LOCUS       sequence-250348-        9798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the 2nd gen. Lenti Virus GFP dropout destination vector 
            without insulator as a type 0 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 9798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK0_062
FEATURES             Location/Qualifiers
     source          1..9798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             251..262
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             269..982
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      994..1065
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1081..1108
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1388..1976
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1441..1461)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1859..1870)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2039..2272
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2297..2315)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2298..2315)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2605..2624)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2838..2857)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2957..2979
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3017..3035)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(3214..3802)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3294..3313)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(4016..4035)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4027..4842
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             4027..4842
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4100..4119)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5017..5034
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5104..5124)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5109..5438
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5289..5424
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6572..6637
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6767..6787
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7212..7346
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7249..7268)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7303..7322
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7514..8147
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8194..8319
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8816..9049
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9234..9278
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9563..9680
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcatgcgcgg atgacacgaa ctcacgacgg cgatcacaga cattaaccca cagtacagac
       61 actgcgacaa cgtggcaatt cgtcgcaata caacgtgaga ccgaaagtga aacgtgattt
      121 catgcgtcat tttgaacatt ttgtaaatct tatttaataa tgtgtgcggc aattcacatt
      181 taatttatga atgttttctt aacatcgcgg caactcaaga aacggcaggt tcggatctta
      241 gctactagag aaagaggaga aatactagat gcgtaaaggc gaagagctgt tcactggtgt
      301 cgtccctatt ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg
      361 cgagggtgaa ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg
      421 taaactgccg gttccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt
      481 tgctcgttat ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg
      541 ctatgtgcag gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga
      601 agtgaaattt gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa
      661 agaggacggc aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta
      721 catcaccgcc gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt
      781 ggaggatggc agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg
      841 tcctgttctg ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc
      901 gaacgagaaa cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca
      961 tggtatggat gaactgtaca aatgaccagg catcaaataa aacgaaaggc tcagtcgaaa
     1021 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1081 ggctcacctt cgggtgggcc tttctgcgtt tataggtctc agctggaaat ctgctcgtca
     1141 gtggtgctca cactgacgaa tcatgtacag atcataccga tgactgcctg gcgactcaca
     1201 actaagcaag acagccggaa ccagcgccgg cgaacaccac tgcatatatg gcatatcaca
     1261 acagtccacg tctcaagcag ttacagagat gttacgaacc actagtgcac tgcagtacat
     1321 gactgactga ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct
     1381 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1441 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1501 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1561 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     1621 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     1681 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     1741 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     1801 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     1861 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     1921 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatca
     1981 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     2041 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtttctc
     2101 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     2161 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     2221 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
     2281 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
     2341 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
     2401 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
     2461 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
     2521 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     2581 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     2641 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     2701 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     2761 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     2821 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     2881 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     2941 caagctgtga ccatctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     3001 cgcgcgagat gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3061 atggtttctt agacgtcagg tggcactttt cggggaaatg tgccgagcgg ccgcacggtt
     3121 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3181 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3661 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3721 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3781 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3841 gtggaacgaa aactcacgtt aagggatttt ggtcatgact gatccttcaa ctcagcaaaa
     3901 gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa
     3961 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     4021 ggtgttatga gccatattca acgggaaacg tcttgctcca ggccgcgatt aaattccaac
     4081 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     4141 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     4201 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     4261 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     4321 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     4381 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     4441 tgtcctttta acagcgatcg cgtatttcgc ctcgctcagg cgcaatcacg aatgaataac
     4501 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     4561 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     4621 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     4681 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     4741 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     4801 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     4861 ttgtaacacg cggccgccaa ttttttacgg ttcctggcct tttgctggcc ttttgctcac
     4921 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4981 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     5041 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     5101 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5161 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5221 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5281 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5341 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     5401 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
     5461 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     5521 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     5581 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     5641 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     5701 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac
     5761 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     5821 gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg
     5881 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     5941 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     6001 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     6061 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     6121 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     6181 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     6241 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     6301 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     6361 cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc
     6421 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt
     6481 tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     6541 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     6601 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     6661 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     6721 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     6781 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     6841 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     6901 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     6961 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     7021 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     7081 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     7141 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     7201 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7261 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7321 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     7381 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     7441 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     7501 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
     7561 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     7621 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     7681 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     7741 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     7801 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     7861 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     7921 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactggga ctctctggtt
     7981 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8041 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8101 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     8161 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     8221 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     8281 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     8341 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     8401 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     8461 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8521 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     8581 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8641 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     8701 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     8761 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     8821 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     8881 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     8941 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     9001 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     9061 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     9121 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     9181 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     9241 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     9301 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     9361 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     9421 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     9481 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
     9541 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     9601 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9661 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggccct
     9721 gaattcgcat ctagactgat gagacgtggt agagccacaa acagccggta caagcaacga
     9781 tctccaggac catctgaa
//
