LOCUS       sequence-250227-       10854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Global labeling of excitatory synapses (green fluorescence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10854)
  AUTHORS   Bensussen S, Shankar S, Ching KH, Zemel D, Ta TL, Mount RA, Shroff 
            SN, Gritton HJ, Fabris P, Vanbenschoten H, Beck C, Man HY, Han X
  TITLE     A Viral Toolbox of Genetically Encoded Fluorescent Synaptic Tags.
  JOURNAL   iScience. 2020 Jun 30;23(7):101330. doi: 10.1016/j.isci.2020.101330.
  PUBMED    32674057
REFERENCE   2  (bases 1 to 10854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-06-30"; volume: "23(7):101330. doi"; pages: " 
            10.1016/j.isci.2020.101330"
COMMENT     Sequence Label: Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC
FEATURES             Location/Qualifiers
     source          1..10854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    665..898
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1083..1127
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1425..1542
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1587..1598
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     promoter        1607..2895
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             3181..3204
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(3229..3231,3232..3939)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     CDS             join(3229..3231,3232..3939)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(3271..3292)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3532..3551)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3879..3900
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3985..4326
                     /codon_start=1
                     /product="zinc-finger domain from the CCR5-224 (+) 
                     zinc-finger nuclease that recognizes the left half-site 
                     target sequence in the human CCR5 gene (Perez et al., 2008;
                     Gross et al., 2013)"
                     /label=CCR5TC
                     /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN
                     SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH
                     TKIHLRGSQL"
     CDS             4375..4500
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
     misc_feature    4531..5119
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4584..4604)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5002..5013)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5122..5138)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5123..5139)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5644..5824
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5850..5867)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5856..6080
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6126..6554
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6213..6232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6423..6444
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6563..6583)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6568..6897
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6748..6883
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6810..6829
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6945..6992
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7011..7385
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7515..7636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7552..7571)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7606..7625
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7685..7701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7685..7701)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7698..7720)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7709..7725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7733..7739,7740..7757,7758..7763))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7778..7799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7916..7933)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8087..8675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8167..8186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8846..9637,9638..9706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9469..9488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9707..9811)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9886..9905)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10077..10456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10458..10656
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10611..10631
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10674..10854
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
       61 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      121 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      181 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      241 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
      301 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
      361 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
      421 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
      481 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
      541 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
      601 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
      661 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
      721 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
      781 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
      841 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
      901 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
      961 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1021 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1081 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1141 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1201 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     1261 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     1321 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     1381 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     1441 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     1501 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     1561 agcagagatc cagtttggtt aattaagtca tcctcatctt aattaacatt atggccttag
     1621 gtcacttcat ctccatgggg ttcttcttct gattttctag aaaatgagat gggggtgcag
     1681 agagcttcct cagtgacctg cccagggtca catcagaaat gtcagagcta gaacttgaac
     1741 tcagattact aatcttaaat tccatgcctt gggggcatgc aagtacgata tacagaagga
     1801 gtgaactcat tagggcagat gaccaatgag tttaggaaag aagagtccag ggcagggtac
     1861 atctacacca cccgcccagc cctgggtgag tccagccacg ttcacctcat tatagttgcc
     1921 tctctccagt cctaccttga cgggaagcac aagcagaaac tgggacagga gccccaggag
     1981 accaaatctt catggtccct ctgggaggat gggtggggag agctgtggca gaggcctcag
     2041 gaggggccct gctgctcagt ggtgacagat aggggtgaga aagcagacag agtcattccg
     2101 tcagcattct gggtctgttt ggtacttctt ctcacgctaa ggtggcggtg tgatatgcac
     2161 aatggctaaa aagcagggag agctggaaag aaacaaggac agagacagag gccaagtcaa
     2221 ccagaccaat tcccagagga agcaaagaaa ccattacaga gactacaagg gggaagggaa
     2281 ggagagatga attagcttcc cctgtaaacc ttagaaccca gctgttgcca gggcaacggg
     2341 gcaatacctg tctcttcaga ggagatgaag ttgccagggt aactacatcc tgtctttctc
     2401 aaggaccatc ccagaatgtg gcacccacta gccgttacca tagcaactgc ctctttgccc
     2461 cacttaatcc catcccgtct gttaaaaggg ccctatagtt ggaggtgggg gaggtaggaa
     2521 gagcgatgat cacttgtgga ctaagtttgt tcgcatcccc ttctccaacc ccctcagtac
     2581 atcaccctgg gggaacaggg tccacttgct cctgggccca cacagtcctg cagtattgtg
     2641 tatataaggc cagggcaaag aggagcaggt tttaaagtga aaggcaggca ggtgttgggg
     2701 aggcagttac cggggcaacg ggaacagggc gtttcggagg tggttgccat ggggacctgg
     2761 atgctgacga aggctcgcga ggctgtgagc agccacagtg ccctgctcag aagccccaag
     2821 ctcgtcagtc aagccggttc tccgtttgca ctcaggagca cgggcaggcg agtggcccct
     2881 agttctgggg gcagcggggg atccgccacc atgctcgaag tcaaggaagc atcaccaacc
     2941 agcatccaga tcagctgggt gctccacttg cgccacgttc gctactaccg catcacctac
