LOCUS       sequence-250320-         372 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses His-tagged FAST (also called YFAST) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Plamont MA, Billon-Denis E, Maurin S, Gauron C, Pimenta FM, Specht 
            CG, Shi J, Querard J, Pan B, Rossignol J, Moncoq K, Morellet N, 
            Volovitch M, Lescop E, Chen Y, Triller A, Vriz S, Le Saux T, Jullien
            L, Gautier A
  TITLE     Small fluorescence-activating and absorption-shifting tag for 
            tunable protein imaging in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):497-502. doi: 
            10.1073/pnas.1513094113. Epub 2015 Dec 28.
  PUBMED    26711992
REFERENCE   2  (bases 1 to 372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1513094113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-01-19- 19"; volume: "113"; issue: "3"; pages: "497-502"
COMMENT     Sequence Label: pAG87 FAST
FEATURES             Location/Qualifiers
     source          1..372
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..372
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="EHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAAE
                     GDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTKV
                     KVHMKKALSGDSYWVFVKRV"
ORIGIN
        1 gaacatgttg cctttggtag cgaagatatc gagaatactc tagcgaaaat ggatgatggc
       61 caattagatg gattagcctt tggtgctata cagcttgatg gtgatggcaa tattctgcag
      121 tataatgcag ctgaaggaga cataacaggg agagatccca aacaagtgat tggcaagaac
      181 ttcttcaaag acgtagcacc aggtacagat tctcctgaat tttacgggaa attcaaggaa
      241 ggagtagcat caggtaactt gaataccatg ttcgagtgga tgattccgac gagtaggggg
      301 ccaaccaagg ttaaagtcca catgaagaag gctttgagtg gtgactccta ttgggtgttt
      361 gtcaaaaggg tt
//
