LOCUS       sequence-25034-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Venken KJ, Kasprowicz J, Kuenen S, Yan J, Hassan BA, Verstreken P
  TITLE     Recombineering-mediated tagging of Drosophila genomic constructs for
            in vivo localization and acute protein inactivation.
  JOURNAL   Nucleic Acids Res. 2008 Oct;36(18):e114. doi: 10.1093/nar/gkn486. 
            Epub 2008 Aug 1.
  PUBMED    18676454
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2008-10"; volume: "36(18):e114. doi"; pages: " 
            10.1093/nar/gkn486. Epub 2008 Aug 1"
COMMENT     Sequence Label: PL-452 N-Flag-4C
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        31..49
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             70..93
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             118..135
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     protein_bind    145..178
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        212..711
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(247..270)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     644..663
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        723..770
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(852..871)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cagctatgac catgattacg ccaagctcga aattaaccct cactaaaggg aacaaaagct
       61 ggtaccatgg attacaagga tgacgacgat aagggctccg ccggatcgtt tttgaattgc
      121 tgccccggct gctgcatgga gcccataact tcgtataatg tatgctatac gaagttatgc
      181 tagcttggct gcaggtcgtc gaaattctac cgggtagggg aggcgctttt cccaaggcag
      241 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
      301 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
      361 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
      421 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
      481 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
      541 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
      601 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
      661 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
      721 ctgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
      781 actaaaccat gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt
      841 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
      901 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccc
      961 gtgcctgatg actgcagaca g
//
