LOCUS       sequence-250216-        5344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For in vitro translation of shorter human ELL2 from Met1. Also 
            contains Met133I, M1381I, M186I mutations..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C
  TITLE     Transcription elongation factor ELL2 directs immunoglobulin 
            secretion in plasma cells by stimulating altered RNA processing.
  JOURNAL   Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009
            Sep 13.
  PUBMED    19749764
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1786"; 
            journalName: "Nat Immunol"; date: "2009-10"; volume: "10"; issue: 
            "10"; pages: "1102-9"
COMMENT     Sequence Label: 289aa ELL2
FEATURES             Location/Qualifiers
     source          1..5344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(590..601)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(2483..2501)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2522..2538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2522..2538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2535..2557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2546..2562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2570..2576,2577..2594,2595..2600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2615..2636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2753..2770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2924..3512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3004..3023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3683..4474,4475..4543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4306..4325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4544..4648)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4674..5129)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4811..4832)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5023..5042
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5256..5278
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5270..5287
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5271..5287
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5294..5313
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5294..5312
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatggatca ggccaaatcg gccgagctcg
       61 aattcgtcga gagcgggata agttggcgcg tcacctagac gtcagcctct tacctctcgg
      121 acgtcccgtc ccaggagcaa aactctagtg actttgggcc cgcccccgtg agagtggagg
      181 gcggccgatg agggcggttc tgaagggaac aagaccaatg agacgagccc ggggcggggc
      241 gcggggccgg agggtggggc gggtggcggc ggtggcagtc cgaggtctgg ctccgcgcag
      301 tatatgacag tacgtcagca gcgggatggc cctagcagtg gcggcggctg cagaagccca
      361 agcagccgcg gccgcagtgg aggctagagc cggagcggcg gcggcggcgg caccccgggg
      421 agtttaagat ggcggcgggg gggacagggg gcctgcggga ggagcagcgc tatgggctgt
      481 cgtgcggacg gctggggcag gacaacatca ccgtactgca tgtgaagctc accgagacgg
      541 cgatccgggc gctcgagact taccagagcc acaagaattt aattcctttt cgaccttcaa
      601 tccagttcca aggactccac gggcttgtca aaattcccaa aaatgatccc ctcaatgaag
      661 ttcataactt taacttttat ttgtcaaatg tgggcaaaga caaccctcag ggcagctttg
      721 actgcatcca gcaaacattc tccagctctg gagcctccca gctcaattgc ctgggattta
      781 tacaagataa aattacagtg tgtgcaacaa acgactcgta tcagatcaca cgagaaagaa
      841 tcacccaggc agaggaggaa tcccgcaacc gaagcacaaa agttatcaaa cccggtggac
      901 catatgtagg gaaaagagtg caaattcgga aagcacctca agctgtttca gatacagttc
      961 ctgagaggaa aaggtcaacc cccatcaacc ctgcaaatac aattcgaaag acacatagca
     1021 gcagcaccat ctctcagagg ccatacaggg acagggtgat tcacttactg gccctgaagg
     1081 cctacaagaa accggagcta cttgctagac tccagaaaga tggtgtcaat caaaaagaca
     1141 agaactccct gggagcaatt ctgcaacagg tagccaatct gaattctaag gacctctcat
     1201 ataccttaaa ggattatgtt tttaaagagc ttcaaagaga ctggcctgga tacagtgaaa
     1261 tagacagacg gtcattggag tcagtgctct ctagctagaa aactaaatcc gtctcagaat
     1321 gctacaggca ccagccgttc agaatctcct gtatgttcta gtagagatgc tgtatcttct
     1381 cctcagaaac ggcttttgga ttcagagttt attgatcctt taatgaataa aaaagcccga
     1441 atatctcacc tgacgaacag agtaccacca acactaaatg gtcatttgaa tcccaccagt
     1501 gaaaaatcgg ctgcaggcct cccactgccc cctgcggctg ctgccatccc cacccctcca
     1561 ccgctgcctt caacctatct gcccatctca catcctcctc agattgtaaa ttctaactcc
     1621 aactccccta gcactccaga aggccggggg actcaagacc tacctgttga cagttttagt
     1681 caaaacgata gtatctatga ggaccagcaa gacaaatata cctctaggac ttctctggaa
     1741 accttacccc ctggttccgt tctactaaag tgtccaaagc ctatggaaga aaaccattca
     1801 atgtctcaca aaaagtccaa aaagaagtct aaaaaacata aggaaaagga ccaaataaaa
     1861 aagcacgaca ttgagactat tgaggaaaag gaggaagatc ttaagagaga agaggaaatt
     1921 gccaagctaa ataactccag tccaaattcc agtggaggag ttaaagagga ttgcactgcc
     1981 tccatggaac cttcagcaat tgaactccca gattatttga taaaatatat cgctatcgtc
     2041 tcctatgagc aacgccagaa ttataaggat gacttcaatg cagagtatga tgagtacaga
     2101 gctttgcatg ccaggatgga gactgtagct agaagattta tcaaactaga tgcacaaaga
     2161 aagcgccttt ctccaggctc aaaagagtat cagaatgttc atgaagaagt cttacaagaa
     2221 tatcagaaga taaagcagtc tagtcccaat taccatgaag aaaaatacag atgtgaatat
     2281 cttcataaca agctggctca catcaaaagg ctaataggtg aatttgacca acagcaagca
     2341 gagtcatggt cctagaactc tgcttggacc agaagatgtg aataaactta agcttrgaaa
     2401 aaaaaaaaaa aaactctcca gcgctggatc cggccataag ggcctgatcc ttcgaggggg
     2461 ggcccggtac cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat
     2521 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     2581 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     2641 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     2701 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2761 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2821 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2881 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2941 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3001 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3061 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3121 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3181 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3241 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3301 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3361 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3421 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3481 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3541 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3601 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3661 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3721 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3781 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3841 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3901 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3961 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4021 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4081 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4141 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4201 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4261 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4321 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4381 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4441 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4501 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4561 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4621 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     4681 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     4741 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     4801 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     4861 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     4921 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     4981 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     5041 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     5101 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     5161 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     5221 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     5281 ggccagtgaa ttgtaatacg actcactata gggcgaattg gagctccacc gcggtggcgg
     5341 ccgc
//
