LOCUS       sequence-25029-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Venken KJ, Kasprowicz J, Kuenen S, Yan J, Hassan BA, Verstreken P
  TITLE     Recombineering-mediated tagging of Drosophila genomic constructs for
            in vivo localization and acute protein inactivation.
  JOURNAL   Nucleic Acids Res. 2008 Oct;36(18):e114. doi: 10.1093/nar/gkn486. 
            Epub 2008 Aug 1.
  PUBMED    18676454
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2008-10"; volume: "36(18):e114. doi"; pages: " 
            10.1093/nar/gkn486. Epub 2008 Aug 1"
COMMENT     Sequence Label: PL-452 N-YPet
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(82..795)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
ORIGIN
        1 cgcctcccct acccggtaga atttcgacga cctgcagcca agctagcata acttcgtata
       61 gcatacatta tacgaagtta ttttgtacaa ttcattcata ccctcggtaa taccagcagc
      121 agtcaaaaat tctaacaaga ccatgtggtc tctcttttcg tttggatctt tgaataaggc
      181 agattgatag gataagtaat ggttgtctgg taacaagact ggaccatcac caattggagt
      241 attttgttga taatggtcag ctaattgaac accaccatct tcaatgttgt gtctaatttt
      301 gaagttagct ttgataccat tcttttgttt gtcagcagtg atgtaaacat tgtgagagtt
      361 atagttgtat tccaatttgt gacctaaaat gttaccatct tctttaaaat caataccttt
      421 taattcgatt ctattaacta aggtatcacc ttcaaacttg acttcagctc tggtcttgta
      481 gttaccgtca tctttgaaaa aaatagttct ttcttgaaca taaccttctg gcatggcaga
      541 cttgaaaaag tcatgttgtt tcatatgatc tgggtatcta gcaaaacatt gaacaccata
      601 acctaaagta gtgactaagg ttggccatgg aactggcaat ttaccagtag tacagagtaa
      661 ttttaaggtc aatttaccgt acgtagcatc accttcacct tcaccggaga cagaaaattt
      721 gtgaccatta acatcaccat ctaattcaac caaaattggg acaacaccag tgaataattc
      781 ttcaccttta gacatggnac cagcttttgt tcccttta
//
