LOCUS       sequence-250450-       10790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF Y656D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-Y656D
FEATURES             Location/Qualifiers
     source          1..10790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    294..411
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        459..1637
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          690..1628
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1585..1605
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1657..1681
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1682..1691
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3986..4010)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4018..4036
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4061..4080)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4062..4080)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4086..4106)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             4160..4201
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4222..4789
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4239..4791
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4383..4400)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4610..4629
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4803..5402
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4803..4822)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5299..5319
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5416..6004
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5469..5489)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5887..5898)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6079..6312
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6431..6449)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6517..6621
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6622..6690,6691..7482)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6840..6859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7653..8241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8142..8161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8395..8412
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8652..8673
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8688..8693,8694..8711,8712..8718)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8726..8742
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8731..8753
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8750..8766
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8750..8766
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8816..8835
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9027..9660
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9707..9832
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10329..10562
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             join(10747..10790,1)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
       61 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
      121 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
      181 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
      241 gaacggatct cgacggtatc gccgaattca caaatggcag tattcatcca caattttaaa
      301 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
      361 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
      421 tacagggaca gcagagatcc agtttggact agtcgtgagg ctccggtgcc cgtcagtggg
      481 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
      541 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
      601 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
      661 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
      721 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
      781 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
      841 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
      901 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
      961 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     1021 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     1081 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     1141 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     1201 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     1261 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     1321 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     1381 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     1441 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     1501 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     1561 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     1621 catttcaggt gtcgtgaagc ggccctgcag atatcaacaa gtttgtacaa aaaagcaggc
     1681 accatggcgg cgctgagcgg tggcggtggt ggcggcgcgg agccgggcca ggctctgttc
     1741 aacggggaca tggagcccga ggccggcgcc ggcgccggcg ccgcggcctc ttcggctgcg
     1801 gaccctgcca ttccggagga ggtgtggaat atcaaacaaa tgattaagtt gacacaggaa
     1861 catatagagg ccctattgga caaatttggt ggggagcata atccaccatc aatatatctg
     1921 gaggcctatg aagaatacac cagcaagcta gatgcactcc aacaaagaga acaacagtta
     1981 ttggaatctc tggggaacgg aactgatttt tctgtttcta gctctgcatc aatggatacc
     2041 gttacatctt cttcctcttc tagcctttca gtgctacctt catctctttc agtttttcaa
     2101 aatcccacag atgtggcacg gagcaacccc aagtcaccac aaaaacctat cgttagagtc
     2161 ttcctgccca acaaacagag gacagtggta cctgcaaggt gtggagttac agtccgagac
     2221 agtctaaaga aagcactgat gatgagaggt ctaatcccag agtgctgtgc tgtttacaga
     2281 attcaggatg gagagaagaa accaattggt tgggacactg atatttcctg gcttactgga
     2341 gaagaattgc atgtggaagt gttggagaat gttccactta caacacacaa ctttgtacga
     2401 aaaacgtttt tcaccttagc attttgtgac ttttgtcgaa agctgctttt ccagggtttc
     2461 cgctgtcaaa catgtggtta taaatttcac cagcgttgta gtacagaagt tccactgatg
     2521 tgtgttaatt atgaccaact tgatttgctg tttgtctcca agttctttga acaccaccca
     2581 ataccacagg aagaggcgtc cttagcagag actgccctaa catctggatc atccccttcc
     2641 gcacccgcct cggactctat tgggccccaa attctcacca gtccgtctcc ttcaaaatcc
     2701 attccaattc cacagccctt ccgaccagca gatgaagatc atcgaaatca atttgggcaa
     2761 cgagaccgat cctcatcagc tcccaatgtg catataaaca caatagaacc tgtcaatatt
     2821 gatgacttga ttagagacca aggatttcgt ggtgatggag gatcaaccac aggtttgtct
     2881 gctacccccc ctgcctcatt acctggctca ctaactaacg tgaaagcctt acagaaatct
     2941 ccaggacctc agcgagaaag gaagtcatct tcatcctcag aagacaggaa tcgaatgaaa
     3001 acacttggta gacgggactc gagtgatgat tgggagattc ctgatgggca gattacagtg
     3061 ggacaaagaa ttggatctgg atcatttgga acagtctaca agggaaagtg gcatggtgat
     3121 gtggcagtga aaatgttgaa tgtgacagca cctacacctc agcagttaca agccttcaaa
     3181 aatgaagtag gagtactcag gaaaacacga catgtgaata tcctactctt catgggctat
     3241 tccacaaagc cacaactggc tattgttacc cagtggtgtg agggctccag cttgtatcac
     3301 catctccata tcattgagac caaatttgag atgatcaaac ttatagatat tgcacgacag
     3361 actgcacagg gcatggatta cttacacgcc aagtcaatca tccacagaga cctcaagagt
     3421 aataatatat ttcttcatga agacctcaca gtaaaaatag gtgattttgg tctagctaca
     3481 gtgaaatctc gatggagtgg gtcccatcag tttgaacagt tgtctggatc cattttgtgg
     3541 atggcaccag aagtcatcag aatgcaagat aaaaatccat acagctttca gtcagatgta
     3601 tatgcatttg gaattgttct gtatgaattg atgactggac agttacctga ttcaaacatc
     3661 aacaacaggg accagataat ttttatggtg ggacgaggat acctgtctcc agatctcagt
     3721 aaggtacgga gtaactgtcc aaaagccatg aagagattaa tggcagagtg cctcaaaaag
     3781 aaaagagatg agagaccact ctttccccaa attctcgcct ctattgagct gctggcccgc
     3841 tcattgccaa aaattcaccg cagtgcatca gaaccctcct tgaatcgggc tggtttccaa
     3901 acagaggatt ttagtctata tgcttgtgct tctccaaaaa cacccatcca ggcaggggga
     3961 tatggtgcgt ttcctgtcca ctagaaccca gctttcttgt acaaagtggt gatatccaat
     4021 taaccctcac taaaggagag aaattgaatt tagaaatgtg ccctatagtg agtcgtatta
     4081 ccacccaact tttctataca aagtggttga tatccagcac agtggcggcc gctcgagtct
     4141 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     4201 gcgtaccggt tagtaatgag atccctcccc cccccctaac gttactggcc gaagccgctt
     4261 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     4321 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     4381 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     4441 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     4501 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     4561 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     4621 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     4681 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     4741 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac
     4801 atatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     4861 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     4921 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     4981 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5041 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5101 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     5161 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     5221 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     5281 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     5341 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     5401 aaatcgatag atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5461 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5521 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5581 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5641 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5701 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5761 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     5821 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5881 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5941 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6001 ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6061 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     6121 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6181 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6241 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6301 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6361 aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga
     6421 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     6481 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6541 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6601 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6661 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6721 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6781 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6841 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     6901 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6961 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7021 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7081 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7141 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     7201 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7261 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7321 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7381 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7441 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7501 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7561 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7621 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7681 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7741 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7801 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7861 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7921 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7981 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8041 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8101 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8161 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8221 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8281 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8341 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8401 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8461 ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg
     8521 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8581 gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag
     8641 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     8701 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     8761 tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8821 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     8881 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     8941 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     9001 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     9061 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     9121 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     9181 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     9241 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     9301 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     9361 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     9421 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     9481 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     9541 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9601 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9661 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     9721 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     9781 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     9841 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     9901 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     9961 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
    10021 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
    10081 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
    10141 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
    10201 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    10261 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    10321 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    10381 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    10441 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    10501 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    10561 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    10621 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    10681 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    10741 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
//
