LOCUS       sequence-250389-        6063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Leach FS, Nicolaides NC, Papadopoulos N, Liu B, Jen J, Parsons R, 
            Peltomaki P, Sistonen P, Aaltonen LA, Nystroem-Lahti M
  TITLE     Mutations of a mutS homolog in hereditary nonpolyposis colorectal 
            cancer.
  JOURNAL   Cell. 1993 Dec 17. 75(6):1215-25.
  PUBMED    8261515
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1993 
            Dec 17. 75(6):1215-25."
COMMENT     Sequence Label: hMSH2 Clone E3 (full-length)
FEATURES             Location/Qualifiers
     source          1..6063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(20..36)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(21..37)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(61..80)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(62..80)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(87..104)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(87..103)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(96..118)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     614..633
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        747..851
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(852..920,921..1712)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1070..1089)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1883..2471
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2372..2391
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2625..2642
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2759..2780
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2795..2800,2801..2818,2819..2825)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2833..2849
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2838..2860
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2857..2873
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2857..2873
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2894..2912
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aattcgatat caagcttatc gataccgtcg acctcgaggg ggggcccggt acccaattcg
       61 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
      121 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      181 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      241 tggcaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
      301 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
      361 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactatttgg aacaagagtc
      421 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
      481 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
      541 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
      601 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
      661 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
      721 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      781 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      841 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      901 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      961 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1021 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1081 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1141 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1201 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1261 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1321 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1381 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1441 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1501 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1561 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1621 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1681 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1741 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1801 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1861 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1921 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1981 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2041 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2101 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2161 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2221 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2281 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2341 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2401 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2461 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2521 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2581 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2641 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2701 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2761 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2821 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2881 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg
     2941 gcggccgctc tagaggaggt ttcgccatgg cggtgcagcc gaaggagacg ctgcagttgg
     3001 agagcgcggc cgaggtcggc ttcgtgcgct tctttcaggg catgccggag aagccgacca
     3061 ccacagtgcg ccttttcgac cggggcgact tctatacggc gcacggcgag gacgcgctgc
     3121 tggccgcccg ggaggtgttc aagacccagg gggtgatcaa gtacatgggg ccggcaggag
     3181 caaagaatct gcagagtgtt gtgcttagta aaatgaattt tgaatctttt gtaaaagatc
     3241 ttcttctggt tcgtcagtat agagttgaag tttataagaa tagagctgga aataaggcat
     3301 ccaaggagaa tgattggtat ttggcatata aggcttctcc tggcaatctc tctcagtttg
     3361 aagacattct ctttggtaac aatgatatgt cagcttccat tggtgttgtg ggtgttaaaa
     3421 tgtccgcagt tgatggccag agacaggttg gagttgggta tgtggattcc atacagagga
     3481 aactaggact gtgtgaattc cctgataatg atcagttctc caatcttgag gctctcctca
     3541 tccagattgg accaaaggaa tgtgttttac ccggaggaga gactgctgga gacatgggga
     3601 aactgagaca gataattcaa agaggaggaa ttctgatcac agaaagaaaa aaagctgact
     3661 tttccacaaa agacatttat caggacctca accggttgtt gaaaggcaaa aagggagagc
     3721 agatgaatag tgctgtattg ccagaaatgg agaatcaggt tgcagtttca tcactgtctg
     3781 cggtaatcaa gtttttagaa ctcttatcag atgattccaa ctttggacag tttgaactga
     3841 ctacttttga cttcagccag tatatgaaat tggatattgc agcagtcaga gcccttaacc
     3901 tttttcaggg ttctgttgaa gataccactg gctctcagtc tctggctgcc ttgctgaata
     3961 agtgtaaaac ccctcaagga caaagacttg ttaaccagtg gattaagcag cctctcatgg
     4021 ataagaacag aatagaggag agattgaatt tagtggaagc ttttgtagaa gatgcagaat
     4081 tgaggcagac tttacaagaa gatttacttc gtcgattccc agatcttaac cgacttgcca
     4141 agaagtttca aagacaagca gcaaacttac aagattgtta ccgactctat cagggtataa
     4201 atcaactacc taatgttata caggctctgg aaaaacatga aggaaaacac cagaaattat
     4261 tgttggcagt ttttgtgact cctcttactg atcttcgttc tgacttctcc aagtttcagg
     4321 aaatgataga aacaacttta gatatggatc aggtggaaaa ccatgaattc cttgtaaaac
     4381 cttcatttga tcctaatctc agtgaattaa gagaaataat gaatgacttg gaaaagaaga
     4441 tgcagtcaac attaataagt gcagccagag atcttggctt ggaccctggc aaacagatta
     4501 aactggattc cagtgcacag tttggatatt actttcgtgt aacctgtaag gaagaaaaag
     4561 tccttcgtaa caataaaaac tttagtactg tagatatcca gaagaatggt gttaaattta
     4621 ccaacagcaa attgacttct ttaaatgaag agtataccaa aaataaaaca gaatatgaag
     4681 aagcccagga tgccattgtt aaagaaattg tcaatatttc ttcaggctat gtagaaccaa
     4741 tgcagacact caatgatgtg ttagctcagc tagatgctgt tgtcagcttt gctcacgtgt
     4801 caaatggagc acctgttcca tatgtacgac cagccatttt ggagaaagga caaggaagaa
     4861 ttatattaaa agcatccagg catgcttgtg ttgaagttca agatgaaatt gcatttattc
     4921 ctaatgacgt atactttgaa aaagataaac agatgttcca catcattact ggccccaata
     4981 tgggaggtaa atcaacatat attcgacaaa ctggggtgat agtactcatg gcccaaattg
     5041 ggtgttttgt gccatgtgag tcagcagaag tgtccattgt ggactgcatc ttagcccgag
     5101 taggggctgg tgacagtcaa ttgaaaggag tctccacgtt catggctgaa atgttggaaa
     5161 ctgcttctat cctcaggtct gcaaccaaag attcattaat aatcatagat gaattgggaa
     5221 gaggaacttc tacctacgat ggatttgggt tagcatgggc tatatcagaa tacattgcaa
     5281 caaagattgg tgctttttgc atgtttgcaa cccattttca tgaacttact gccttggcca
     5341 atcagatacc aactgttaat aatctacatg tcacagcact caccactgaa gagaccttaa
     5401 ctatgcttta tcaggtgaag aaaggtgtct gtgatcaaag ttttgggatt catgttgcag
     5461 agcttgctaa tttccctaag catgtaatag agtgtgctaa acagaaagcc ctggaacttg
     5521 aggagtttca gtatattgga gaatcgcaag gatatgatat catggaacca gcagcaaaga
     5581 agtgctatct ggaaagagag caaggtgaaa aaattattca ggagttcctg tccaaggtga
     5641 aacaaatgcc ctttactgaa atgtcagaag aaaacatcac aataaagtta aaacagctaa
     5701 aagctgaagt aatagcaaag aataatagct ttgtaaatga aatcatttca cgaataaaag
     5761 ttactacgtg aaaaatccca gtaatggaat gaaggtaata ttgataagct attgtctgta
     5821 atagttttat attgttttat attaaccctt tttccatagt gttaactgtc agtgcccatg
     5881 ggctatcaac ttaataagat atttagtaat attttacttt gaggacattt tcaaagattt
     5941 ttattttgaa aaatgagagc tgtaactgag gactgtttgc aattgacata ggcaataata
     6001 agtgatgtgc tgaattttat aaataaaatc atgtagtttg tggaaaaaaa aaaaaaaaaa
     6061 ngg
//