     3001 ggtgaaactg gtggcaatag ccctgtccag gaattcaccg tgcctggcag caagtccact
     3061 gctaccatca gcggcctgaa acctggtgtc gactatacca tcacggtgta cgccgtcacg
     3121 atcttcagcg cctaccgctc cgcctggccg ccgatctcca tcaactaccg caccggaacc
     3181 gattacaagg atgacgacga taagggtagc ggctccagta gatctggggt gagcaagggc
     3241 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3301 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3361 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3421 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3481 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3541 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3601 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3661 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     3721 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     3781 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     3841 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3901 accgccgccg ggatcactct cggcatggac gagctgtact ctaggggagc tggcgctgga
     3961 gcgggtgcag gggctggctc tagattccag tgccggatct gcatgcggaa cttcagcgac
     4021 cggtccaacc tgagcaggca catcagaacc cacaccggag aaaagccctt cgcctgcgac
     4081 atttgcggcc ggaagttcgc catcagcagc aacctgaaca gccacaccaa gatccacact
     4141 ggcagccaga aacctttcca gtgcagaatt tgtatgagaa actttagcag aagcgacaac
     4201 ctggccagac acatccggac acatactggt gaaaaacctt ttgcctgtga tatctgtggc
     4261 agaaagtttg ccacctccgg caatctgacc cggcacacaa agattcacct gcggggcagc
     4321 cagctatcga ttgtcgacgc tcctgaacaa cgtgaaggtg cttctcaagt ttctgtttct
     4381 gttacttttg aagatgttgc tgttcttttt actcgtgatg aatggaaaaa acttgatctt
     4441 tctcaacgtt ctctttatcg tgaagttatg cttgaaaatt attctaatct tgcttctatg
     4501 gcttaagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     4561 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4621 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4681 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4741 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4801 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4861 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4921 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4981 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5041 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5101 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca
     5161 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     5221 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     5281 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     5341 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     5401 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     5461 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     5521 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     5581 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
     5641 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5701 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     5761 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5821 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5881 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5941 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     6001 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     6061 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     6121 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6181 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6241 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6301 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6361 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6421 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6481 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6541 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     6601 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     6661 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6721 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6781 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     6841 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     6901 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     6961 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     7021 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     7081 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     7141 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     7201 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     7261 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     7321 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     7381 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     7441 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     7501 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7561 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7621 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     7681 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7741 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7801 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7861 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7921 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7981 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8041 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8101 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8161 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8221 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8281 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8341 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8401 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8461 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8521 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8581 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8641 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8701 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8761 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8821 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8881 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8941 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9001 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9061 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9121 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9181 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9241 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9301 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9361 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9421 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9481 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9541 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9601 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9661 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9721 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9781 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9841 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     9901 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     9961 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
    10021 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
    10081 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
    10141 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
    10201 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
    10261 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
    10321 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
    10381 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
    10441 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
    10501 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
    10561 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
    10621 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
    10681 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    10741 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    10801 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agca
//
