LOCUS       sequence-250466-       12145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR A289D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12145)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-A289D
FEATURES             Location/Qualifiers
     source          1..12145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    159..276
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        324..1502
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          555..1493
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1450..1470
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1522..1546
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1549..1620,1621..3483,3484..3552,3553..5181)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGDTCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5183..5207)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5260..5301
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5322..5889
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5339..5891
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5483..5500)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5710..5729
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5903..5905,5906..5908,5909..6622)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5903..5905,5906..5908,5909..6619)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5948..5969)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6209..6228)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6556..6577
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6636..7224
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6689..6709)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7107..7118)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7299..7532
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7651..7669)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7737..7841
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7842..7910,7911..8702)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8873..9461
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9362..9381
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9615..9632
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9872..9893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9908..9913,9914..9931,9932..9938)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9946..9962
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9951..9973
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9970..9986
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9970..9986
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10036..10055
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10247..10880
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10927..11052
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11549..11782
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11967..12011
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
       61 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
      121 gtatcgccga attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
      181 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
      241 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
      301 gatccagttt ggactagtcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc
      361 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
      421 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
      481 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
      541 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
      601 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
      661 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
      721 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
      781 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
      841 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
      901 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
      961 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
     1021 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
     1081 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
     1141 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     1201 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     1261 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     1321 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     1381 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     1441 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     1501 gaagcggccc tgcagatatc aacaagtttg tacaaaaaag caggcaccat gcgaccctcc
     1561 gggacggccg gggcagcgct cctggcgctg ctggctgcgc tctgcccggc gagtcgggct
     1621 ctggaggaaa agaaagtttg ccaaggcacg agtaacaagc tcacgcagtt gggcactttt
     1681 gaagatcatt ttctcagcct ccagaggatg ttcaataact gtgaggtggt ccttgggaat
     1741 ttggaaatta cctatgtgca gaggaattat gatctttcct tcttaaagac catccaggag
     1801 gtggctggtt atgtcctcat tgccctcaac acagtggagc gaattccttt ggaaaacctg
     1861 cagatcatca gaggaaatat gtactacgaa aattcctatg ccttagcagt cttatctaac
     1921 tatgatgcaa ataaaaccgg actgaaggag ctgcccatga gaaatttaca ggaaatcctg
     1981 catggcgccg tgcggttcag caacaaccct gccctgtgca acgtggagag catccagtgg
     2041 cgggacatag tcagcagtga ctttctcagc aacatgtcga tggacttcca gaaccacctg
     2101 ggcagctgcc aaaagtgtga tccaagctgt cccaatggga gctgctgggg tgcaggagag
     2161 gagaactgcc agaaactgac caaaatcatc tgtgcccagc agtgctccgg gcgctgccgt
     2221 ggcaagtccc ccagtgactg ctgccacaac cagtgtgctg caggctgcac aggcccccgg
     2281 gagagcgact gcctggtctg ccgcaaattc cgagacgaag ccacgtgcaa ggacacctgc
     2341 cccccactca tgctctacaa ccccaccacg taccagatgg atgtgaaccc cgagggcaaa
     2401 tacagctttg gtgacacctg cgtgaagaag tgtccccgta attatgtggt gacagatcac
     2461 ggctcgtgcg tccgagcctg tggggccgac agctatgaga tggaggaaga cggcgtccgc
     2521 aagtgtaaga agtgcgaagg gccttgccgc aaagtgtgta acggaatagg tattggtgaa
     2581 tttaaagact cactctccat aaatgctacg aatattaaac acttcaaaaa ctgcacctcc
     2641 atcagtggcg atctccacat cctgccggtg gcatttaggg gtgactcctt cacacatact
     2701 cctcctctgg atccacagga actggatatt ctgaaaaccg taaaggaaat cacagggttt
     2761 ttgctgattc aggcttggcc tgaaaacagg acggacctcc atgcctttga gaacctagaa
     2821 atcatacgcg gcaggaccaa gcaacatggt cagttttctc ttgcagtcgt cagcctgaac
     2881 ataacatcct tgggattacg ctccctcaag gagataagtg atggagatgt gataatttca
     2941 ggaaacaaaa atttgtgcta tgcaaataca ataaactgga aaaaactgtt tgggacctcc
     3001 ggtcagaaaa ccaaaattat aagcaacaga ggtgaaaaca gctgcaaggc cacaggccag
     3061 gtctgccatg ccttgtgctc ccccgagggc tgctggggcc cggagcccag ggactgcgtc
     3121 tcttgccgga atgtcagccg aggcagggaa tgcgtggaca agtgcaacct tctggagggt
     3181 gagccaaggg agtttgtgga gaactctgag tgcatacagt gccacccaga gtgcctgcct
     3241 caggccatga acatcacctg cacaggacgg ggaccagaca actgtatcca gtgtgcccac
     3301 tacattgacg gcccccactg cgtcaagacc tgcccggcag gagtcatggg agaaaacaac
     3361 accctggtct ggaagtacgc agacgccggc catgtgtgcc acctgtgcca tccaaactgc
     3421 acctacggat gcactgggcc aggtcttgaa ggctgtccaa cgaatgggcc taagatcccg
     3481 tccatcgcca ctgggatggt gggggccctc ctcttgctgc tggtggtggc cctggggatc
     3541 ggcctcttca tgcgaaggcg ccacatcgtt cggaagcgca cgctgcggag gctgctgcag
     3601 gagagggagc ttgtggagcc tcttacaccc agtggagaag ctcccaacca agctctcttg
     3661 aggatcttga aggaaactga attcaaaaag atcaaagtgc tgggctccgg tgcgttcggc
     3721 acggtgtata agggactctg gatcccagaa ggtgagaaag ttaaaattcc cgtcgctatc
     3781 aaggaattaa gagaagcaac atctccgaaa gccaacaagg aaatcctcga tgaagcctac
     3841 gtgatggcca gcgtggacaa cccccacgtg tgccgcctgc tgggcatctg cctcacctcc
     3901 accgtgcagc tcatcacgca gctcatgccc ttcggctgcc tcctggacta tgtccgggaa
     3961 cacaaagaca atattggctc ccagtacctg ctcaactggt gtgtgcagat cgcaaagggc
     4021 atgaactact tggaggaccg tcgcttggtg caccgcgacc tggcagccag gaacgtactg
     4081 gtgaaaacac cgcagcatgt caagatcaca gattttgggc tggccaaact gctgggtgcg
     4141 gaagagaaag aataccatgc agaaggaggc aaagtgccta tcaagtggat ggcattggaa
     4201 tcaattttac acagaatcta tacccaccag agtgatgtct ggagctacgg ggtgaccgtt
     4261 tgggagttga tgacctttgg atccaagcca tatgacggaa tccctgccag cgagatctcc
     4321 tccatcctgg agaaaggaga acgcctccct cagccaccca tatgtaccat cgatgtctac
     4381 atgatcatgg tcaagtgctg gatgatagac gcagatagtc gcccaaagtt ccgtgagttg
