LOCUS       sequence-250475-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR A611T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-A611T
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    132..249
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        297..1475
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          528..1466
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1423..1443
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1495..1519
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1522..1593,1594..3456,3457..3525,3526..5154)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYTDAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5156..5180)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5188..5206
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5231..5250)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5232..5250)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5256..5276)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5330..5371
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5392..5959
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5409..5961
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5553..5570)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5780..5799
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5973..6572
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5973..5992)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6469..6489
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6586..7174
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6639..6659)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7057..7068)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7249..7482
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7601..7619)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7687..7791
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7792..7860,7861..8652)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8823..9411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9312..9331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9565..9582
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9822..9843
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9858..9863,9864..9881,9882..9888)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9896..9912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9901..9923
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9920..9936
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9920..9936
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9986..10005
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10197..10830
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10877..11002
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11499..11732
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11917..11961
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
       61 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca aatggcagta
      121 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
      181 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
      241 caaaattttc gggtttatta cagggacagc agagatccag tttggactag tcgtgaggct
      301 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
      361 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
      421 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
      481 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
      541 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
      601 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
      661 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      721 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
      781 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
      841 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
      901 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
      961 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     1021 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     1081 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     1141 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     1201 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     1261 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1321 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1381 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1441 ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccctgcagat atcaacaagt
     1501 ttgtacaaaa aagcaggcac catgcgaccc tccgggacgg ccggggcagc gctcctggcg
     1561 ctgctggctg cgctctgccc ggcgagtcgg gctctggagg aaaagaaagt ttgccaaggc
     1621 acgagtaaca agctcacgca gttgggcact tttgaagatc attttctcag cctccagagg
     1681 atgttcaata actgtgaggt ggtccttggg aatttggaaa ttacctatgt gcagaggaat
     1741 tatgatcttt ccttcttaaa gaccatccag gaggtggctg gttatgtcct cattgccctc
     1801 aacacagtgg agcgaattcc tttggaaaac ctgcagatca tcagaggaaa tatgtactac
     1861 gaaaattcct atgccttagc agtcttatct aactatgatg caaataaaac cggactgaag
     1921 gagctgccca tgagaaattt acaggaaatc ctgcatggcg ccgtgcggtt cagcaacaac
     1981 cctgccctgt gcaacgtgga gagcatccag tggcgggaca tagtcagcag tgactttctc
     2041 agcaacatgt cgatggactt ccagaaccac ctgggcagct gccaaaagtg tgatccaagc
     2101 tgtcccaatg ggagctgctg gggtgcagga gaggagaact gccagaaact gaccaaaatc
     2161 atctgtgccc agcagtgctc cgggcgctgc cgtggcaagt cccccagtga ctgctgccac
     2221 aaccagtgtg ctgcaggctg cacaggcccc cgggagagcg actgcctggt ctgccgcaaa
     2281 ttccgagacg aagccacgtg caaggacacc tgccccccac tcatgctcta caaccccacc
     2341 acgtaccaga tggatgtgaa ccccgagggc aaatacagct ttggtgccac ctgcgtgaag
     2401 aagtgtcccc gtaattatgt ggtgacagat cacggctcgt gcgtccgagc ctgtggggcc
     2461 gacagctatg agatggagga agacggcgtc cgcaagtgta agaagtgcga agggccttgc
     2521 cgcaaagtgt gtaacggaat aggtattggt gaatttaaag actcactctc cataaatgct
     2581 acgaatatta aacacttcaa aaactgcacc tccatcagtg gcgatctcca catcctgccg
     2641 gtggcattta ggggtgactc cttcacacat actcctcctc tggatccaca ggaactggat
     2701 attctgaaaa ccgtaaagga aatcacaggg tttttgctga ttcaggcttg gcctgaaaac
     2761 aggacggacc tccatgcctt tgagaaccta gaaatcatac gcggcaggac caagcaacat
     2821 ggtcagtttt ctcttgcagt cgtcagcctg aacataacat ccttgggatt acgctccctc
     2881 aaggagataa gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat
     2941 acaataaact ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat tataagcaac
     3001 agaggtgaaa acagctgcaa ggccacaggc caggtctgcc atgccttgtg ctcccccgag
     3061 ggctgctggg gcccggagcc cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg
     3121 gaatgcgtgg acaagtgcaa ccttctggag ggtgagccaa gggagtttgt ggagaactct
     3181 gagtgcatac agtgccaccc agagtgcctg cctcaggcca tgaacatcac ctgcacagga
     3241 cggggaccag acaactgtat ccagtgtgcc cactacattg acggccccca ctgcgtcaag
     3301 acctgcccgg caggagtcat gggagaaaac aacaccctgg tctggaagta cacagacgcc
     3361 ggccatgtgt gccacctgtg ccatccaaac tgcacctacg gatgcactgg gccaggtctt
     3421 gaaggctgtc caacgaatgg gcctaagatc ccgtccatcg ccactgggat ggtgggggcc
     3481 ctcctcttgc tgctggtggt ggccctgggg atcggcctct tcatgcgaag gcgccacatc
     3541 gttcggaagc gcacgctgcg gaggctgctg caggagaggg agcttgtgga gcctcttaca
     3601 cccagtggag aagctcccaa ccaagctctc ttgaggatct tgaaggaaac tgaattcaaa
     3661 aagatcaaag tgctgggctc cggtgcgttc ggcacggtgt ataagggact ctggatccca
     3721 gaaggtgaga aagttaaaat tcccgtcgct atcaaggaat taagagaagc aacatctccg
     3781 aaagccaaca aggaaatcct cgatgaagcc tacgtgatgg ccagcgtgga caacccccac
     3841 gtgtgccgcc tgctgggcat ctgcctcacc tccaccgtgc agctcatcac gcagctcatg
     3901 cccttcggct gcctcctgga ctatgtccgg gaacacaaag acaatattgg ctcccagtac
     3961 ctgctcaact ggtgtgtgca gatcgcaaag ggcatgaact acttggagga ccgtcgcttg
     4021 gtgcaccgcg acctggcagc caggaacgta ctggtgaaaa caccgcagca tgtcaagatc
     4081 acagattttg ggctggccaa actgctgggt gcggaagaga aagaatacca tgcagaagga
     4141 ggcaaagtgc ctatcaagtg gatggcattg gaatcaattt tacacagaat ctatacccac
     4201 cagagtgatg tctggagcta cggggtgacc gtttgggagt tgatgacctt tggatccaag
     4261 ccatatgacg gaatccctgc cagcgagatc tcctccatcc tggagaaagg agaacgcctc
     4321 cctcagccac ccatatgtac catcgatgtc tacatgatca tggtcaagtg ctggatgata
