LOCUS       sequence-250457-       10598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of DDX3X E285K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10598)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-DDX3X-E285K
FEATURES             Location/Qualifiers
     source          1..10598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    200..317
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        365..1543
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          596..1534
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1491..1511
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1563..1587
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3580..3604)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3612..3630
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3655..3674)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3656..3674)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3680..3700)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3754..3795
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3816..4383
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3833..4385
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3977..3994)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4204..4223
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4397..4399,4400..4402,4403..5116)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4397..4399,4400..4402,4403..5113)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4442..4463)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4703..4722)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5050..5071
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5130..5718
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5183..5203)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5601..5612)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5793..6026
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6145..6163)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6231..6335
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6336..6404,6405..7196)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6554..6573)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7367..7955
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7856..7875
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8109..8126
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8366..8387
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8402..8407,8408..8425,8426..8432)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8440..8456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8445..8467
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8464..8480
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8464..8480
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8530..8549
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8741..9374
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9421..9546
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10043..10276
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10461..10505
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
       61 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
      121 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgccg aattcacaaa
      181 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
      241 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
      301 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggactagtc
      361 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
      421 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
      481 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
      541 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
      601 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
      661 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
      721 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
      781 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
      841 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
      901 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
      961 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     1021 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     1081 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     1141 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     1201 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     1261 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     1321 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     1381 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     1441 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     1501 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaagcggcc ctgcagatat
     1561 caacaagttt gtacaaaaaa gcaggcacca tgagtcatgt ggcagtggaa aatgcgctcg
     1621 ggctggacca gcagtttgct ggcctagacc tgaactcttc agataatcag agtggaggaa
     1681 gtacagccag caaagggcgc tatattcctc ctcatttaag gaaccgagaa gctactaaag
     1741 gtttctacga taaagacagt tcagggtgga gttctagcaa agataaggat gcgtatagca
     1801 gttttggatc tcgtagtgat tcaagaggga agtctagctt cttcagtgat cgtggaagtg
     1861 gatcaagggg aaggtttgat gatcgtggac ggagtgatta cgatggcatt ggcagccgtg
     1921 gtgacagaag tggctttggc aaatttgaac gtggtggaaa cagtcgctgg tgtgacaaat
     1981 cagatgaaga tgattggtca aaaccactcc caccaagtga acgcttggaa caggaactct
     2041 tttctggagg caacactggg attaattttg agaaatacga tgacattcca gttgaggcaa
     2101 caggcaacaa ctgtcctcca catattgaaa gtttcagtga tgttgagatg ggagaaatta
     2161 tcatgggaaa cattgagctt actcgttata ctcgcccaac tccagtgcaa aagcatgcta
     2221 ttcctattat caaagagaaa agagacttga tggcttgtgc ccaaacaggg tctggaaaaa
     2281 ctgcagcatt tctgttgccc atcttgagtc agatttattc agatggtcca ggcgaggctt
     2341 tgagggccat gaaggaaaat ggaaggtatg ggcgccgcaa acaataccca atctccttgg
     2401 tattagcacc aacgagagag ttggcagtac agatctacga gaaagccaga aaattttcat
     2461 accgatctag agttcgtcct tgcgtggttt atggtggtgc cgatattggt cagcagattc
     2521 gagacttgga acgtggatgc catttgttag tagccactcc aggacgtcta gtggatatga
     2581 tggaaagagg aaagattgga ttagactttt gcaaatactt ggtgttagat gaagctgatc
     2641 ggatgttgga tatggggttt gagcctcaga ttcgtagaat agtcgaacaa gatactatgc
     2701 ctccaaaggg tgtccgccac actatgatgt ttagtgctac ttttcctaag gaaatacaga
     2761 tgctggctcg tgatttctta gatgaatata tcttcttggc tgtaggaaga gttggctcta
     2821 cctctgaaaa catcacacag aaagtagttt gggtggaaga atcagacaaa cggtcatttc
     2881 tgcttgacct cctaaatgca acaggcaagg attcactgac cttagtgttt gtggagacca
     2941 aaaagggtgc agattctctg gaggatttct tataccatga aggatacgca tgtaccagca
     3001 tccatggaga ccgttctcag agggatagag aagaggccct tcaccagttc cgctcaggaa
     3061 aaagcccaat tttagtggct acagcagtag cagcaagagg actggacatt tcaaatgtga
     3121 aacatgttat caattttgac ttgccaagtg atattgaaga atatgtacat cgtattggtc
     3181 gtacgggacg tgtaggaaac cttggcctgg caacctcatt ctttaacgag aggaacataa
     3241 atattactaa ggatttgttg gatcttcttg ttgaagctaa acaagaagtg ccgtcttggt
     3301 tagaaaacat ggcttatgaa caccactaca agggtagcag tcgtggacgt tctaagagta
     3361 gcagatttag tggagggttt ggtgccagag actaccgaca aagtagcggt gccagcagtt
     3421 ccagcttcag cagcagccgc gcaagcagca gccgcagtgg cggaggtggc cacggtagca
     3481 gcagaggatt tggtggaggt ggctatggag gcttttacaa cagtgatgga tatggaggaa
     3541 attataactc ccagggggtt gactggtggg gtaactagaa cccagctttc ttgtacaaag
     3601 tggtgatatc caattaaccc tcactaaagg gaagatattg aaagaatttg atgtccctat
     3661 agtgagtcgt attaccaccc aacttttcta tacaaagtgg ttgatatcca gcacagtggc
     3721 ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc
     3781 ggtctcgatt ctacgcgtac cggttagtaa tgagatccct cccccccccc taacgttact
     3841 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     3901 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     3961 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     4021 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     4081 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     4141 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     4201 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     4261 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     4321 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     4381 taatatggcc acacatatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4441 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     4501 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     4561 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     4621 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     4681 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     4741 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     4801 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     4861 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     4921 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     4981 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     5041 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5101 cgagctgtac aagtaaatcg atagatccta atcaacctct ggattacaaa atttgtgaaa
     5161 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5221 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5281 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5341 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5401 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5461 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5521 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5581 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5641 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5701 tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga
     5761 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5821 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5881 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5941 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6001 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     6061 ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc
     6121 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     6181 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6241 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6301 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6361 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6421 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6481 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6541 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     6601 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6661 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6721 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6781 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6841 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6901 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6961 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7021 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7081 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7141 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7201 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7261 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7321 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7381 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7441 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7501 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7561 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7621 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7681 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7741 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7801 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7861 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7921 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7981 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8041 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8101 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8161 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat
     8221 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8281 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga
     8341 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     8401 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     8461 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc
     8521 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     8581 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     8641 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
     8701 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
     8761 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     8821 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     8881 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
     8941 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
     9001 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
     9061 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
     9121 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
     9181 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9241 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9301 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9361 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     9421 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     9481 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     9541 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     9601 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     9661 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     9721 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     9781 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     9841 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct
     9901 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     9961 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
    10021 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
    10081 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
    10141 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
    10201 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
    10261 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
    10321 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
    10381 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
    10441 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
    10501 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
    10561 tagtaggagg cttggtaggt ttaagaatag tttttgct
//