     4441 atcatcgaat tctccaaaat ggcccgagac ccccagcgct accttgtcat tcagggggat
     4501 gaaagaatgc atttgccaag tcctacagac tccaacttct accgtgccct gatggatgaa
     4561 gaagacatgg acgacgtggt ggatgccgac gagtacctca tcccacagca gggcttcttc
     4621 agcagcccct ccacgtcacg gactcccctc ctgagctctc tgagtgcaac cagcaacaat
     4681 tccaccgtgg cttgcattga tagaaatggg ctgcaaagct gtcccatcaa ggaagacagc
     4741 ttcttgcagc gatacagctc agaccccaca ggcgccttga ctgaggacag catagacgac
     4801 accttcctcc cagtgcctga atacataaac cagtccgttc ccaaaaggcc cgctggctct
     4861 gtgcagaatc ctgtctatca caatcagcct ctgaaccccg cgcccagcag agacccacac
     4921 taccaggacc cccacagcac tgcagtgggc aaccccgagt atctcaacac tgtccagccc
     4981 acctgtgtca acagcacatt cgacagccct gcccactggg cccagaaagg cagccaccaa
     5041 attagcctgg acaaccctga ctaccagcag gacttctttc ccaaggaagc caagccaaat
     5101 ggcatcttta agggctccac agctgaaaat gcagaatacc taagggtcgc gccacaaagc
     5161 agtgaattta ttggagcata gaacccagct ttcttgtaca aagtggttga tatccagcac
     5221 agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct
     5281 ctcctcggtc tcgattctac gcgtaccggt tagtaatgag atccctcccc cccccctaac
     5341 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     5401 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     5461 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     5521 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     5581 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     5641 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     5701 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     5761 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     5821 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     5881 cgatgataat atggccacac atatggtgag caagggcgag gagctgttca ccggggtggt
     5941 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     6001 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     6061 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     6121 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     6181 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     6241 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     6301 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     6361 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     6421 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     6481 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     6541 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     6601 catggacgag ctgtacaagt aaatcgatag atcctaatca acctctggat tacaaaattt
     6661 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6721 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6781 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6841 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6901 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6961 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     7021 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     7081 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     7141 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     7201 tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt acaaggcagc
     7261 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7321 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7381 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7441 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7501 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7561 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt taattaagga
     7621 aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     7681 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     7741 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7801 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7861 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7921 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7981 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8041 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     8101 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8161 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8221 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8281 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8341 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8401 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8461 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8521 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8581 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8641 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8701 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8761 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8821 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8881 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8941 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9001 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9061 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9121 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9181 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     9241 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     9301 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9361 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     9421 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     9481 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     9541 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     9601 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     9661 cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt
     9721 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9781 aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg
     9841 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     9901 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     9961 aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt
    10021 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
    10081 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
    10141 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    10201 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
    10261 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
    10321 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
    10381 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
    10441 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
    10501 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
    10561 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
    10621 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
    10681 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    10741 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    10801 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10861 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    10921 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    10981 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    11041 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    11101 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    11161 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    11221 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    11281 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    11341 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
    11401 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
    11461 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    11521 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    11581 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    11641 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    11701 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    11761 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    11821 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    11881 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    11941 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    12001 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    12061 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    12121 atagagttag gcagggatat tcacc
//
