatttccct gaattccggc
      961 aagcctttca gggagcttga gtccgaattg ctctctcgca gaaaaaaaga cctgcagcag
     1021 atctacgcgg aagaaaggga gaattatctg gggaaactcg agcgtgaaat taccaggttc
     1081 tttgtggaca ggggttttct ggaaataaaa tccccgatcc tgatccctct tgagtatatc
     1141 gaaaggatgg gcattgataa tgataccgaa ctttcaaaac agatcttcag ggttgacaag
     1201 aacttctgcc tgagacccat gatggctcca aacctgctga actacgcccg caagcttgac
     1261 agggccctgc ctgatccaat aaaaattttt gaaataggcc catgctacag aaaagagtcc
     1321 gacggcaaag aacacctcga agagtttacc atgctgaact tcttccagat gggatcggga
     1381 tgtacacggg aaaatcttga aagcataatt acggacttcc tgaaccacct gggaattgat
     1441 ttcaagatcg taggcgattc ctgcatggtc tatggggata cccttgatgt aatgcacgga
     1501 gacctggaac tttcctctgc agtagtcgga cccataccgc ttgaccggga atggggtatt
     1561 gataaaccct ggataggggc aggtttcggg ctcgaacgcc ttctaaaggt taaacacgac
     1621 tttaaaaata tcaagagagc tgcaaggtcc gagtcttact ataacgggat ttctaccaac
     1681 ctgtgagcgg ccgctttcaa acgctaaatt gcctgatgcg ctacgcttat caggcctaca
     1741 tgatctctgc aatatattga gtttgcgtgc ttttgtaggc cggataaggc gttcacgccg
     1801 catccggcaa gaaacagcaa acaatccaaa acgccgcgtt cagcggcgtt ttttctgctt
     1861 ttcttcgcga attaattccg cttcgcaaca tgtgagcacc ggtttattga ctaccggaag
     1921 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga
     1981 ggtaataatt gacgatatga tcagtgcacg gctaactaag cggcctgctg actttctcgc
     2041 cgatcaaaag gcattttgct attaagggat tgacgagggc gtatctgcgc agtaagatgc
     2101 gccccgcatt ggaaacctga tcatgtagat cgaatggact ctaaatccgt tcagccgggt
     2161 tagattcccg gggtttccgc caaattcgaa aagcctgctc aacgagcagg cttttttgca
     2221 tgctcgagca gctcagggtc gaatttgctt tcgaatttct gccattcatc cgcttattat
     2281 cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct taaaaaaatt
     2341 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     2401 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     2461 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     2521 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     2581 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     2641 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     2701 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     2761 cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa
     2821 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     2881 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     2941 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     3001 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     3061 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa
     3121 agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt
     3181 gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct gccaacttac
     3241 tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt
     3301 ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc cggacatcag
     3361 cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc
     3421 ttcatgtggc aggagaaaaa aggggcgcgc ccaaatagct agctcactcg gtcgctacgc
     3481 tccgggcgtg agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt
     3541 ggataagcag gggactaaca tgtgaggcaa aacagcaggg ccgcgccggt ggcgtttttc
     3601 cataggctcc gccctcctgc cagagttcac ataaacagac gcttttccgg tgcatctgtg
     3661 ggagccgtga ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag
     3721 atccccaccg tttccggcgg gtcgctccct cttgcgctct cctgttccga ccctgccgtt
     3781 taccggatac ctgttccgcc tttctccctt acgggaagtg tggcgctttc tcatagctca
     3841 cacactggta tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac
     3901 tccccgttca gcccgactgc tgcgccttat ccggtaactg ttcacttgag tccaacccgg
     3961 aaaagcacgg taaaacgcca ctggcagcag ccattggtaa ctgggagttc gcagaggatt
     4021 tgtttagcta aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct acactggaag
     4081 gacagatttg gttgctgtgc tctgcgaaag ccagttacca cggttaagca gttccccaac
     4141 tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga
     4201 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact gaaccgctct
     4261 agatttcagt gcaatttatc cctaggtctt caaatgtagc acctgaagtc agccccatac
     4321 gatataagtt gtaattctca tgtttgacag cttatcatcg ataagcttgg tacccaatta
     4381 tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg
     4441 gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac
     4501 attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct
     4561 gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg
     4621 tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct
     4681 gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg
     4741 atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat
     4801 cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag
     4861 gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat
     4921 tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg
     4981 ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag
     5041 caaaatatca ctcggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc
     5101 gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc
     5161 gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta
     5221 tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag
     5281 ag
//
