LOCUS       sequence-250387-        4566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW
  TITLE     Identification and characterization of genes encoding the nuclear 
            envelope LINC complex in the monocot species Zea mays.
  JOURNAL   J Cell Sci. 2019 Feb 11;132(3). pii: jcs.221390. doi: 
            10.1242/jcs.221390.
  PUBMED    30659121
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-02-11"; volume: "132(3). pii: jcs.221390. doi"; 
            pages: " 10.1242/jcs.221390"
COMMENT     Sequence Label: SUN2delCCec
FEATURES             Location/Qualifiers
     source          1..4566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1388..1411
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1415..1441
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2691..2790)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2807..2826)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2808..2826)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2831..2847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2831..2847)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2949..2968)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2960..3769
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3027..3046)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3916..4504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4405..4424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc atggtgtcca agggcgagga ggacaacatg gccatcatta aggagttcat
      721 gcgcttcaag gtccacatgg agggctccgt taatgggcat gagttcgaga tcgaggggga
      781 gggcgagggg aggccctacg agggcacaca gactgccaag ctgaaggtga cgaagggcgg
      841 gcctctcccc ttcgcctggg acatcctgtc gcctcagttc atgtacggct ctaaggccta
      901 cgtgaagcac cccgctgaca ttccagatta cctcaagctg agcttcccgg agggcttcaa
      961 gtgggagcgg gttatgaact tcgaggacgg cggggtggtc acggtgaccc aggactccag
     1021 cctccaggat ggcgagttca tctacaaggt caagctgcgc ggcacaaatt tcccttccga
     1081 cgggccggtt atgcagaaga agactatggg ctgggaggcc tcgtctgagc ggatgtaccc
     1141 ggaggatggc gcgctcaagg gggagattaa gcagcgcctc aagctgaagg acggtggcca
     1201 ctacgatgct gaggtgaaga ccacgtacaa ggccaagaag ccggtccagc tcccaggcgc
     1261 ctacaacgtt aatatcaagc tggacattac atcgcacaac gaggattaca ctatcgtgga
     1321 gcagtacgag agggccgagg gcaggcattc tacgggcggg atggacgagc tgtacaaggg
     1381 cgccggggat tacaaggacg atgacgataa ggcgtaccct tacgacgtcc ccgattacgc
     1441 gggcgccggg atggcttctc cctcattcgc ggccgctgct acctctccgg cgtcatcccc
     1501 actcacgctg gacgctatcc cactggcctc aaggcccgcc ccaggcgccg ctgcggccca
     1561 gcggaagcgc tcggtgttcc tcctgaacca ccggcctcat ccggtctcgc ccaccccgcc
     1621 cctgcagcca accgctgcgg cctccgctca gcacgccagg aggaagaaga cctctcatcc
     1681 acctaggccc aggtggcaga ccgtgctctc catcgctgcc aagaatgccg ctctcctggc
     1741 ggccctcctg tacctcggcg acctggcctg gaggtggagc cacccgcccc cacctagccc
     1801 gcccccagac cgggctgccc tggagggcta cgcggctcgc gtcgatgagg ttgaggccag
     1861 cctcacacgg actttccgca tgatccaggt ccagctggag gcggttgaca ggaagattga
     1921 tggcgaagtg ggggcggcgc ggggcgggga tgcgctcgct ggcctgaaga ggatggagtt
     1981 cctccggaag gacgagttcg agaagttctg ggatgaggtg aagggctcac tgggctcctc
     2041 ctccgagtcg gaggtggacc tcgatcaggt ccgggccctg gcgcgcgaga tcgtcatgcg
     2101 cgagattgag aagcacgctg cggacggcat cgggcgggtg gattacgctg tggcttccgg
     2161 cgggggcagg gttgtgcggc atagcgaggc ctacgttccc aagaggggct tcatggtgtg
     2221 gatgtccggc gtggacgttg ggcccaagcc agagaagatg ctgcagccta gcttcggcga
     2281 gcccgggcag tgcttcgctc tccagggctc aaacgggttc gtggaggtca agctgaagtc
     2341 cggcatcatt ccagaggcgg tgaccctgga gcacgtttct aaggacgtgg cctacgatcg
     2401 ctcaacggcg cctaagggct gcagggtcta cgggtggtac gaggagacgc ccggcgagac
     2461 ccagtcgggg catgccgcta agatggcgct cgctgagttc gcctacgacc tggagaagaa
     2521 caatgttcag acattcgacg tgactgcgcc ggatgttggc gtgatcaaca tggtgcgcct
     2581 cgacttcaca tcgaatcacg gctcatccca gctcacttgc atctaccgcc tgcgggtgca
     2641 cggccatgag cctgtcagcc ccggcagctc ggctgggtcg caggcctgag acccagcttt
     2701 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2761 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2821 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     2881 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     2941 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     3001 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     3061 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     3121 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     3181 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     3241 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     3301 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     3361 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     3421 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     3481 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     3541 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3601 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3661 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     3721 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     3781 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     3841 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     3901 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3961 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4021 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4081 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4141 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4201 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4261 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4321 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4381 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4441 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4501 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4561 catgtt
//
