LOCUS       sequence-250476-       10304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ARAF R188H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10304)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ARAF-R188H
FEATURES             Location/Qualifiers
     source          1..10304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3042..3066)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3074..3092
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3117..3136)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3118..3136)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3142..3162)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3216..3257
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3278..3845
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3295..3847
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3439..3456)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3666..3685
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3859..4458
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3859..3878)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4355..4375
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4472..5060
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4525..4545)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4943..4954)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5135..5368
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5487..5505)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5573..5677
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5678..5746,5747..6538)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5896..5915)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6709..7297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7198..7217
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7451..7468
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7708..7729
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7744..7749,7750..7767,7768..7774)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7782..7798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7787..7809
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7806..7822
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7806..7822
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7872..7891
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8083..8716
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8763..8888
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9385..9618
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9803..9847
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10140..10257
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccctgc agatatcaac
     1201 aagtttgtac aaaaaagttg gcatggagcc accacggggc ccccctgcca atggggccga
     1261 gccatcccgg gcagtgggca ccgtcaaagt atacctgccc aacaagcaac gcacggtggt
     1321 gactgtccgg gatggcatga gtgtctacga ctctctagac aaggccctga aggtgcgggg
     1381 tctaaatcag gactgctgtg tggtctaccg actcatcaag ggacgaaaga cggtcactgc
     1441 ctgggacaca gccattgctc ccctggatgg cgaggagctc attgtcgagg tccttgaaga
     1501 tgtcccgctg accatgcaca attttgtacg gaagaccttc ttcagcctgg cgttctgtga
     1561 cttctgcctt aagtttctgt tccatggctt ccgttgccaa acctgtggct acaagttcca
     1621 ccagcattgt tcctccaagg tccccacagt ctgtgttgac atgagtacca accgccaaca
     1681 gttctaccac agtgtccagg atttgtccgg aggctccaga cagcatgagg ctccctcgaa
     1741 ccgccccctg aatgagttgc taacccccca gggtcccagc ccccacaccc agcactgtga
     1801 cccggagcac ttccccttcc ctgccccagc caatgccccc ctacagcgca tccgctccac
     1861 gtccactccc aacgtccata tggtcagcac cacggccccc atggactcca acctcatcca
     1921 gctcactggc cagagtttca gcactgatgc tgccggtagt agaggaggta gtgatggaac
     1981 cccccggggg agccccagcc cagccagcgt gtcctcgggg aggaagtccc cacattccaa
     2041 gtcaccagca gagcagcgcg agcggaagtc cttggccgat gacaagaaga aagtgaagaa
     2101 cctggggtac cgggactcag gctattactg ggaggtacca cccagtgagg tgcagctgct
     2161 gaagaggatc gggacgggct cgtttggcac cgtgtttcga gggcggtggc atggcgatgt
     2221 ggccgtgaag gtgctcaagg tgtcccagcc cacagctgag caggcccagg ctttcaagaa
     2281 tgagatgcag gtgctcagga agacgcgaca tgtcaacatc ttgctgttta tgggcttcat
     2341 gacccggccg ggatttgcca tcatcacaca gtggtgtgag ggctccagcc tctaccatca
     2401 cctgcatgtg gccgacacac gcttcgacat ggtccagctc atcgacgtgg cccggcagac
     2461 tgcccagggc atggactacc tccatgccaa gaacatcatc caccgagatc tcaagtctaa
     2521 caacatcttc ctacatgagg ggctcacggt gaagatcggt gactttggct tggccacagt
     2581 gaagactcga tggagcgggg cccagccctt ggagcagccc tcaggatctg tgctgtggat
     2641 ggcagctgag gtgatccgta tgcaggaccc gaacccctac agcttccagt cagacgtcta
     2701 tgcctacggg gttgtgctct acgagcttat gactggctca ctgccttaca gccacattgg
     2761 ctgccgtgac cagattatct ttatggtggg ccgtggctat ctgtccccgg acctcagcaa
     2821 aatctccagc aactgcccca aggccatgcg gcgcctgctg tctgactgcc tcaagttcca
     2881 gcgggaggag cggcccctct tcccccagat cctggccaca attgagctgc tgcaacggtc
     2941 actccccaag attgagcgga gtgcctcgga accctccttg caccgcaccc aggccgatga
     3001 gttgcctgcc tgcctactca gcgcagcccg ccttgtgcct tacccaactt tcttgtacaa
     3061 agtggtgata tccaattaac cctcactaaa ggaagatgat agttaagtgt aagttaccct
     3121 atagtgagtc gtattaccac ccaacttttc tatacaaagt ggttgatatc cagcacagtg
     3181 gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc
     3241 tcggtctcga ttctacgcgt accggttagt aatgagatcc ctcccccccc cctaacgtta
     3301 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     3361 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     3421 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     3481 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     3541 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     3601 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     3661 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     3721 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     3781 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     3841 gataatatgg ccacacatat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     3901 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     3961 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     4021 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     4081 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     4141 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     4201 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     4261 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     4321 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     4381 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     4441 cgcaagcccg gtgcctaaat cgatagatcc taatcaacct ctggattaca aaatttgtga
     4501 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4561 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4621 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4681 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4741 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4801 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4861 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4921 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4981 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5041 cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta
     5101 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5161 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     5221 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5281 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5341 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     5401 ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg
     5461 gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5521 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5581 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5641 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5701 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5761 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5821 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5881 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     5941 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6001 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6061 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6121 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6181 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6241 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6301 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6361 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6421 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6481 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6541 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6601 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6661 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6721 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6781 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6841 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6901 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6961 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7021 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7081 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7141 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7201 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7261 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7321 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7381 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7441 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7501 tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt
     7561 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7621 ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg
     7681 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     7741 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     7801 tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact
     7861 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     7921 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     7981 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     8041 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
     8101 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     8161 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     8221 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     8281 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     8341 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     8401 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     8461 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     8521 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8581 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8641 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8701 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     8761 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     8821 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     8881 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     8941 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     9001 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     9061 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     9121 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     9181 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     9241 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     9301 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     9361 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     9421 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     9481 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     9541 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     9601 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     9661 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     9721 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     9781 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     9841 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     9901 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     9961 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
    10021 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
    10081 attagtgaac ggatctcgac ggtatcgccg aattcacaaa tggcagtatt catccacaat
    10141 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
    10201 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
    10261 gtttattaca gggacagcag agatccagtt tggactagtc gtga
//
