LOCUS       sequence-250332-         420 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FAST (also called YFAST) fused to Lyn11 sequence in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Plamont MA, Billon-Denis E, Maurin S, Gauron C, Pimenta FM, Specht 
            CG, Shi J, Querard J, Pan B, Rossignol J, Moncoq K, Morellet N, 
            Volovitch M, Lescop E, Chen Y, Triller A, Vriz S, Le Saux T, Jullien
            L, Gautier A
  TITLE     Small fluorescence-activating and absorption-shifting tag for 
            tunable protein imaging in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):497-502. doi: 
            10.1073/pnas.1513094113. Epub 2015 Dec 28.
  PUBMED    26711992
REFERENCE   2  (bases 1 to 420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1513094113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-01-19- 19"; volume: "113"; issue: "3"; pages: "497-502"
COMMENT     Sequence Label: pAG106 Lyn11-FAST
FEATURES             Location/Qualifiers
     source          1..420
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..420
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="EHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAAE
                     GDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTKV
                     KVHMKKALSGDSYWVFVKRV"
ORIGIN
        1 atgggctgca tcaagtccaa gggcaaggac tccgccggcg gcggctccga gcatgttgcc
       61 tttggcagtg aggacatcga gaacactctg gccaaaatgg acgacggaca actggatggg
      121 ttggcctttg gcgcaattca gctcgatggt gacgggaata tcctgcagta caatgctgct
      181 gaaggagaca tcacaggcag agatcccaaa caggtgattg ggaagaactt cttcaaggat
      241 gttgcacctg gaacggattc tcccgagttt tacggcaaat tcaaggaagg cgtagcgtca
      301 gggaatctga acaccatgtt cgaatggatg ataccgacaa gcaggggacc aaccaaggtc
      361 aaggtgcaca tgaagaaagc cctttccggt gacagctatt gggtctttgt gaaacgggtg
//
