LOCUS       sequence-250451-        9952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of AKT3 M339I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 9952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-AKT3-M339I
FEATURES             Location/Qualifiers
     source          1..9952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        166..1344
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          397..1335
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1292..1312
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1364..1388
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2832..2856)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2909..2950
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2971..3538
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2988..3540
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3132..3149)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3359..3378
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3552..3554,3555..3557,3558..4271)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3552..3554,3555..3557,3558..4268)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3597..3618)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3858..3877)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4205..4226
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4285..4873
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4338..4358)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4756..4767)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4948..5181
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5300..5318)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5386..5490
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5491..5559,5560..6351)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5709..5728)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6522..7110
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7011..7030
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7264..7281
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7521..7542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7557..7562,7563..7580,7581..7587)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7595..7611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7600..7622
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7619..7635
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7619..7635
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7685..7704
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7896..8529
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8576..8701
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9198..9431
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9616..9660
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccagt ttggactagt cgtgaggctc cggtgcccgt
      181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
      241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
      421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
      481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
      541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
      601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
      661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
      721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     1021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     1081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     1141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     1201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     1261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     1321 tttcttccat ttcaggtgtc gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa
     1381 agcaggcacc atgagcgatg ttaccattgt gaaagaaggt tgggttcaga agaggggaga
     1441 atatataaaa aactggaggc caagatactt ccttttgaag acagatggct cattcatagg
     1501 atataaagag aaacctcaag atgtggattt accttatccc ctcaacaact tttcagtggc
     1561 aaaatgccag ttaatgaaaa cagaacgacc aaagccaaac acatttataa tcagatgtct
     1621 ccagtggact actgttatag agagaacatt tcatgtagat actccagagg aaagggaaga
     1681 atggacagaa gctatccagg ctgtagcaga cagactgcag aggcaagaag aggagagaat
     1741 gaattgtagt ccaacttcac aaattgataa tataggagag gaagagatgg atgcctctac
     1801 aacccatcat aaaagaaaga caatgaatga ttttgactat ttgaaactac taggtaaagg
     1861 cacttttggg aaagttattt tggttcgaga gaaggcaagt ggaaaatact atgctatgaa
     1921 gattctgaag aaagaagtca ttattgcaaa ggatgaagtg gcacacactc taactgaaag
     1981 cagagtatta aagaacacta gacatccctt tttaacatcc ttgaaatatt ccttccagac
     2041 aaaagaccgt ttgtgttttg tgatggaata tgttaatggg ggcgagctgt ttttccattt
     2101 gtcgagagag cgggtgttct ctgaggaccg cacacgtttc tatggtgcag aaattgtctc
     2161 tgccttggac tatctacatt ccggaaagat tgtgtaccgt gatctcaagt tggagaatct
     2221 aatgctggac aaagatggcc acataaaaat tacagatttt ggactttgca aagaagggat
     2281 cacagatgca gccaccatga agacattctg tggcactcca gaatatctgg caccagaggt
     2341 gttagaagat aatgactatg gccgagcagt agactggtgg ggcctagggg ttgtcatgta
     2401 tgaaatcatg tgtgggaggt tacctttcta caaccaggac catgagaaac tttttgaatt
     2461 aatattaatg gaagacatta aatttcctcg aacactctct tcagatgcaa aatcattgct
     2521 ttcagggctc ttgataaagg atccaaataa acgccttggt ggaggaccag atgatgcaaa
     2581 agaaattatg agacacagtt tcttctctgg agtaaactgg caagatgtat atgataaaaa
     2641 gcttgtacct ccttttaaac ctcaagtaac atctgagaca gatactagat attttgatga
     2701 agaatttaca gctcagacta ttacaataac accacctgaa aaatatgatg aggatggtat
     2761 ggactgcatg gacaatgaga ggcggccgca tttccctcaa ttttcctact ctgcaagtgg
     2821 acgagaatag aacccagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg
     2881 ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     2941 cgattctacg cgtaccggtt agtaatgaga tccctccccc ccccctaacg ttactggccg
     3001 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     3061 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     3121 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     3181 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     3241 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     3301 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     3361 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     3421 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     3481 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     3541 tggccacaca tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3601 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3661 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3721 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3781 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3841 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     3901 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3961 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     4021 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     4081 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4141 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     4201 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4261 tgtacaagta aatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg
     4321 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4381 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4441 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4501 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4561 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4621 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     4681 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4741 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4801 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4861 gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta
     4921 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     4981 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5041 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5101 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5161 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     5221 ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat
     5281 cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5341 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5401 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5461 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5521 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5581 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5641 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5701 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     5761 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5821 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5881 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5941 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6001 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6061 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6121 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6181 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6241 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6301 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6361 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6421 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6481 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6541 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6601 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6661 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6721 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6781 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6841 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6901 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6961 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7021 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7081 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7141 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7201 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7261 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7321 gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg
     7381 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7441 gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg
     7501 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7561 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7621 ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc actgcattct
     7681 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     7741 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     7801 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     7861 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
     7921 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     7981 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     8041 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     8101 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     8161 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     8221 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     8281 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     8341 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8401 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8461 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8521 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     8581 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     8641 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     8701 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     8761 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     8821 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     8881 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     8941 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     9001 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
     9061 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     9121 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     9181 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     9241 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     9301 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     9361 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     9421 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     9481 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     9541 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     9601 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     9661 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     9721 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     9781 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     9841 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     9901 aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac aa
//
