LOCUS       sequence-25031-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Venken KJ, Kasprowicz J, Kuenen S, Yan J, Hassan BA, Verstreken P
  TITLE     Recombineering-mediated tagging of Drosophila genomic constructs for
            in vivo localization and acute protein inactivation.
  JOURNAL   Nucleic Acids Res. 2008 Oct;36(18):e114. doi: 10.1093/nar/gkn486. 
            Epub 2008 Aug 1.
  PUBMED    18676454
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2008-10"; volume: "36(18):e114. doi"; pages: " 
            10.1093/nar/gkn486. Epub 2008 Aug 1"
COMMENT     Sequence Label: PL-452 N-mDsRed
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..80)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(81..755)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNXEDVIKEFMQXKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     complement(338..357)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgccncncnn cccggtagaa tttcgacgac ctgcagccaa gctagcataa cttcgtatag
       61 catacattat acgaagttat ctgggagccg gagtggcggg cctcggcgtg ctcgtactgc
      121 tccaccacgg tgtagtcctc gttgtggttg gtgatgtcca gcttggagtc cacgtagtgg
      181 ttgccgggca gctgcacggg cttcttggcc ttgtacacgg tcttgaagtc gcaggtgtag
      241 tggccgccgt ccttcagctt cagggcgtgg gagatctcgc ccttcagcac gccgtcctgg
      301 gggtacagct tctcggtgga gggctcccag ccggcagtct tcttctgcat tacggggccg
      361 tcggcgggga agttcacgcc cttgaacttc accttgtaga tgaaggtgcc gtcctgcagg
      421 gaggagtcct gctgcacctc caccacgccg ccgtcctcga agttcatgga gcgctcccag
      481 gtgaagccct cggggaagga cagcttcatg tagtcgggga tgtcggcggg gtgcttcacg
      541 taggccttgg agccgtactg gaactggggg gacaggatgt cccaggcgaa gggcaggggg
      601 ccgcccttgg tcacctgcag cttggcggtc tgggtgccct cgtagggctt gccctcgccc
      661 tcgccctcga tctcgaagta gtggccgttc acggagccct ccatgcgcac cttgnnctgc
      721 atgaactcct tgatgacgtc ctcngngttg tccatggtac cagcttttgt tccctttagt
      781 gagggtta
//
