LOCUS       sequence-250217-        6263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For in vitro translation of ELL2 with with internal HA tag and 
            Met133, 138, 186 Ileu.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C
  TITLE     Transcription elongation factor ELL2 directs immunoglobulin 
            secretion in plasma cells by stimulating altered RNA processing.
  JOURNAL   Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009
            Sep 13.
  PUBMED    19749764
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1786"; 
            journalName: "Nat Immunol"; date: "2009-10"; volume: "10"; issue: 
            "10"; pages: "1102-9"
COMMENT     Sequence Label: HA tagged Xba ELL2 Met133, 138, 186 to Ileu
FEATURES             Location/Qualifiers
     source          1..6263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(556..567)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1265..1291
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3368..3386)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3407..3423)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3407..3423)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3420..3442)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3431..3447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3455..3461,3462..3479,3480..3485))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3500..3521
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3638..3655)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3809..4397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3889..3908)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4568..5359,5360..5428))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5191..5210
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5429..5533)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5559..6014)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5696..5717)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5908..5927
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6141..6163
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6155..6172
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6156..6172
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6179..6198
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6179..6197
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6230..6263)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggatcaggcc aaatcggccg agctcgaatt cgtcgagagc gggataagtt ggcgcgtcac
       61 ctagacgtca gcctcttacc tctcggacgt cccgtcccag gagcaaaact ctagtgactt
      121 tgggcccgcc cccgtgagag tggagggcgg ccgatgaggg cggttctgaa gggaacaaga
      181 ccaatgagac gagcccgggg cggggcgcgg ggccggaggg tggggcgggt ggcggcggtg
      241 gcagtccgag gtctggctcc gcgcagtata tgacagtacg tcagcagcgg gatggcccta
      301 gcagtggcgg cggctgcaga agcccaagca gccgcggccg cagtggaggc tagagccgga
      361 gcggcggcgg cggcggcacc ccggggagtt taagatggcg gcggggggga cagggggcct
      421 gcgggaggag cagcgctatg ggctgtcgtg cggacggctg gggcaggaca acatcaccgt
      481 actgcatgtg aagctcaccg agacggcgat ccgggcgctc gagacttacc agagccacaa
      541 gaatttaatt ccttttcgac cttcaatcca gttccaagga ctccacgggc ttgtcaaaat
      601 tcccaaaaat gatcccctca atgaagttca taactttaac ttttatttgt caaatgtggg
      661 caaagacaac cctcagggca gctttgactg catccagcaa acattctcca gctctggagc
      721 ctcccagctc aattgcctgg gatttataca agataaaatt acagtgtgtg caacaaacga
      781 ctcgtatcag atcacacgag aaagaatcac ccaggcagag gaggaatccc gcaaccgaag
      841 cacaaaagtt atcaaacccg gtggaccata tgtagggaaa agagtgcaaa ttcggaaagc
      901 acctcaagct gtttcagata cagttcctga gaggaaaagg tcaaccccca tcaaccctgc
      961 aaatacaatt cgaaagacac atagcagcag caccatctct cagaggccat acagggacag
     1021 ggtgattcac ttactggccc tgaaggccta caagaaaccg gagctacttg ctagactcca
     1081 gaaagatggt gtcaatcaaa aagacaagaa ctccctggga gcaattctgc aacaggtagc
     1141 caatctgaat tctaaggacc tctcatatac cttaaaggat tatgttttta aagagcttca
     1201 aagagactgg cctggataca gtgaaataga cagacggtca ttggagtcag tgctctctag
     1261 ggggtaccca tacgacgtcc cagactacgc tgctagaaaa ctaaatccgt ctcagaatgc
     1321 tacaggcacc agccgttcag aatctcctgt atgttctagt agagatgctg tatcttctcc
     1381 tcagaaacgg cttttggatt cagagtttat tgatccttta atgaataaaa aagcccgaat
     1441 atctcacctg acgaacagag taccaccaac actaaatggt catttgaatc ccaccagtga
     1501 aaaatcggct gcaggcctcc cactgccccc tgcggctgct gccatcccca cccctccacc
     1561 gctgccttca acctatctgc ccatctcaca tcctcctcag attgtaaatt ctaactccaa
     1621 ctcccctagc actccagaag gccgggggac tcaagaccta cctgttgaca gttttagtca
     1681 aaacgatagt atctatgagg accagcaaga caaatatacc tctaggactt ctctggaaac
     1741 cttaccccct ggttccgttc tactaaagtg tccaaagcct atggaagaaa accattcaat
     1801 gtctcacaaa aagtccaaaa agaagtctaa aaaacataag gaaaaggacc aaataaaaaa
