LOCUS       sequence-250459-       13352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ALK G875R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13352)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 13352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ALK-G875R
FEATURES             Location/Qualifiers
     source          1..13352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6090..6114)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6122..6140
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6165..6184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6166..6184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6190..6210)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6264..6305
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6326..6893
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6343..6895
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6487..6504)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6714..6733
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6907..7506
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6907..6926)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7403..7423
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7520..8108
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7573..7593)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7991..8002)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8183..8416
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8535..8553)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8621..8725
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8726..8794,8795..9586)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8944..8963)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9757..10345
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10246..10265
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10499..10516
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10756..10777
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10792..10797,10798..10815,10816..10822)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10830..10846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10835..10857
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10854..10870
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10854..10870
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10920..10939
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11131..11764
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11811..11936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12433..12666
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12851..12895
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13188..13305
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccctgc agatatcaac
     1201 aagtttgtac aaaaaagcag gcaccatggg agccatcggg ctcctgtggc tcctgccgct
     1261 gctgctttcc acggcagctg tgggctccgg gatggggacc ggccagcgcg cgggctcccc
     1321 agctgcgggg ccgccgctgc agccccggga gccactcagc tactcgcgcc tgcagaggaa
     1381 gagtctggca gttgacttcg tggtgccctc gctcttccgt gtctacgccc gggacctact
     1441 gctgccacca tcctcctcgg agctgaaggc tggcaggccc gaggcccgcg gctcgctagc
     1501 tctggactgc gccccgctgc tcaggttgct ggggccggcg ccgggggtct cctggaccgc
     1561 cggttcacca gccccggcag aggcccggac gctgtccagg gtgctgaagg gcggctccgt
     1621 gcgcaagctc cggcgtgcca agcagttggt gctggagctg ggcgaggagg cgatcttgga
     1681 gggttgcgtc gggccccccg gggaggcggc tgtggggctg ctccagttca atctcagcga
     1741 gctgttcagt tggtggattc gccaaggcga agggcgactg aggatccgcc tgatgcccga
     1801 gaagaaggcg tcggaagtgg gcagagaggg aaggctgtcc gcggcaattc gcgcctccca
     1861 gccccgcctt ctcttccaga tcttcgggac tggtcatagc tccttggaat caccaacaaa
     1921 catgccttct ccttctcctg attattttac atggaatctc acctggataa tgaaagactc
     1981 cttccctttc ctgtctcatc gcagccgata tggtctggag tgcagctttg acttcccctg
     2041 tgagctggag tattcccctc cactgcatga cctcaggaac cagagctggt cctggcgccg
     2101 catcccctcc gaggaggcct cccagatgga cttgctggat gggcctgggg cagagcgttc
     2161 taaggagatg cccagaggct cctttctcct tctcaacacc tcagctgact ccaagcacac
     2221 catcctgagt ccgtggatga ggagcagcag tgagcactgc acactggccg tctcggtgca
     2281 caggcacctg cagccctctg gaaggtacat tgcccagctg ctgccccaca acgaggctgc
     2341 aagagagatc ctcctgatgc ccactccagg gaagcatggt tggacagtgc tccagggaag
     2401 aatcgggcgt ccagacaacc catttcgagt ggccctggaa tacatctcca gtggaaaccg
     2461 cagcttgtct gcagtggact tctttgccct gaagaactgc agtgaaggaa catccccagg
     2521 ctccaagatg gccctgcaga gctccttcac ttgttggaat gggacagtcc tccagcttgg
     2581 gcaggcctgt gacttccacc aggactgtgc ccagggagaa gatgagagcc agatgtgccg
     2641 gaaactgcct gtgggttttt actgcaactt tgaagatggc ttctgtggct ggacccaagg
     2701 cacactgtca ccccacactc ctcaatggca ggtcaggacc ctaaaggatg cccggttcca
     2761 ggaccaccaa gaccatgctc tattgctcag taccactgat gtccccgctt ctgaaagtgc
     2821 tacagtgacc agtgctacgt ttcctgcacc gatcaagagc tctccatgtg agctccgaat
     2881 gtcctggctc attcgtggag tcttgagggg aaacgtgtcc ttggtgctag tggagaacaa
     2941 aaccgggaag gagcaaggca ggatggtctg gcatgtcgcc gcctatgaag gcttgagcct
     3001 gtggcagtgg atggtgttgc ctctcctcga tgtgtctgac aggttctggc tgcagatggt
     3061 cgcatggtgg ggacaaggat ccagagccat cgtggctttt gacaatatct ccatcagcct
     3121 ggactgctac ctcaccatta gcggagagga caagatcctg cagaatacag cacccaaatc
     3181 aagaaacctg tttgagagaa acccaaacaa ggagctgaaa cccggggaaa attcaccaag
     3241 acagaccccc atctttgacc ctacagttca ttggctgttc accacatgtg gggccagcgg
     3301 gccccatggc cccacccagg cacagtgcaa caacgcctac cagaactcca acctgagcgt
