LOCUS       sequence-250479-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR A702S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-A702S
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    197..314
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        362..1540
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          593..1531
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1488..1508
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1560..1584
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1587..1658,1659..3521,3522..3590,3591..5219)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQSLLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5221..5245)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5253..5271
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5296..5315)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5297..5315)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5321..5341)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5395..5436
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5457..6024
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5474..6026
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5618..5635)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5845..5864
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6038..6637
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6038..6057)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6534..6554
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6651..7239
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6704..6724)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7122..7133)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7314..7547
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7666..7684)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7752..7856
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7857..7925,7926..8717)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8888..9476
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9377..9396
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9630..9647
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9887..9908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9923..9928,9929..9946,9947..9953)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9961..9977
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9966..9988
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9985..10001
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9985..10001
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10051..10070
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10262..10895
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10942..11067
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11564..11797
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11982..12026
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
       61 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
      121 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat tcacaaatgg
      181 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
      241 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      301 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg actagtcgtg
      361 aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      421 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      481 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      541 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      601 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      661 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
      721 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
      781 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
      841 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
      901 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
      961 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     1021 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     1081 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     1141 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     1201 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     1261 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     1321 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     1381 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     1441 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     1501 agtggttcaa agtttttttc ttccatttca ggtgtcgtga agcggccctg cagatatcaa
     1561 caagtttgta caaaaaagca ggtaccatgc gaccctccgg gacggccggg gcagcgctcc
     1621 tggcgctgct ggctgcgctc tgcccggcga gtcgggctct ggaggaaaag aaagtttgcc
     1681 aaggcacgag taacaagctc acgcagttgg gcacttttga agatcatttt ctcagcctcc
     1741 agaggatgtt caataactgt gaggtggtcc ttgggaattt ggaaattacc tatgtgcaga
     1801 ggaattatga tctttccttc ttaaagacca tccaggaggt ggctggttat gtcctcattg
     1861 ccctcaacac agtggagcga attcctttgg aaaacctgca gatcatcaga ggaaatatgt
     1921 actacgaaaa ttcctatgcc ttagcagtct tatctaacta tgatgcaaat aaaaccggac
     1981 tgaaggagct gcccatgaga aatttacagg aaatcctgca tggcgccgtg cggttcagca
     2041 acaaccctgc cctgtgcaac gtggagagca tccagtggcg ggacatagtc agcagtgact
     2101 ttctcagcaa catgtcgatg gacttccaga accacctggg cagctgccaa aagtgtgatc
     2161 caagctgtcc caatgggagc tgctggggtg caggagagga gaactgccag aaactgacca
     2221 aaatcatctg tgcccagcag tgctccgggc gctgccgtgg caagtccccc agtgactgct
     2281 gccacaacca gtgtgctgca ggctgcacag gcccccggga gagcgactgc ctggtctgcc
     2341 gcaaattccg agacgaagcc acgtgcaagg acacctgccc cccactcatg ctctacaacc
     2401 ccaccacgta ccagatggat gtgaaccccg agggcaaata cagctttggt gccacctgcg
     2461 tgaagaagtg tccccgtaat tatgtggtga cagatcacgg ctcgtgcgtc cgagcctgtg
     2521 gggccgacag ctatgagatg gaggaagacg gcgtccgcaa gtgtaagaag tgcgaagggc
     2581 cttgccgcaa agtgtgtaac ggaataggta ttggtgaatt taaagactca ctctccataa
     2641 atgctacgaa tattaaacac ttcaaaaact gcacctccat cagtggcgat ctccacatcc
     2701 tgccggtggc atttaggggt gactccttca cacatactcc tcctctggat ccacaggaac
     2761 tggatattct gaaaaccgta aaggaaatca cagggttttt gctgattcag gcttggcctg
     2821 aaaacaggac ggacctccat gcctttgaga acctagaaat catacgcggc aggaccaagc
     2881 aacatggtca gttttctctt gcagtcgtca gcctgaacat aacatccttg ggattacgct
     2941 ccctcaagga gataagtgat ggagatgtga taatttcagg aaacaaaaat ttgtgctatg
     3001 caaatacaat aaactggaaa aaactgtttg ggacctccgg tcagaaaacc aaaattataa
     3061 gcaacagagg tgaaaacagc tgcaaggcca caggccaggt ctgccatgcc ttgtgctccc
     3121 ccgagggctg ctggggcccg gagcccaggg actgcgtctc ttgccggaat gtcagccgag
     3181 gcagggaatg cgtggacaag tgcaaccttc tggagggtga gccaagggag tttgtggaga
     3241 actctgagtg catacagtgc cacccagagt gcctgcctca ggccatgaac atcacctgca
     3301 caggacgggg accagacaac tgtatccagt gtgcccacta cattgacggc ccccactgcg
     3361 tcaagacctg cccggcagga gtcatgggag aaaacaacac cctggtctgg aagtacgcag
     3421 acgccggcca tgtgtgccac ctgtgccatc caaactgcac ctacggatgc actgggccag
     3481 gtcttgaagg ctgtccaacg aatgggccta agatcccgtc catcgccact gggatggtgg
     3541 gggccctcct cttgctgctg gtggtggccc tggggatcgg cctcttcatg cgaaggcgcc
     3601 acatcgttcg gaagcgcacg ctgcggaggc tgctgcagga gagggagctt gtggagcctc
     3661 ttacacccag tggagaagct cccaaccaat ctctcttgag gatcttgaag gaaactgaat
     3721 tcaaaaagat caaagtgctg ggctccggtg cgttcggcac ggtgtataag ggactctgga
     3781 tcccagaagg tgagaaagtt aaaattcccg tcgctatcaa ggaattaaga gaagcaacat
     3841 ctccgaaagc caacaaggaa atcctcgatg aagcctacgt gatggccagc gtggacaacc
     3901 cccacgtgtg ccgcctgctg ggcatctgcc tcacctccac cgtgcagctc atcacgcagc
     3961 tcatgccctt cggctgcctc ctggactatg tccgggaaca caaagacaat attggctccc
     4021 agtacctgct caactggtgt gtgcagatcg caaagggcat gaactacttg gaggaccgtc
     4081 gcttggtgca ccgcgacctg gcagccagga acgtactggt gaaaacaccg cagcatgtca
     4141 agatcacaga ttttgggctg gccaaactgc tgggtgcgga agagaaagaa taccatgcag
     4201 aaggaggcaa agtgcctatc aagtggatgg cattggaatc aattttacac agaatctata
     4261 cccaccagag tgatgtctgg agctacgggg tgaccgtttg ggagttgatg acctttggat
     4321 ccaagccata tgacggaatc cctgccagcg agatctcctc catcctggag aaaggagaac
