LOCUS       sequence-250445-       10790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF W531C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-W531C
FEATURES             Location/Qualifiers
     source          1..10790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3..47
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    340..457
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        505..1683
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          736..1674
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1631..1651
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1703..1727
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1728..1737
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4032..4056)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4064..4082
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4107..4126)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4108..4126)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4132..4152)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             4206..4247
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4268..4835
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4285..4837
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4429..4446)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4656..4675
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4849..5448
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4849..4868)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5345..5365
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5462..6050
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5515..5535)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5933..5944)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6125..6358
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6477..6495)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6563..6667
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6668..6736,6737..7528)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6886..6905)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7699..8287
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8188..8207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8441..8458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8698..8719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8734..8739,8740..8757,8758..8764)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8772..8788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8777..8799
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8796..8812
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8796..8812
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8862..8881
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9073..9706
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9753..9878
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10375..10608
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
       61 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
      121 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
      181 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
      241 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
      301 ggtatcgccg aattcacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
      361 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
      421 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
      481 agatccagtt tggactagtc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg
      541 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
      601 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
      661 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
      721 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
      781 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
      841 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
      901 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
      961 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     1021 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
     1081 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     1141 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     1201 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     1261 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     1321 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     1381 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     1441 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
     1501 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     1561 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     1621 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     1681 tgaagcggcc ctgcagatat caacaagttt gtacaaaaaa gcaggcacca tggcggcgct
     1741 gagcggtggc ggtggtggcg gcgcggagcc gggccaggct ctgttcaacg gggacatgga
     1801 gcccgaggcc ggcgccggcg ccggcgccgc ggcctcttcg gctgcggacc ctgccattcc
     1861 ggaggaggtg tggaatatca aacaaatgat taagttgaca caggaacata tagaggccct
     1921 attggacaaa tttggtgggg agcataatcc accatcaata tatctggagg cctatgaaga
     1981 atacaccagc aagctagatg cactccaaca aagagaacaa cagttattgg aatctctggg
     2041 gaacggaact gatttttctg tttctagctc tgcatcaatg gataccgtta catcttcttc
     2101 ctcttctagc ctttcagtgc taccttcatc tctttcagtt tttcaaaatc ccacagatgt
     2161 ggcacggagc aaccccaagt caccacaaaa acctatcgtt agagtcttcc tgcccaacaa
     2221 acagaggaca gtggtacctg caaggtgtgg agttacagtc cgagacagtc taaagaaagc
     2281 actgatgatg agaggtctaa tcccagagtg ctgtgctgtt tacagaattc aggatggaga
     2341 gaagaaacca attggttggg acactgatat ttcctggctt actggagaag aattgcatgt
     2401 ggaagtgttg gagaatgttc cacttacaac acacaacttt gtacgaaaaa cgtttttcac
     2461 cttagcattt tgtgactttt gtcgaaagct gcttttccag ggtttccgct gtcaaacatg
     2521 tggttataaa tttcaccagc gttgtagtac agaagttcca ctgatgtgtg ttaattatga
     2581 ccaacttgat ttgctgtttg tctccaagtt ctttgaacac cacccaatac cacaggaaga
     2641 ggcgtcctta gcagagactg ccctaacatc tggatcatcc ccttccgcac ccgcctcgga
     2701 ctctattggg ccccaaattc tcaccagtcc gtctccttca aaatccattc caattccaca
     2761 gcccttccga ccagcagatg aagatcatcg aaatcaattt gggcaacgag accgatcctc
     2821 atcagctccc aatgtgcata taaacacaat agaacctgtc aatattgatg acttgattag
     2881 agaccaagga tttcgtggtg atggaggatc aaccacaggt ttgtctgcta ccccccctgc
     2941 ctcattacct ggctcactaa ctaacgtgaa agccttacag aaatctccag gacctcagcg
     3001 agaaaggaag tcatcttcat cctcagaaga caggaatcga atgaaaacac ttggtagacg
     3061 ggactcgagt gatgattggg agattcctga tgggcagatt acagtgggac aaagaattgg
     3121 atctggatca tttggaacag tctacaaggg aaagtggcat ggtgatgtgg cagtgaaaat
     3181 gttgaatgtg acagcaccta cacctcagca gttacaagcc ttcaaaaatg aagtaggagt
     3241 actcaggaaa acacgacatg tgaatatcct actcttcatg ggctattcca caaagccaca
     3301 actggctatt gttacccagt gttgtgaggg ctccagcttg tatcaccatc tccatatcat
     3361 tgagaccaaa tttgagatga tcaaacttat agatattgca cgacagactg cacagggcat
     3421 ggattactta cacgccaagt caatcatcca cagagacctc aagagtaata atatatttct
     3481 tcatgaagac ctcacagtaa aaataggtga ttttggtcta gctacagtga aatctcgatg
     3541 gagtgggtcc catcagtttg aacagttgtc tggatccatt ttgtggatgg caccagaagt
     3601 catcagaatg caagataaaa atccatacag ctttcagtca gatgtatatg catttggaat
     3661 tgttctgtat gaattgatga ctggacagtt accttattca aacatcaaca acagggacca
     3721 gataattttt atggtgggac gaggatacct gtctccagat ctcagtaagg tacggagtaa
     3781 ctgtccaaaa gccatgaaga gattaatggc agagtgcctc aaaaagaaaa gagatgagag
     3841 accactcttt ccccaaattc tcgcctctat tgagctgctg gcccgctcat tgccaaaaat
     3901 tcaccgcagt gcatcagaac cctccttgaa tcgggctggt ttccaaacag aggattttag
     3961 tctatatgct tgtgcttctc caaaaacacc catccaggca gggggatatg gtgcgtttcc
     4021 tgtccactag aacccagctt tcttgtacaa agtggtgata tccaattaac cctcactaaa
     4081 ggtttgttag aatgagaaga tttatgccct atagtgagtc gtattaccac ccaacttttc
     4141 tatacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt
     4201 tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt
     4261 aatgagatcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     4321 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     4381 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     4441 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     4501 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     4561 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     4621 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     4681 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     4741 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     4801 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacacatat gaccgagtac
     4861 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     4921 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     4981 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     5041 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     5101 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     5161 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     5221 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     5281 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     5341 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     5401 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctaaat cgatagatcc
     5461 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5521 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5581 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     5641 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     5701 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     5761 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     5821 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     5881 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     5941 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6001 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct
     6061 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6121 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     6181 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6241 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6301 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6361 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     6421 agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct
     6481 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     6541 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6601 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6661 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6721 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6781 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6841 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6901 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6961 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7021 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7081 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7141 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7201 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7261 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7321 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7381 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7441 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7501 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7561 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7621 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7681 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7741 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7801 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7861 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7921 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7981 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8041 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8101 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8161 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8221 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8281 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8341 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8401 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8461 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     8521 cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     8581 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     8641 ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     8701 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     8761 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac
     8821 gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8881 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
     8941 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
     9001 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
     9061 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
     9121 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     9181 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     9241 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     9301 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     9361 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     9421 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     9481 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     9541 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9601 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9661 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     9721 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     9781 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     9841 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     9901 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     9961 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
    10021 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
    10081 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
    10141 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
    10201 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
    10261 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
    10321 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
    10381 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
    10441 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
    10501 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
    10561 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
    10621 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
    10681 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    10741 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
//