     3361 ggaggtgggg agcgagggcc ccctgaaagg catccagatc tggaaggtgc cagccaccga
     3421 cacctacagc atctcgggct acggagctgc tggcgggaaa ggcgggaaga acaccatgat
     3481 gcggtcccac ggcgtgtctg tgctgggcat cttcaacctg gagaaggatg acatgctgta
     3541 catcctggtt gggcagcagg gagaggacgc ctgccccagt acaaaccagt taatccagaa
     3601 agtctgcatt ggagagaaca atgtgataga agaagaaatc cgtgtgaaca gaagcgtgca
     3661 tgagtgggca ggaggcggag gaggaggggg tggagccacc tacgtattta agatgaagga
     3721 tggagtgccg gtgcccctga tcattgcagc cggaggtggt ggcagggcct acggggccaa
     3781 gacagacacg ttccacccag agagactgga gaataactcc tcggttctag ggctaaacgg
     3841 caattccaga gccgcaggtg gtggaggtgg ctggaatgat aacacttcct tgctctgggc
     3901 cggaaaatct ttgcaggagg gtgccaccgg aggacattcc tgcccccagg ccatgaagaa
     3961 gtgggggtgg gagacaagag ggggtttcgg agggggtgga ggggggtgct cctcaggtgg
     4021 aggaggcgga ggatatatag gcggcaatgc agcctcaaac aatgaccccg aaatggatgg
     4081 ggaagatggg gtttccttca tcagtccact gggcatcctg tacaccccag ctttaaaagt
     4141 gatggaaggc cacggggaag tgaatattaa gcattatcta aactgcagtc actgtgaggt
     4201 agacgaatgt cacatggacc ctgaaagcca caaggtcatc tgcttctgtg accacgggac
     4261 ggtgctggct gaggatggcg tctcctgcat tgtgtcaccc accccggagc cacacctgcc
     4321 actctcgctg atcctctctg tggtgacctc tgccctcgtg gccgccctgg tcctggcttt
     4381 ctccggcatc atgattgtgt accgccggaa gcaccaggag ctgcaagcca tgcagatgga
     4441 gctgcagagc cctgagtaca agctgagcaa gctccgcacc tcgaccatca tgaccgacta
     4501 caaccccaac tactgctttg ctggcaagac ctcctccatc agtgacctga aggaggtgcc
     4561 gcggaaaaac atcaccctca ttcggggtct gggccatggc gcctttgggg aggtgtatga
     4621 aggccaggtg tccggaatgc ccaacgaccc aagccccctg caagtggctg tgaagacgct
     4681 gcctgaagtg tgctctgaac aggacgaact ggatttcctc atggaagccc tgatcatcag
     4741 caaattcaac caccagaaca ttgttcgctg cattggggtg agcctgcaat ccctgccccg
     4801 gttcatcctg ctggagctca tggcgggggg agacctcaag tccttcctcc gagagacccg
     4861 ccctcgcccg agccagccct cctccctggc catgctggac cttctgcacg tggctcggga
     4921 cattgcctgt ggctgtcagt atttggagga aaaccacttc atccaccgag acattgctgc
     4981 cagaaactgc ctcttgacct gtccaggccc tggaagagtg gccaagattg gagacttcgg
     5041 gatggcccga gacatctaca gggcgagcta ctatagaaag ggaggctgtg ccatgctgcc
     5101 agttaagtgg atgcccccag aggccttcat ggaaggaata ttcacttcta aaacagacac
     5161 atggtccttt ggagtgctgc tatgggaaat cttttctctt ggatatatgc cataccccag
     5221 caaaagcaac caggaagttc tggagtttgt caccagtgga ggccggatgg acccacccaa
     5281 gaactgccct gggcctgtat accggataat gactcagtgc tggcaacatc agcctgaaga
     5341 caggcccaac tttgccatca ttttggagag gattgaatac tgcacccagg acccggatgt
     5401 aatcaacacc gctttgccga tagaatatgg tccacttgtg gaagaggaag agaaagtgcc
     5461 tgtgaggccc aaggaccctg agggggttcc tcctctcctg gtctctcaac aggcaaaacg
     5521 ggaggaggag cgcagcccag ctgccccacc acctctgcct accacctcct ctggcaaggc
     5581 tgcaaagaaa cccacagctg cagagatctc tgttcgagtc cctagagggc cggccgtgga
     5641 agggggacac gtgaatatgg cattctctca gtccaaccct ccttcggagt tgcacaaggt
     5701 ccacggatcc agaaacaagc ccaccagctt gtggaaccca acgtacggct cctggtttac
     5761 agagaaaccc accaaaaaga ataatcctat agcaaagaag gagccacacg acaggggtaa
     5821 cctggggctg gagggaagct gtactgtccc acctaacgtt gcaactggga gacttccggg
     5881 ggcctcactg ctcctagagc cctcttcgct gactgccaat atgaaggagg tacctctgtt
     5941 caggctacgt cacttccctt gtgggaatgt caattacggc taccagcaac agggcttgcc
     6001 cttagaagcc gctactgccc ctggagctgg tcattacgag gataccattc tgaaaagcaa
     6061 gaatagcatg aaccagcctg ggccctagaa cccagctttc ttgtacaaag tggtgatatc
     6121 caattaaccc tcactaaagg tttgatttat gtgttatgtt gagtccctat agtgagtcgt
     6181 attaccaccc aacttttcta tacaaagtgg ttgatatcca gcacagtggc ggccgctcga
     6241 gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt
     6301 ctacgcgtac cggttagtaa tgagatccct cccccccccc taacgttact ggccgaagcc
     6361 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     6421 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     6481 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     6541 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     6601 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     6661 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     6721 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     6781 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     6841 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     6901 acacatatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6961 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     7021 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     7081 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     7141 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     7201 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     7261 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     7321 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     7381 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     7441 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     7501 gcctaaatcg atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7561 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7621 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7681 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7741 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7801 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7861 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     7921 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7981 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     8041 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     8101 ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     8161 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     8221 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8281 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8341 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8401 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     8461 aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc
     8521 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     8581 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     8641 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     8701 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     8761 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     8821 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     8881 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     8941 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     9001 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     9061 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     9121 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     9181 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     9241 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     9301 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     9361 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     9421 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     9481 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     9541 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     9601 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     9661 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9721 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     9781 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     9841 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     9901 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     9961 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10021 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10081 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10141 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    10201 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    10261 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    10321 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    10381 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    10441 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    10501 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
    10561 ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag
    10621 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    10681 ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag
    10741 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    10801 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    10861 cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg
    10921 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    10981 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    11041 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    11101 ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac
    11161 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
    11221 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
    11281 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
    11341 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
    11401 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
    11461 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
    11521 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
    11581 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    11641 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
    11701 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    11761 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
    11821 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
    11881 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
    11941 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
    12001 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
    12061 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
    12121 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
    12181 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
    12241 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
    12301 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
    12361 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
    12421 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
    12481 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
    12541 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
    12601 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
    12661 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
    12721 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
    12781 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
    12841 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
    12901 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
    12961 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
    13021 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
    13081 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
    13141 atctcgacgg tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa
    13201 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
    13261 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
    13321 gacagcagag atccagtttg gactagtcgt ga
//
