LOCUS       sequence-250468-       13375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ALK R401Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13375)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 13375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ALK-R401Q
FEATURES             Location/Qualifiers
     source          1..13375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4911..4935)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4988..5029
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5050..5617
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5067..5619
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5211..5228)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5438..5457
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5631..5633,5634..5636,5637..6350)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5631..5633,5634..5636,5637..6347)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5676..5697)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5937..5956)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6284..6305
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6364..6952
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6417..6437)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6835..6846)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7027..7260
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7379..7397)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7465..7569
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7570..7638,7639..8430)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7788..7807)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8601..9189
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9090..9109
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9343..9360
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9600..9621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9636..9641,9642..9659,9660..9666)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9674..9690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9679..9701
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9698..9714
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9698..9714
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9764..9783
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9975..10608
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10655..10780
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11277..11510
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11695..11739
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12032..12149
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        12197..13375
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          12428..13366
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     13323..13343
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggcaccatgg gagccatcgg
       61 gctcctgtgg ctcctgccgc tgctgctttc cacggcagct gtgggctccg ggatggggac
      121 cggccagcgc gcgggctccc cagctgcggg gccgccgctg cagccccggg agccactcag
      181 ctactcgcgc ctgcagagga agagtctggc agttgacttc gtggtgccct cgctcttccg
      241 tgtctacgcc cgggacctac tgctgccacc atcctcctcg gagctgaagg ctggcaggcc
      301 cgaggcccgc ggctcgctag ctctggactg cgccccgctg ctcaggttgc tggggccggc
      361 gccgggggtc tcctggaccg ccggttcacc agccccggca gaggcccgga cgctgtccag
      421 ggtgctgaag ggcggctccg tgcgcaagct ccggcgtgcc aagcagttgg tgctggagct
      481 gggcgaggag gcgatcttgg agggttgcgt cgggcccccc ggggaggcgg ctgtggggct
      541 gctccagttc aatctcagcg agctgttcag ttggtggatt cgccaaggcg aagggcgact
      601 gaggatccgc ctgatgcccg agaagaaggc gtcggaagtg ggcagagagg gaaggctgtc
      661 cgcggcaatt cgcgcctccc agccccgcct tctcttccag atcttcggga ctggtcatag
      721 ctccttggaa tcaccaacaa acatgccttc tccttctcct gattatttta catggaatct
      781 cacctggata atgaaagact ccttcccttt cctgtctcat cgcagccgat atggtctgga
      841 gtgcagcttt gacttcccct gtgagctgga gtattcccct ccactgcatg acctcaggaa
      901 ccagagctgg tcctggcgcc gcatcccctc cgaggaggcc tcccagatgg acttgctgga
      961 tgggcctggg gcagagcgtt ctaaggagat gcccagaggc tcctttctcc ttctcaacac
     1021 ctcagctgac tccaagcaca ccatcctgag tccgtggatg aggagcagca gtgagcactg
     1081 cacactggcc gtctcggtgc acaggcacct gcagccctct ggaaggtaca ttgcccagct
     1141 gctgccccac aacgaggctg caagagagat cctcctgatg cccactccag ggaagcatgg
     1201 ttggacagtg ctccagggaa gaatcgggcg tccagacaac ccatttcaag tggccctgga
     1261 atacatctcc agtggaaacc gcagcttgtc tgcagtggac ttctttgccc tgaagaactg
     1321 cagtgaagga acatccccag gctccaagat ggccctgcag agctccttca cttgttggaa
     1381 tgggacagtc ctccagcttg ggcaggcctg tgacttccac caggactgtg cccagggaga
     1441 agatgagagc cagatgtgcc ggaaactgcc tgtgggtttt tactgcaact ttgaagatgg
     1501 cttctgtggc tggacccaag gcacactgtc accccacact cctcaatggc aggtcaggac
     1561 cctaaaggat gcccggttcc aggaccacca agaccatgct ctattgctca gtaccactga
     1621 tgtccccgct tctgaaagtg ctacagtgac cagtgctacg tttcctgcac cgatcaagag
     1681 ctctccatgt gagctccgaa tgtcctggct cattcgtgga gtcttgaggg gaaacgtgtc
     1741 cttggtgcta gtggagaaca aaaccgggaa ggagcaaggc aggatggtct ggcatgtcgc
     1801 cgcctatgaa ggcttgagcc tgtggcagtg gatggtgttg cctctcctcg atgtgtctga
     1861 caggttctgg ctgcagatgg tcgcatggtg gggacaagga tccagagcca tcgtggcttt
     1921 tgacaatatc tccatcagcc tggactgcta cctcaccatt agcggagagg acaagatcct
     1981 gcagaataca gcacccaaat caagaaacct gtttgagaga aacccaaaca aggagctgaa
     2041 acccggggaa aattcaccaa gacagacccc catctttgac cctacagttc attggctgtt
     2101 caccacatgt ggggccagcg ggccccatgg ccccacccag gcacagtgca acaacgccta
     2161 ccagaactcc aacctgagcg tggaggtggg gagcgagggc cccctgaaag gcatccagat
     2221 ctggaaggtg ccagccaccg acacctacag catctcgggc tacggagctg ctggcgggaa
     2281 aggcgggaag aacaccatga tgcggtccca cggcgtgtct gtgctgggca tcttcaacct
     2341 ggagaaggat gacatgctgt acatcctggt tgggcagcag ggagaggacg cctgccccag
     2401 tacaaaccag ttaatccaga aagtctgcat tggagagaac aatgtgatag aagaagaaat
