LOCUS       sequence-250455-       13375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ALK C990R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13375)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 13375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ALK-C990R
FEATURES             Location/Qualifiers
     source          1..13375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        166..1344
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          397..1335
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1292..1312
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1364..1388
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6255..6279)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6332..6373
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6394..6961
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6411..6963
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6555..6572)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6782..6801
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6975..6977,6978..6980,6981..7694)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6975..6977,6978..6980,6981..7691)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7020..7041)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7281..7300)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7628..7649
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7708..8296
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7761..7781)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8179..8190)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8371..8604
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8723..8741)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8809..8913
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8914..8982,8983..9774)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9132..9151)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9945..10533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10434..10453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10687..10704
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10944..10965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10980..10985,10986..11003,11004..11010)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11018..11034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11023..11045
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11042..11058
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11042..11058
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11108..11127
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11319..11952
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11999..12124
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12621..12854
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13039..13083
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccagt ttggactagt cgtgaggctc cggtgcccgt
      181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
      241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
      421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
      481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
      541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
      601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
      661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
      721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     1021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     1081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     1141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     1201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     1261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     1321 tttcttccat ttcaggtgtc gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa
     1381 agcaggcacc atgggagcca tcgggctcct gtggctcctg ccgctgctgc tttccacggc
     1441 agctgtgggc tccgggatgg ggaccggcca gcgcgcgggc tccccagctg cggggccgcc
     1501 gctgcagccc cgggagccac tcagctactc gcgcctgcag aggaagagtc tggcagttga
     1561 cttcgtggtg ccctcgctct tccgtgtcta cgcccgggac ctactgctgc caccatcctc
     1621 ctcggagctg aaggctggca ggcccgaggc ccgcggctcg ctagctctgg actgcgcccc
     1681 gctgctcagg ttgctggggc cggcgccggg ggtctcctgg accgccggtt caccagcccc
     1741 ggcagaggcc cggacgctgt ccagggtgct gaagggcggc tccgtgcgca agctccggcg
     1801 tgccaagcag ttggtgctgg agctgggcga ggaggcgatc ttggagggtt gcgtcgggcc
     1861 ccccggggag gcggctgtgg ggctgctcca gttcaatctc agcgagctgt tcagttggtg
     1921 gattcgccaa ggcgaagggc gactgaggat ccgcctgatg cccgagaaga aggcgtcgga
     1981 agtgggcaga gagggaaggc tgtccgcggc aattcgcgcc tcccagcccc gccttctctt
     2041 ccagatcttc gggactggtc atagctcctt ggaatcacca acaaacatgc cttctccttc
     2101 tcctgattat tttacatgga atctcacctg gataatgaaa gactccttcc ctttcctgtc
     2161 tcatcgcagc cgatatggtc tggagtgcag ctttgacttc ccctgtgagc tggagtattc
     2221 ccctccactg catgacctca ggaaccagag ctggtcctgg cgccgcatcc cctccgagga
     2281 ggcctcccag atggacttgc tggatgggcc tggggcagag cgttctaagg agatgcccag
     2341 aggctccttt ctccttctca acacctcagc tgactccaag cacaccatcc tgagtccgtg
     2401 gatgaggagc agcagtgagc actgcacact ggccgtctcg gtgcacaggc acctgcagcc
     2461 ctctggaagg tacattgccc agctgctgcc ccacaacgag gctgcaagag agatcctcct
     2521 gatgcccact ccagggaagc atggttggac agtgctccag ggaagaatcg ggcgtccaga
     2581 caacccattt cgagtggccc tggaatacat ctccagtgga aaccgcagct tgtctgcagt
     2641 ggacttcttt gccctgaaga actgcagtga aggaacatcc ccaggctcca agatggccct
     2701 gcagagctcc ttcacttgtt ggaatgggac agtcctccag cttgggcagg cctgtgactt
     2761 ccaccaggac tgtgcccagg gagaagatga gagccagatg tgccggaaac tgcctgtggg
     2821 tttttactgc aactttgaag atggcttctg tggctggacc caaggcacac tgtcacccca
     2881 cactcctcaa tggcaggtca ggaccctaaa ggatgcccgg ttccaggacc accaagacca