     4381 gacgcagata gtcgcccaaa gttccgtgag ttgatcatcg aattctccaa aatggcccga
     4441 gacccccagc gctaccttgt cattcagggg gatgaaagaa tgcatttgcc aagtcctaca
     4501 gactccaact tctaccgtgc cctgatggat gaagaagaca tggacgacgt ggtggatgcc
     4561 gacgagtacc tcatcccaca gcagggcttc ttcagcagcc cctccacgtc acggactccc
     4621 ctcctgagct ctctgagtgc aaccagcaac aattccaccg tggcttgcat tgatagaaat
     4681 gggctgcaaa gctgtcccat caaggaagac agcttcttgc agcgatacag ctcagacccc
     4741 acaggcgcct tgactgagga cagcatagac gacaccttcc tcccagtgcc tgaatacata
     4801 aaccagtccg ttcccaaaag gcccgctggc tctgtgcaga atcctgtcta tcacaatcag
     4861 cctctgaacc ccgcgcccag cagagaccca cactaccagg acccccacag cactgcagtg
     4921 ggcaaccccg agtatctcaa cactgtccag cccacctgtg tcaacagcac attcgacagc
     4981 cctgcccact gggcccagaa aggcagccac caaattagcc tggacaaccc tgactaccag
     5041 caggacttct ttcccaagga agccaagcca aatggcatct ttaagggctc cacagctgaa
     5101 aatgcagaat acctaagggt cgcgccacaa agcagtgaat ttattggagc atagaaccca
     5161 gctttcttgt acaaagtggt gatatccaat taaccctcac taaaggtatt agagtttgag
     5221 aataagtagt ccctatagtg agtcgtatta ccacccaact tttctataca aagtggttga
     5281 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     5341 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag atccctcccc
     5401 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     5461 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     5521 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     5581 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     5641 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     5701 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     5761 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     5821 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     5881 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     5941 ttgaaaaaca cgatgataat atggccacac atatgaccga gtacaagccc acggtgcgcc
     6001 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     6061 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     6121 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6181 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6241 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6301 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     6361 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     6421 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     6481 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     6541 cctggtgcat gacccgcaag cccggtgcct aaatcgatag atcctaatca acctctggat
     6601 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6661 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6721 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6781 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6841 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6901 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6961 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7021 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7081 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7141 acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt
     7201 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7261 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7321 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7381 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7441 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     7501 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt
     7561 taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt
     7621 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7681 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7741 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7801 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7861 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7921 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7981 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     8041 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8101 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8161 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8221 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8281 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8341 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8401 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8461 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8521 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8581 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8641 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8701 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8761 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8821 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8881 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8941 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9001 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9061 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9121 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9181 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9241 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9301 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9361 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9421 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9481 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     9541 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     9601 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct
     9661 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     9721 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac
     9781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     9841 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     9901 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa
     9961 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    10021 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    10081 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    10141 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
    10201 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
    10261 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
    10321 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
    10381 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
    10441 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
    10501 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
    10561 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
    10621 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
    10681 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
    10741 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
    10801 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
    10861 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
    10921 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
    10981 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
    11041 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
    11101 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
    11161 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
    11221 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
    11281 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
    11341 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
    11401 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    11461 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    11521 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    11581 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    11641 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    11701 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    11761 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    11821 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    11881 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    11941 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    12001 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    12061 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    12121 ac
//
