LOCUS       sequence-250410-        5855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vimentin labeling with near-infrared fluorescent protein 
            miRFP670nano.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Oliinyk OS, Shemetov AA, Pletnev S, Shcherbakova DM, Verkhusha VV
  TITLE     Smallest near-infrared fluorescent protein evolved from 
            cyanobacteriochrome as versatile tag for spectral multiplexing.
  JOURNAL   Nat Commun. 2019 Jan 17;10(1):279. doi: 10.1038/s41467-018-08050-8.
  PUBMED    30655515
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-17"; volume: "10(1):279. doi"; pages: " 
            10.1038/s41467-018-08050-8"
COMMENT     Sequence Label: pVimentin-miRFP670nano
FEATURES             Location/Qualifiers
     source          1..5855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        92..395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        396..599
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     550..570
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2674..2795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2711..2730)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2765..2784
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2802..3257)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2939..2960)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3151..3170
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3284..3388
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3390..3747
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3390..3581
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3413..3433)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3598..3733
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3782..4576
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3782..4576
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3836..3855)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4446..4465
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4764..4783)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4808..4855
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5184..5772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5673..5692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt
       61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      241 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      361 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      421 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      481 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      541 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
      601 gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg
      661 aattcccatg tccaccaggt ccgtgtcctc gtcctcctac cgcaggatgt tcggcggccc
      721 gggcaccgcg agccggccga gctccagccg gagctacgtg actacgtcca cccgcaccta
      781 cagcctgggc agcgcgctgc gccccagcac cagccgcagc ctctacgcct cgtccccggg
      841 cggcgtgtat gccacgcgct cctctgccgt gcgcctgcgg agcagcgtgc ccggggtgcg
      901 gctcctgcag gactcggtgg acttctcgct ggccgacgcc atcaacaccg agttcaagaa
      961 cacccgcacc aacgagaagg tggagctgca ggagctgaat gaccgcttcg ccaactacat
     1021 cgacaaggtg cgcttcctgg agcagcagaa taagatcctg ctggccgagc tcgagcagct
     1081 caagggccaa ggcaagtcgc gcctggggga cctctacgag gaggagatgc gggagctgcg
     1141 ccggcaggtg gaccagctaa ccaacgacaa agcccgcgtc gaggtggagc gcgacaacct
     1201 ggccgaggac atcatgcgcc tccgggagaa attgcaggag gagatgcttc agagagagga
     1261 agccgaaaac accctgcaat ctttcagaca ggatgttgac aatgcgtctc tggcacgtct
     1321 tgaccttgaa cgcaaagtgg aatctttgca agaagagatt gcctttttga agaaactcca
     1381 cgaagaggaa atccaggagc tgcaggctca gattcaggaa cagcatgtcc aaatcgatgt
     1441 ggatgtttcc aagcctgacc tcacggctgc cctgcgtgac gtacgtcagc aatatgaaag
     1501 tgtggctgcc aagaacctgc aggaggcaga agaatggtac aaatccaagt ttgctgacct
     1561 ctctgaggct gccaaccgga acaatgacgc cctgcgccag gcaaagcagg agtccactga
     1621 gtaccggaga caggtgcagt ccctcacctg tgaagtggat gcccttaaag gaaccaatga
     1681 gtccctggaa cgccagatgc gtgaaatgga agagaacttt gccgttgaag ctgctaacta
     1741 ccaagacact attggccgcc tgcaggatga gattcagaat atgaaggagg aaatggctcg
     1801 tcaccttcgt gaataccaag acctgctcaa tgttaagatg gcccttgaca ttgagattgc
     1861 cacctacagg aagctgctgg aaggcgagga gagcaggatt tctctgcctc ttccaaactt
     1921 ttcctccctg aacctgaggg aaactaatct ggattcactc cctctggttg atacccactc
     1981 aaaaaggaca cttctgatta agacggttga aactagagat ggacaggtta tcaacgaaac
     2041 ttctcagcat cacgatgacc ttgaagggga tccaccggta atggcaaacc tggacaagat
     2101 gctgaatacc acagtaacag aggtgcggca gttcctgcag gtggacagag tgtgcgtgtt
     2161 ccagtttgag gaggattata gcggagtggt ggtggtggag gccgtggacg ataggtggat
     2221 ctccatcctg aagacccagg tgcgggatag atacttcatg gagacaaggg gcgaggagta
     2281 ttctcacggc cgctaccagg ccatcgccga catctacacc gcaaacctga cagagtgcta
     2341 cagggatctg ctgacacagt ttcaggtgag agcaatcctg gccgtgccca tcctgcaggg
     2401 caagaagctg tggggcctgt tggtggcaca ccagctggcg gcccctagac agtggcagac
     2461 ctgggagatc gactttctga agcagcaggc cgtggtggtg ggcatcgcca tccagcagag
     2521 ctagctgaat tctgcagata tccagcacag tggcggccgc gactctagat cataatcagc
     2581 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2641 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     2701 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     2761 agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat
     2821 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     2881 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     2941 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     3001 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3061 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     3121 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     3181 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     3241 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3301 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3361 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     3421 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3481 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     3541 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     3601 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3661 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     3721 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     3781 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     3841 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     3901 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     3961 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4021 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4081 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4141 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4201 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4261 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     4321 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4381 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     4441 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4501 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     4561 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     4621 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     4681 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     4741 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     4801 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     4861 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     4921 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     4981 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     5041 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5101 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5161 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5221 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5281 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5341 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5401 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5461 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5521 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5581 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5641 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5701 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5761 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5821 tttgctcaca tgttctttcc tgcgttatcc cctga
//