     1861 gcacgacatt gagactattg aggaaaagga ggaagatctt aagagagaag aggaaattgc
     1921 caagctaaat aactccagtc caaattccag tggaggagtt aaagaggatt gcactgcctc
     1981 catggaacct tcagcaattg aactcccaga ttatttgata aaatatatcg ctatcgtctc
     2041 ctatgagcaa cgccagaatt ataaggatga cttcaatgca gagtatgatg agtacagagc
     2101 tttgcatgcc aggatggaga ctgtagctag aagatttatc aaactagatg cacaaagaaa
     2161 gcgcctttct ccaggctcaa aagagtatca gaatgttcat gaagaagtct tacaagaata
     2221 tcagaagata aagcagtcta gtcccaatta ccatgaagaa aaatacagat gtgaatatct
     2281 tcataacaag ctggctcaca tcaaaaggct aataggtgaa tttgaccaac agcaagcaga
     2341 gtcatggtcc tagaactctg cttggaccag aagatgtgaa taaacttaag cttgraaaaa
     2401 aaaaaaaaaa aaaaaaaaaa aaaaaacgcg taacccccca ttgcaatggt tgccttaagg
     2461 tgtttgctag ttgtgtacat agtgtggtta atcattagct acactgcttc ccacttgatt
     2521 agagcaatgg gaagcatact gtggcctacc agcatctgga agtgtgtgct cgatctgtat
     2581 gtgtgcagag gtggtgtgga tgtgagcgtg catgaaggaa aaaaagctgc tactcctagt
     2641 aggccaaacg ctcaggttaa acaactgacg agtgttactg tagggtgttt ttttgttttt
     2701 ttgttttttt tcttctatca aattgctact tttgttgtgg aagacaaaag catttccatt
     2761 tcaacgagtt tgtcagcttt attaatgttg ggcaaaaatt gatatgtcat gaaaatgaaa
     2821 cagatctata gttttgggac aaaattataa aatgaaatgt gtaggtaacc tatttatata
     2881 ctgctataaa gtattttttg aagagagata tgcaaagaag ctattaccta cataagaggt
     2941 atatttaaag antttttttt ttttcatcct ggtgccagga atataaaaaa gagtggatat
     3001 atttaaccat aacatactgt gattcatcaa acagcacaaa ctttcatttc atggagttta
     3061 tctgttgaca ttgatttaaa ctgtcacttg ttttatcatg tgggaacata agttatgtgg
     3121 tcaaaaatat aaggattttg aattaatgtt gattcaagtt gtattgtctt ttcaaagtgc
     3181 tgccagttga aaagggaagc attatgttta caaatctgtt ttgaaatgtt tgccaaaatt
     3241 ttggtagtgt ctttaataaa gatgtttgtc tccagcatcc agaaaaaaaa aaaaaaaact
     3301 ctccagcgct ggatccggcc ataagggcct gatccttcga gggggggccc ggtaccagct
     3361 tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc
     3421 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3481 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     3541 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     3601 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     3661 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3721 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3781 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3841 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3901 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3961 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4021 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4081 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4141 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4201 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4261 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4321 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4381 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4441 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4501 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4561 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4621 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4681 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4741 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4801 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4861 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4921 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4981 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5041 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5101 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5161 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5221 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5281 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5341 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5401 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5461 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5521 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     5581 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     5641 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     5701 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     5761 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     5821 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     5881 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     5941 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     6001 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     6061 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     6121 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta
     6181 atacgactca ctatagggcg aattggagct ccaccgcggt ggcggccgca taacttcgta
     6241 tagcatacat tatacgaagt tat
//