     2461 ccgtgtgaac agaagcgtgc atgagtgggc aggaggcgga ggaggagggg gtggagccac
     2521 ctacgtattt aagatgaagg atggagtgcc ggtgcccctg atcattgcag ccggaggtgg
     2581 tggcagggcc tacggggcca agacagacac gttccaccca gagagactgg agaataactc
     2641 ctcggttcta gggctaaacg gcaattccgg agccgcaggt ggtggaggtg gctggaatga
     2701 taacacttcc ttgctctggg ccggaaaatc tttgcaggag ggtgccaccg gaggacattc
     2761 ctgcccccag gccatgaaga agtgggggtg ggagacaaga gggggtttcg gagggggtgg
     2821 aggggggtgc tcctcaggtg gaggaggcgg aggatatata ggcggcaatg cagcctcaaa
     2881 caatgacccc gaaatggatg gggaagatgg ggtttccttc atcagtccac tgggcatcct
     2941 gtacacccca gctttaaaag tgatggaagg ccacggggaa gtgaatatta agcattatct
     3001 aaactgcagt cactgtgagg tagacgaatg tcacatggac cctgaaagcc acaaggtcat
     3061 ctgcttctgt gaccacggga cggtgctggc tgaggatggc gtctcctgca ttgtgtcacc
     3121 caccccggag ccacacctgc cactctcgct gatcctctct gtggtgacct ctgccctcgt
     3181 ggccgccctg gtcctggctt tctccggcat catgattgtg taccgccgga agcaccagga
     3241 gctgcaagcc atgcagatgg agctgcagag ccctgagtac aagctgagca agctccgcac
     3301 ctcgaccatc atgaccgact acaaccccaa ctactgcttt gctggcaaga cctcctccat
     3361 cagtgacctg aaggaggtgc cgcggaaaaa catcaccctc attcggggtc tgggccatgg
     3421 cgcctttggg gaggtgtatg aaggccaggt gtccggaatg cccaacgacc caagccccct
     3481 gcaagtggct gtgaagacgc tgcctgaagt gtgctctgaa caggacgaac tggatttcct
     3541 catggaagcc ctgatcatca gcaaattcaa ccaccagaac attgttcgct gcattggggt
     3601 gagcctgcaa tccctgcccc ggttcatcct gctggagctc atggcggggg gagacctcaa
     3661 gtccttcctc cgagagaccc gccctcgccc gagccagccc tcctccctgg ccatgctgga
     3721 ccttctgcac gtggctcggg acattgcctg tggctgtcag tatttggagg aaaaccactt
     3781 catccaccga gacattgctg ccagaaactg cctcttgacc tgtccaggcc ctggaagagt
     3841 ggccaagatt ggagacttcg ggatggcccg agacatctac agggcgagct actatagaaa
     3901 gggaggctgt gccatgctgc cagttaagtg gatgccccca gaggccttca tggaaggaat
     3961 attcacttct aaaacagaca catggtcctt tggagtgctg ctatgggaaa tcttttctct
     4021 tggatatatg ccatacccca gcaaaagcaa ccaggaagtt ctggagtttg tcaccagtgg
     4081 aggccggatg gacccaccca agaactgccc tgggcctgta taccggataa tgactcagtg
     4141 ctggcaacat cagcctgaag acaggcccaa ctttgccatc attttggaga ggattgaata
     4201 ctgcacccag gacccggatg taatcaacac cgctttgccg atagaatatg gtccacttgt
     4261 ggaagaggaa gagaaagtgc ctgtgaggcc caaggaccct gagggggttc ctcctctcct
     4321 ggtctctcaa caggcaaaac gggaggagga gcgcagccca gctgccccac cacctctgcc
     4381 taccacctcc tctggcaagg ctgcaaagaa acccacagct gcagagatct ctgttcgagt
     4441 ccctagaggg ccggccgtgg aagggggaca cgtgaatatg gcattctctc agtccaaccc
     4501 tccttcggag ttgcacaagg tccacggatc cagaaacaag cccaccagct tgtggaaccc
     4561 aacgtacggc tcctggttta cagagaaacc caccaaaaag aataatccta tagcaaagaa
     4621 ggagccacac gacaggggta acctggggct ggagggaagc tgtactgtcc cacctaacgt
     4681 tgcaactggg agacttccgg gggcctcact gctcctagag ccctcttcgc tgactgccaa
     4741 tatgaaggag gtacctctgt tcaggctacg tcacttccct tgtgggaatg tcaattacgg
     4801 ctaccagcaa cagggcttgc ccttagaagc cgctactgcc cctggagctg gtcattacga
     4861 ggataccatt ctgaaaagca agaatagcat gaaccagcct gggccctaga acccagcttt
     4921 cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg
     4981 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta
     5041 gtaatgagat ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     5101 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     5161 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     5221 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     5281 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     5341 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     5401 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     5461 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     5521 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     5581 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacacat atggtgagca
     5641 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     5701 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     5761 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     5821 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     5881 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     5941 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     6001 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     6061 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     6121 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     6181 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     6241 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     6301 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa atcgatagat
     6361 cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6421 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6481 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6541 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6601 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6661 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6721 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6781 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6841 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6901 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc
     6961 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7021 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     7081 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7141 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7201 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7261 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7321 agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc
     7381 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     7441 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7501 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7561 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7621 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7681 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7741 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7801 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7861 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7921 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7981 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8041 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8101 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8161 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8221 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8281 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8341 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8401 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8461 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8521 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8581 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8641 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8701 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8761 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8821 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8881 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8941 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9001 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9061 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9121 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9181 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9241 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9301 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9361 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9421 tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     9481 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     9541 agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     9601 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     9661 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt
     9721 acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9781 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     9841 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     9901 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     9961 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
    10021 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
    10081 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
    10141 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
    10201 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
    10261 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
    10321 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
    10381 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
    10441 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
    10501 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
    10561 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
    10621 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
    10681 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
    10741 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
    10801 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
    10861 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
    10921 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
    10981 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
    11041 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
    11101 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
    11161 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    11221 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    11281 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    11341 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    11401 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    11461 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    11521 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    11581 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    11641 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    11701 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    11761 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    11821 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    11881 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    11941 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc
    12001 cgaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    12061 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    12121 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
    12181 tttggactag tcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
    12241 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
    12301 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
    12361 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
    12421 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
    12481 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
    12541 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
    12601 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
    12661 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
    12721 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
    12781 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
    12841 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
    12901 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
    12961 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
    13021 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
    13081 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
    13141 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
    13201 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
    13261 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
    13321 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtga
//