     2941 tgctctattg ctcagtacca ctgatgtccc cgcttctgaa agtgctacag tgaccagtgc
     3001 tacgtttcct gcaccgatca agagctctcc atgtgagctc cgaatgtcct ggctcattcg
     3061 tggagtcttg aggggaaacg tgtccttggt gctagtggag aacaaaaccg ggaaggagca
     3121 aggcaggatg gtctggcatg tcgccgccta tgaaggcttg agcctgtggc agtggatggt
     3181 gttgcctctc ctcgatgtgt ctgacaggtt ctggctgcag atggtcgcat ggtggggaca
     3241 aggatccaga gccatcgtgg cttttgacaa tatctccatc agcctggact gctacctcac
     3301 cattagcgga gaggacaaga tcctgcagaa tacagcaccc aaatcaagaa acctgtttga
     3361 gagaaaccca aacaaggagc tgaaacccgg ggaaaattca ccaagacaga cccccatctt
     3421 tgaccctaca gttcattggc tgttcaccac atgtggggcc agcgggcccc atggccccac
     3481 ccaggcacag tgcaacaacg cctaccagaa ctccaacctg agcgtggagg tggggagcga
     3541 gggccccctg aaaggcatcc agatctggaa ggtgccagcc accgacacct acagcatctc
     3601 gggctacgga gctgctggcg ggaaaggcgg gaagaacacc atgatgcggt cccacggcgt
     3661 gtctgtgctg ggcatcttca acctggagaa ggatgacatg ctgtacatcc tggttgggca
     3721 gcagggagag gacgcctgcc ccagtacaaa ccagttaatc cagaaagtct gcattggaga
     3781 gaacaatgtg atagaagaag aaatccgtgt gaacagaagc gtgcatgagt gggcaggagg
     3841 cggaggagga gggggtggag ccacctacgt atttaagatg aaggatggag tgccggtgcc
     3901 cctgatcatt gcagccggag gtggtggcag ggcctacggg gccaagacag acacgttcca
     3961 cccagagaga ctggagaata actcctcggt tctagggcta aacggcaatt ccggagccgc
     4021 aggtggtgga ggtggctgga atgataacac ttccttgctc tgggccggaa aatctttgca
     4081 ggagggtgcc accggaggac attcctgccc ccaggccatg aagaagtggg ggtgggagac
     4141 aagagggggt ttcggagggg gtggaggggg gtgctcctca ggtggaggag gcggaggata
     4201 tataggcggc aatgcagcct caaacaatga ccccgaaatg gatggggaag atggggtttc
     4261 cttcatcagt ccactgggca tcctgtacac cccagcttta aaagtgatgg aaggccacgg
     4321 ggaagtgaat attaagcatt atctaaactg cagtcaccgt gaggtagacg aatgtcacat
     4381 ggaccctgaa agccacaagg tcatctgctt ctgtgaccac gggacggtgc tggctgagga
     4441 tggcgtctcc tgcattgtgt cacccacccc ggagccacac ctgccactct cgctgatcct
     4501 ctctgtggtg acctctgccc tcgtggccgc cctggtcctg gctttctccg gcatcatgat
     4561 tgtgtaccgc cggaagcacc aggagctgca agccatgcag atggagctgc agagccctga
     4621 gtacaagctg agcaagctcc gcacctcgac catcatgacc gactacaacc ccaactactg
     4681 ctttgctggc aagacctcct ccatcagtga cctgaaggag gtgccgcgga aaaacatcac
     4741 cctcattcgg ggtctgggcc atggcgcctt tggggaggtg tatgaaggcc aggtgtccgg
     4801 aatgcccaac gacccaagcc ccctgcaagt ggctgtgaag acgctgcctg aagtgtgctc
     4861 tgaacaggac gaactggatt tcctcatgga agccctgatc atcagcaaat tcaaccacca
     4921 gaacattgtt cgctgcattg gggtgagcct gcaatccctg ccccggttca tcctgctgga
     4981 gctcatggcg gggggagacc tcaagtcctt cctccgagag acccgccctc gcccgagcca
     5041 gccctcctcc ctggccatgc tggaccttct gcacgtggct cgggacattg cctgtggctg
     5101 tcagtatttg gaggaaaacc acttcatcca ccgagacatt gctgccagaa actgcctctt
     5161 gacctgtcca ggccctggaa gagtggccaa gattggagac ttcgggatgg cccgagacat
     5221 ctacagggcg agctactata gaaagggagg ctgtgccatg ctgccagtta agtggatgcc
     5281 cccagaggcc ttcatggaag gaatattcac ttctaaaaca gacacatggt cctttggagt
     5341 gctgctatgg gaaatctttt ctcttggata tatgccatac cccagcaaaa gcaaccagga
     5401 agttctggag tttgtcacca gtggaggccg gatggaccca cccaagaact gccctgggcc
     5461 tgtataccgg ataatgactc agtgctggca acatcagcct gaagacaggc ccaactttgc
     5521 catcattttg gagaggattg aatactgcac ccaggacccg gatgtaatca acaccgcttt
     5581 gccgatagaa tatggtccac ttgtggaaga ggaagagaaa gtgcctgtga ggcccaagga
     5641 ccctgagggg gttcctcctc tcctggtctc tcaacaggca aaacgggagg aggagcgcag
     5701 cccagctgcc ccaccacctc tgcctaccac ctcctctggc aaggctgcaa agaaacccac
     5761 agctgcagag atctctgttc gagtccctag agggccggcc gtggaagggg gacacgtgaa
     5821 tatggcattc tctcagtcca accctccttc ggagttgcac aaggtccacg gatccagaaa
     5881 caagcccacc agcttgtgga acccaacgta cggctcctgg tttacagaga aacccaccaa
     5941 aaagaataat cctatagcaa agaaggagcc acacgacagg ggtaacctgg ggctggaggg
     6001 aagctgtact gtcccaccta acgttgcaac tgggagactt ccgggggcct cactgctcct
     6061 agagccctct tcgctgactg ccaatatgaa ggaggtacct ctgttcaggc tacgtcactt
     6121 cccttgtggg aatgtcaatt acggctacca gcaacagggc ttgcccttag aagccgctac
     6181 tgcccctgga gctggtcatt acgaggatac cattctgaaa agcaagaata gcatgaacca
     6241 gcctgggccc tagaacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg
     6301 ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg
     6361 tctcgattct acgcgtaccg gttagtaatg agatccctcc ccccccccta acgttactgg
     6421 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     6481 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     6541 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     6601 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     6661 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     6721 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     6781 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     6841 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     6901 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     6961 atatggccac acatatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     7021 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     7081 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     7141 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     7201 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     7261 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     7321 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     7381 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     7441 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     7501 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     7561 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     7621 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     7681 agctgtacaa gtaaatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga
     7741 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7801 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7861 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7921 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7981 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8041 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8101 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8161 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8221 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8281 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc
     8341 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     8401 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8461 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8521 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8581 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     8641 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta
     8701 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     8761 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     8821 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8881 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8941 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9001 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9061 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9121 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     9181 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9241 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9301 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9361 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9421 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9481 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9541 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9601 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9661 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9721 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9781 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9841 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9901 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9961 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10021 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10081 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10141 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10201 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10261 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10321 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10381 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10441 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10501 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10561 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10621 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10681 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10741 cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt
    10801 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    10861 ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa
    10921 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    10981 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    11041 acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat
    11101 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    11161 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    11221 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    11281 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
    11341 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
    11401 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
    11461 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
    11521 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
    11581 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
    11641 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
    11701 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
    11761 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
    11821 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
    11881 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
    11941 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
    12001 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
    12061 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
    12121 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
    12181 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
    12241 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
    12301 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
    12361 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
    12421 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
    12481 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
    12541 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
    12601 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
    12661 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
    12721 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
    12781 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
    12841 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
    12901 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
    12961 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    13021 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    13081 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    13141 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    13201 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
    13261 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
    13321 gtgaacggat ctcgacggta tcgccgaatt cacaaatggc agtattcatc cacaa
//
