LOCUS       sequence-250229-        7078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Exogenous expression of PSD95 (red fluorescence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Bensussen S, Shankar S, Ching KH, Zemel D, Ta TL, Mount RA, Shroff 
            SN, Gritton HJ, Fabris P, Vanbenschoten H, Beck C, Man HY, Han X
  TITLE     A Viral Toolbox of Genetically Encoded Fluorescent Synaptic Tags.
  JOURNAL   iScience. 2020 Jun 30;23(7):101330. doi: 10.1016/j.isci.2020.101330.
  PUBMED    32674057
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-06-30"; volume: "23(7):101330. doi"; pages: " 
            10.1016/j.isci.2020.101330"
COMMENT     Sequence Label: AAV-Syn-PSD95-mCherry
FEATURES             Location/Qualifiers
     source          1..7078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..39
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     promoter        67..514
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      542..551
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             584..613
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2774..3484
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2919..2937)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3187..3206
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3497..4085
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3550..3570)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3968..3979)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4088..4104)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4089..4105)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    4134..4255
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4145..4164)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4199..4218
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4634..5222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4714..4733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5393..6184,6185..6253))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6016..6035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6254..6358)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6384..6839)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6521..6542)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6733..6752
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   6911..7040
                     /label=AAV2 ITR
ORIGIN
        1 tagccatgct ctaggaagat cacgcgtgtc atcctcatca gatctctgca gagggccctg
       61 cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac
      121 gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct
      181 atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc
      241 accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga
      301 aggcgcgctg acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg
      361 cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca
      421 cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg
      481 cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaat tcaagctgct agcaaggatc
      541 cgccaccatg gactgtctct gtatagtgac aacccggcgc gccgagcaaa agctgatatc
      601 tgaagaggac ttggggcgcg ccaagaaata ccgctaccaa gatgaagaca cgccccctct
      661 ggaacacagc ccggcccacc tccccaacca ggccaattct ccccctgtga ttgtcaacac
      721 ggacacccta gaagccccag gatatgagtt gcaggtgaat ggaacagagg gggagatgga
      781 gtatgaggag atcacattgg aaaggggtaa ctcaggtctg ggcttcagca tcgcaggtgg
      841 cactgacaac ccgcacatcg gtgacgaccc gtccattttt atcaccaaga tcattcctgg
      901 tggggctgca gcccaggatg gccgcctcag ggtcaatgac agcatcctgt ttgtaaatga
      961 agtggatgtt cgggaggtga cccattcagc tgcggtggag gccctcaaag aggcaggttc
     1021 catcgttcgc cactatgtca tgcgccggaa acccccagcc gaaaaggtca tggagatcaa
     1081 actcatcaaa gggcctaaag gacttggctt cagcattgcg gggggcgttg ggaaccagca
     1141 catccctgga gataacagca tctatgtaac gaagatcatc gaaggaggtg ctgctcacaa
     1201 ggatggcagg ttgcagattg gagacaagat cctggcggtc aacagtgtgg ggctggagga
     1261 cgttatgcac gaggatgccg tggcagccct gaagaacaca tatgacgttg tgtacctaaa
     1321 ggtggccaag cccagcaatg cctacctgag tgacagctat gctcccccag acatcacaac
     1381 ctcgtattct cagcacctgg acaatgagat cagtcatagc agctacttgg gcactgacta
     1441 ccccacagcc atgaccccca cttcccctcg gcgctactcc cctgtggcca aggacctgct
     1501 gggggaggaa gacattcccc gggaaccaag gcggatcgtg atccatcggg gctccaccgg
     1561 cctgggcttc aacatcgtgg gcggcgagga tggtgaaggc atcttcatct ccttcatcct
     1621 tgctgggggt ccagccgacc tcagtgggga gctacggaag ggggaccaga tcctgtcggt
     1681 caatggtgtt gacctccgca atgccagtca cgaacaggct gccattgccc tgaagaatgc
     1741 gggtcagacg gtcacgatca tcgctcagta taaaccagaa gagtatagtc gattcgaggc
     1801 caagatccat gatcttcggg aacagctcat gaatagtagc ctaggctcag ggactgcatc
     1861 cttgcgaagc aaccccaaga ggggcttcta cattagggcc ctgtttgatt acgacaagac
     1921 caaggactgc ggtttcttga gccaggccct gagcttccgc ttcggggatg tgcttcatgt
     1981 cattgacgtt ggtgacgaag agtggtggca agcacggcgg gtccactccg acagtgagac
     2041 cgacgacatt ggcttcattc ccagcaaacg gcgggtcgag cgacgagagt ggtcaaggtt
     2101 aaaggccaag gactggggct ccagctctgg atcacagggt cgagaagact cggttctgag
     2161 ctatgagacg gtgacccaga tggaagtgca ctatgctcgt cccatcatca tccttggacc
     2221 caccaaagac cgtgccaacg atgatcttct ctccgagttc cccgacaagt ttggatcctg
     2281 tgtccctcat acgacacgtc ctaagcggga atatgagata gacggccagg attaccactt
     2341 tgtctcctcc cgggagaaaa tggagaagga catccaggca cacaagttca ttgaggctgg
     2401 ccagtacaac agccacctct atgggaccag cgtccagtct gtgcgagagg tagcagagca
     2461 ggggaagcac tgcatcctcg atgtctcggc caatgccgtg cggcggctgc aggcggccca
     2521 cctgcacccc atcgccatct tcatccgtcc ccgctccctg gagaatgtgc tagagatcaa
     2581 taagcggatc acagaggagc aagcccggaa agccttcgac agagccacga agctggagca
     2641 ggagttcaca gagtgcttct cagccatcgt agagggcgac agctttgaag agatctatca
     2701 caaagtgaaa cgtgtcattg aagacctctc aggcccctac atctgggtcc cagcccgaga
     2761 gagactcgaa ttcatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     2821 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     2881 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     2941 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     3001 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     3061 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     3121 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     3181 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     3241 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     3301 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     3361 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     3421 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     3481 gtaaaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3541 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3601 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3661 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3721 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3781 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3841 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     3901 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3961 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4021 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4081 cccgcatcga taccgtcgac ctcgacccgg gcggccgctt cgagcagaca tgataagata
     4141 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     4201 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     4261 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
     4321 caagtaaaac ctctacaaat gtggtaaaat cgataaggat cttcctagag catggctacg
     4381 tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg
     4441 ccactccctc tctgcgcgct csctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4501 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4561 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4621 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4681 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4741 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4801 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4861 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4921 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4981 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5041 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5101 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5161 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5221 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5281 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5341 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5401 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5461 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5521 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5581 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5641 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5701 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5761 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5821 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5881 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5941 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6001 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6061 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     6121 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     6181 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6241 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6301 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6361 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     6421 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     6481 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     6541 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     6601 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     6661 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     6721 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     6781 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     6841 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     6901 attacgccag ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg
     6961 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa
     7021 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctacg
//
