LOCUS       sequence-250473-       10304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ARAF P216L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10304)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ARAF-P216L
FEATURES             Location/Qualifiers
     source          1..10304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..1226
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          279..1217
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1174..1194
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1246..1270
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3089..3113)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3121..3139
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3164..3183)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3165..3183)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3189..3209)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3263..3304
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3325..3892
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3342..3894
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3486..3503)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3713..3732
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3906..4505
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3906..3925)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4402..4422
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4519..5107
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4572..4592)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4990..5001)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5182..5415
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5534..5552)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5620..5724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5725..5793,5794..6585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5943..5962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6756..7344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7245..7264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7498..7515
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7755..7776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7791..7796,7797..7814,7815..7821)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7829..7845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7834..7856
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7853..7869
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7853..7869
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7919..7938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8130..8763
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8810..8935
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9432..9665
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9850..9894
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10187..10304
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgggtttatt acagggacag cagagatcca gtttggacta gtcgtgaggc tccggtgccc
       61 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      121 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      181 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      241 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
      301 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
      361 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
      421 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
      481 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
      541 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
      601 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
      661 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
      721 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
      781 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
      841 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
      901 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
      961 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     1021 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     1081 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     1141 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     1201 tttttcttcc atttcaggtg tcgtgaagcg gccctgcaga tatcaacaag tttgtacaaa
     1261 aaagttggca tggagccacc acggggcccc cctgccaatg gggccgagcc atcccgggca
     1321 gtgggcaccg tcaaagtata cctgcccaac aagcaacgca cggtggtgac tgtccgggat
     1381 ggcatgagtg tctacgactc tctagacaag gccctgaagg tgcggggtct aaatcaggac
     1441 tgctgtgtgg tctaccgact catcaaggga cgaaagacgg tcactgcctg ggacacagcc
     1501 attgctcccc tggatggcga ggagctcatt gtcgaggtcc ttgaagatgt cccgctgacc
     1561 atgcacaatt ttgtacggaa gaccttcttc agcctggcgt tctgtgactt ctgccttaag
     1621 tttctgttcc atggcttccg ttgccaaacc tgtggctaca agttccacca gcattgttcc
     1681 tccaaggtcc ccacagtctg tgttgacatg agtaccaacc gccaacagtt ctaccacagt
     1741 gtccaggatt tgtccggagg ctccagacag catgaggctc cctcgaaccg ccccctgaat
     1801 gagttgctaa ccccccaggg tcccagcccc cgcacccagc actgtgaccc ggagcacttc
     1861 cccttccctg ccccagccaa tgccccccta cagcgcatcc gctccacgtc cactctcaac
     1921 gtccatatgg tcagcaccac ggcccccatg gactccaacc tcatccagct cactggccag
     1981 agtttcagca ctgatgctgc cggtagtaga ggaggtagtg atggaacccc ccgggggagc
     2041 cccagcccag ccagcgtgtc ctcggggagg aagtccccac attccaagtc accagcagag
     2101 cagcgcgagc ggaagtcctt ggccgatgac aagaagaaag tgaagaacct ggggtaccgg
     2161 gactcaggct attactggga ggtaccaccc agtgaggtgc agctgctgaa gaggatcggg
     2221 acgggctcgt ttggcaccgt gtttcgaggg cggtggcatg gcgatgtggc cgtgaaggtg
     2281 ctcaaggtgt cccagcccac agctgagcag gcccaggctt tcaagaatga gatgcaggtg
     2341 ctcaggaaga cgcgacatgt caacatcttg ctgtttatgg gcttcatgac ccggccggga
     2401 tttgccatca tcacacagtg gtgtgagggc tccagcctct accatcacct gcatgtggcc
     2461 gacacacgct tcgacatggt ccagctcatc gacgtggccc ggcagactgc ccagggcatg
     2521 gactacctcc atgccaagaa catcatccac cgagatctca agtctaacaa catcttccta
     2581 catgaggggc tcacggtgaa gatcggtgac tttggcttgg ccacagtgaa gactcgatgg
     2641 agcggggccc agcccttgga gcagccctca ggatctgtgc tgtggatggc agctgaggtg
     2701 atccgtatgc aggacccgaa cccctacagc ttccagtcag acgtctatgc ctacggggtt
     2761 gtgctctacg agcttatgac tggctcactg ccttacagcc acattggctg ccgtgaccag
     2821 attatcttta tggtgggccg tggctatctg tccccggacc tcagcaaaat ctccagcaac
     2881 tgccccaagg ccatgcggcg cctgctgtct gactgcctca agttccagcg ggaggagcgg
     2941 cccctcttcc cccagatcct ggccacaatt gagctgctgc aacggtcact ccccaagatt
     3001 gagcggagtg cctcggaacc ctccttgcac cgcacccagg ccgatgagtt gcctgcctgc
     3061 ctactcagcg cagcccgcct tgtgccttac ccaactttct tgtacaaagt ggtgatatcc
     3121 aattaaccct cactaaaggg tgattgaata gtagattgtt taaccctata gtgagtcgta
     3181 ttaccaccca acttttctat acaaagtggt tgatatccag cacagtggcg gccgctcgag
     3241 tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc
     3301 tacgcgtacc ggttagtaat gagatccctc ccccccccct aacgttactg gccgaagccg
     3361 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     3421 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     3481 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     3541 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     3601 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     3661 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     3721 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     3781 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     3841 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     3901 cacatatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     3961 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     4021 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     4081 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     4141 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     4201 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     4261 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     4321 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     4381 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     4441 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     4501 cctaaatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4561 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4621 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4681 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4741 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4801 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4861 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     4921 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4981 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5041 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5101 tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat cttagccact
     5161 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     5221 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     5281 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5341 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     5401 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     5461 agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct
     5521 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5581 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5641 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5701 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5761 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5821 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5881 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5941 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     6001 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6061 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6121 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6181 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6241 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6301 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6361 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6421 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6481 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6541 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6601 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6661 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6721 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6781 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6841 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6901 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6961 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7021 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7081 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7141 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7201 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7261 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7321 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7381 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7441 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7501 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     7561 tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg
     7621 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7681 taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt
     7741 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7801 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7861 agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7921 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
     7981 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
     8041 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
     8101 tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca
     8161 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     8221 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     8281 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     8341 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     8401 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     8461 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     8521 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     8581 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8641 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8701 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8761 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     8821 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     8881 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     8941 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     9001 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     9061 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     9121 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     9181 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     9241 gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga
     9301 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     9361 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     9421 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     9481 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     9541 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     9601 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     9661 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     9721 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     9781 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     9841 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     9901 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     9961 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
    10021 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
    10081 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
    10141 tctcgacggt atcgccgaat tcacaaatgg cagtattcat ccacaatttt aaaagaaaag
    10201 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    10261 aaactaaaga attacaaaaa caaattacaa aaattcaaaa tttt
//
