LOCUS       sequence-250329-         375 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FAST (also called YFAST) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Plamont MA, Billon-Denis E, Maurin S, Gauron C, Pimenta FM, Specht 
            CG, Shi J, Querard J, Pan B, Rossignol J, Moncoq K, Morellet N, 
            Volovitch M, Lescop E, Chen Y, Triller A, Vriz S, Le Saux T, Jullien
            L, Gautier A
  TITLE     Small fluorescence-activating and absorption-shifting tag for 
            tunable protein imaging in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):497-502. doi: 
            10.1073/pnas.1513094113. Epub 2015 Dec 28.
  PUBMED    26711992
REFERENCE   2  (bases 1 to 375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1513094113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-01-19- 19"; volume: "113"; issue: "3"; pages: "497-502"
COMMENT     Sequence Label: pAG104 FAST
FEATURES             Location/Qualifiers
     source          1..375
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..375
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKVHMKKALSGDSYWVFVKRV"
ORIGIN
        1 atggagcatg ttgcctttgg cagtgaggac atcgagaaca ctctggccaa aatggacgac
       61 ggacaactgg atgggttggc ctttggcgca attcagctcg atggtgacgg gaatatcctg
      121 cagtacaatg ctgctgaagg agacatcaca ggcagagatc ccaaacaggt gattgggaag
      181 aacttcttca aggatgttgc acctggaacg gattctcccg agttttacgg caaattcaag
      241 gaaggcgtag cgtcagggaa tctgaacacc atgttcgaat ggatgatacc gacaagcagg
      301 ggaccaacca aggtcaaggt gcacatgaag aaagcccttt ccggtgacag ctattgggtc
      361 tttgtgaaac gggtg
//
