LOCUS       sequence-250337-         474 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FAST (also called YFAST) fused to a mitochondria targeting
            sequence (mito) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Plamont MA, Billon-Denis E, Maurin S, Gauron C, Pimenta FM, Specht 
            CG, Shi J, Querard J, Pan B, Rossignol J, Moncoq K, Morellet N, 
            Volovitch M, Lescop E, Chen Y, Triller A, Vriz S, Le Saux T, Jullien
            L, Gautier A
  TITLE     Small fluorescence-activating and absorption-shifting tag for 
            tunable protein imaging in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):497-502. doi: 
            10.1073/pnas.1513094113. Epub 2015 Dec 28.
  PUBMED    26711992
REFERENCE   2  (bases 1 to 474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1513094113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-01-19- 19"; volume: "113"; issue: "3"; pages: "497-502"
COMMENT     Sequence Label: pAG156 Mito-FAST
FEATURES             Location/Qualifiers
     source          1..474
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..75
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      94..103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..474
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKVHMKKALSGDSYWVFVKRV"
ORIGIN
        1 atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca
       61 gtgccgcgcg ccaagatcca ttcgttgaga tctgccacca tggagcatgt tgcctttggc
      121 agtgaggaca tcgagaacac tctggccaaa atggacgacg gacaactgga tgggttggcc
      181 tttggcgcaa ttcagctcga tggtgacggg aatatcctgc agtacaatgc tgctgaagga
      241 gacatcacag gcagagatcc caaacaggtg attgggaaga acttcttcaa ggatgttgca
      301 cctggaacgg attctcccga gttttacggc aaattcaagg aaggcgtagc gtcagggaat
      361 ctgaacacca tgttcgaatg gatgataccg acaagcaggg gaccaaccaa ggtcaaggtg
      421 cacatgaaga aagccctttc cggtgacagc tattgggtct ttgtgaaacg ggtg
//