     4381 gcctccctca gccacccata tgtaccatcg atgtctacat gatcatggtc aagtgctgga
     4441 tgatagacgc agatagtcgc ccaaagttcc gtgagttgat catcgaattc tccaaaatgg
     4501 cccgagaccc ccagcgctac cttgtcattc agggggatga aagaatgcat ttgccaagtc
     4561 ctacagactc caacttctac cgtgccctga tggatgaaga agacatggac gacgtggtgg
     4621 atgccgacga gtacctcatc ccacagcagg gcttcttcag cagcccctcc acgtcacgga
     4681 ctcccctcct gagctctctg agtgcaacca gcaacaattc caccgtggct tgcattgata
     4741 gaaatgggct gcaaagctgt cccatcaagg aagacagctt cttgcagcga tacagctcag
     4801 accccacagg cgccttgact gaggacagca tagacgacac cttcctccca gtgcctgaat
     4861 acataaacca gtccgttccc aaaaggcccg ctggctctgt gcagaatcct gtctatcaca
     4921 atcagcctct gaaccccgcg cccagcagag acccacacta ccaggacccc cacagcactg
     4981 cagtgggcaa ccccgagtat ctcaacactg tccagcccac ctgtgtcaac agcacattcg
     5041 acagccctgc ccactgggcc cagaaaggca gccaccaaat tagcctggac aaccctgact
     5101 accagcagga cttctttccc aaggaagcca agccaaatgg catctttaag ggctccacag
     5161 ctgaaaatgc agaataccta agggtcgcgc cacaaagcag tgaatttatt ggagcataga
     5221 acccagcttt cttgtacaaa gtggtgatat ccaattaacc ctcactaaag gagagtatta
     5281 gtagttattg taagtcccta tagtgagtcg tattaccacc caacttttct atacaaagtg
     5341 gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag
     5401 cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta atgagatccc
     5461 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     5521 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     5581 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     5641 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     5701 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     5761 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     5821 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     5881 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     5941 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     6001 ttcctttgaa aaacacgatg ataatatggc cacacatatg accgagtaca agcccacggt
     6061 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     6121 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     6181 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6241 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6301 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6361 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6421 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6481 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     6541 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6601 gcgcacctgg tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct aatcaacctc
     6661 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6721 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6781 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6841 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6901 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6961 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7021 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7081 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7141 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7201 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat
     7261 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7321 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     7381 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7441 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7501 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7561 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     7621 cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc
     7681 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     7741 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7801 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7861 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7921 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7981 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8041 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     8101 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8161 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8221 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8281 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8341 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8401 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8461 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8521 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8581 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8641 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8701 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8761 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8821 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8881 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8941 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9001 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9061 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9121 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9181 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9241 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9301 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9361 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9421 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9481 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9541 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9601 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9661 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca
     9721 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     9781 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc
     9841 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     9901 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     9961 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa
    10021 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10081 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    10141 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    10201 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    10261 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
    10321 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
    10381 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
    10441 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
    10501 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
    10561 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
    10621 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
    10681 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
    10741 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10801 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10861 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
    10921 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    10981 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    11041 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    11101 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    11161 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    11221 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    11281 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    11341 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    11401 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
    11461 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
    11521 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
    11581 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
    11641 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
    11701 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
    11761 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
    11821 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
    11881 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
    11941 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
    12001 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
    12061 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
    12121 ta
//
