LOCUS       sequence-250344-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses td-FAST in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Tebo AG, Pimenta FM, Zhang Y, Gautier A
  TITLE     Improved Chemical-Genetic Fluorescent Markers for Live Cell 
            Microscopy.
  JOURNAL   Biochemistry. 2018 Oct 2;57(39):5648-5653. doi: 
            10.1021/acs.biochem.8b00649. Epub 2018 Sep 17.
  PUBMED    30204425
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.8b00649"; journalName: "Biochemistry"; date: 
            "2018-10-2- 2"; volume: "57"; issue: "39"; pages: "5648-5653"
COMMENT     Sequence Label: pAG330 td-FAST
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..375
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKVHMKKALSGDSYWVFVKRV"
     CDS             397..768
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="EHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAAE
                     GDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTKV
                     KVHMKKALSGDSYWVFVKRV"
ORIGIN
        1 atggaacatg tcgccttcgg cagcgaggat atcgagaaca ctctggctaa gatggatgac
       61 gggcaactgg acggcttggc attcggggcc attcaactgg acggggacgg taatattctc
      121 cagtacaatg cagctgaagg tgacataact ggacgtgatc ctaaacaagt gattggcaag
      181 aatttcttca aagatgttgc tcccggcacc gattcacctg agttttacgg gaagttcaaa
      241 gagggcgttg cctccggcaa cctcaacact atgttcgaat ggatgatacc gacaagcaga
      301 ggcccaacca aggtaaaagt tcatatgaag aaagctctgt ctggagactc ttattgggtg
      361 tttgtgaaga gagtcggcgg cggctccgga ggaggagaac acgttgcctt tggctctgag
      421 gatatcgaaa atacgctggc caaaatggat gacggtcagc tggatggcct ggcattcgga
      481 gccatacagc ttgacggcga tggaaacatc ctccagtaca acgccgcgga gggtgacatt
      541 accggtcgcg atccaaaaca ggttattggc aagaacttct ttaaggacgt tgcacccggg
      601 actgactctc ccgaatttta cggaaagttc aaggaagggg tggcatctgg caatcttaat
      661 acaatgtttg aatggatgat tcctactagt agaggaccaa caaaggtgaa ggtgcacatg
      721 aagaaagcct tgtccggcga ctcctactgg gtctttgtga aacgggtg
//
