LOCUS       sequence-250482-       12110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR A839V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12110)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-A839V
FEATURES             Location/Qualifiers
     source          1..12110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    208..325
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        373..1551
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          604..1542
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1499..1519
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1571..1595
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1598..1669,1670..3532,3533..3601,3602..5230)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLVARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5232..5256)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5264..5282
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5307..5326)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5308..5326)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5332..5352)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5406..5447
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5468..6035
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5485..6037
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5629..5646)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5856..5875
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6049..6648
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6049..6068)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6545..6565
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6662..7250
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6715..6735)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7133..7144)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7325..7558
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7677..7695)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7763..7867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7868..7936,7937..8728)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8899..9487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9388..9407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9641..9658
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9898..9919
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9934..9939,9940..9957,9958..9964)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9972..9988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9977..9999
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9996..10012
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9996..10012
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10062..10081
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10261..10894
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10941..11066
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11563..11796
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11981..12025
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
       61 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
      121 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa
      181 ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
      241 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
      301 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
      361 gactagtcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
      421 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
      481 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
      541 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
      601 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
      661 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
      721 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
      781 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
      841 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
      901 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
      961 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     1021 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     1081 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     1141 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     1201 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     1261 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     1321 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     1381 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     1441 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     1501 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct
     1561 gcagatatca acaagtttgt acaaaaaagc aggcaccatg cgaccctccg ggacggccgg
     1621 ggcagcgctc ctggcgctgc tggctgcgct ctgcccggcg agtcgggctc tggaggaaaa
     1681 gaaagtttgc caaggcacga gtaacaagct cacgcagttg ggcacttttg aagatcattt
     1741 tctcagcctc cagaggatgt tcaataactg tgaggtggtc cttgggaatt tggaaattac
     1801 ctatgtgcag aggaattatg atctttcctt cttaaagacc atccaggagg tggctggtta
     1861 tgtcctcatt gccctcaaca cagtggagcg aattcctttg gaaaacctgc agatcatcag
     1921 aggaaatatg tactacgaaa attcctatgc cttagcagtc ttatctaact atgatgcaaa
     1981 taaaaccgga ctgaaggagc tgcccatgag aaatttacag gaaatcctgc atggcgccgt
     2041 gcggttcagc aacaaccctg ccctgtgcaa cgtggagagc atccagtggc gggacatagt
     2101 cagcagtgac tttctcagca acatgtcgat ggacttccag aaccacctgg gcagctgcca
     2161 aaagtgtgat ccaagctgtc ccaatgggag ctgctggggt gcaggagagg agaactgcca
     2221 gaaactgacc aaaatcatct gtgcccagca gtgctccggg cgctgccgtg gcaagtcccc
     2281 cagtgactgc tgccacaacc agtgtgctgc aggctgcaca ggcccccggg agagcgactg
     2341 cctggtctgc cgcaaattcc gagacgaagc cacgtgcaag gacacctgcc ccccactcat
     2401 gctctacaac cccaccacgt accagatgga tgtgaacccc gagggcaaat acagctttgg
     2461 tgccacctgc gtgaagaagt gtccccgtaa ttatgtggtg acagatcacg gctcgtgcgt
     2521 ccgagcctgt ggggccgaca gctatgagat ggaggaagac ggcgtccgca agtgtaagaa
     2581 gtgcgaaggg ccttgccgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc
     2641 actctccata aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga
     2701 tctccacatc ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga
     2761 tccacaggaa ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca
     2821 ggcttggcct gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg
     2881 caggaccaag caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt
     2941 gggattacgc tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa
     3001 tttgtgctat gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac
     3061 caaaattata agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc
     3121 cttgtgctcc cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa
     3181 tgtcagccga ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga
     3241 gtttgtggag aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa
     3301 catcacctgc acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg
     3361 cccccactgc gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg
     3421 gaagtacgca gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg
     3481 cactgggcca ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac
     3541 tgggatggtg ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat
     3601 gcgaaggcgc cacatcgttc ggaagcgcac gctgcggagg ctgctgcagg agagggagct
     3661 tgtggagcct cttacaccca gtggagaagc tcccaaccaa gctctcttga ggatcttgaa
     3721 ggaaactgaa ttcaaaaaga tcaaagtgct gggctccggt gcgttcggca cggtgtataa
     3781 gggactctgg atcccagaag gtgagaaagt taaaattccc gtcgctatca aggaattaag
     3841 agaagcaaca tctccgaaag ccaacaagga aatcctcgat gaagcctacg tgatggccag
     3901 cgtggacaac ccccacgtgt gccgcctgct gggcatctgc ctcacctcca ccgtgcagct
     3961 catcacgcag ctcatgccct tcggctgcct cctggactat gtccgggaac acaaagacaa
     4021 tattggctcc cagtacctgc tcaactggtg tgtgcagatc gcaaagggca tgaactactt
     4081 ggaggaccgt cgcttggtgc accgcgacct ggtagccagg aacgtactgg tgaaaacacc
     4141 gcagcatgtc aagatcacag attttgggct ggccaaactg ctgggtgcgg aagagaaaga
     4201 ataccatgca gaaggaggca aagtgcctat caagtggatg gcattggaat caattttaca
     4261 cagaatctat acccaccaga gtgatgtctg gagctacggg gtgaccgttt gggagttgat
     4321 gacctttgga tccaagccat atgacggaat ccctgccagc gagatctcct ccatcctgga
     4381 gaaaggagaa cgcctccctc agccacccat atgtaccatc gatgtctaca tgatcatggt
     4441 caagtgctgg atgatagacg cagatagtcg cccaaagttc cgtgagttga tcatcgaatt
     4501 ctccaaaatg gcccgagacc cccagcgcta ccttgtcatt cagggggatg aaagaatgca
     4561 tttgccaagt cctacagact ccaacttcta ccgtgccctg atggatgaag aagacatgga
     4621 cgacgtggtg gatgccgacg agtacctcat cccacagcag ggcttcttca gcagcccctc
     4681 cacgtcacgg actcccctcc tgagctctct gagtgcaacc agcaacaatt ccaccgtggc
     4741 ttgcattgat agaaatgggc tgcaaagctg tcccatcaag gaagacagct tcttgcagcg
     4801 atacagctca gaccccacag gcgccttgac tgaggacagc atagacgaca ccttcctccc
     4861 agtgcctgaa tacataaacc agtccgttcc caaaaggccc gctggctctg tgcagaatcc
     4921 tgtctatcac aatcagcctc tgaaccccgc gcccagcaga gacccacact accaggaccc
     4981 ccacagcact gcagtgggca accccgagta tctcaacact gtccagccca cctgtgtcaa
     5041 cagcacattc gacagccctg cccactgggc ccagaaaggc agccaccaaa ttagcctgga
     5101 caaccctgac taccagcagg acttctttcc caaggaagcc aagccaaatg gcatctttaa
     5161 gggctccaca gctgaaaatg cagaatacct aagggtcgcg ccacaaagca gtgaatttat
     5221 tggagcatag aacccagctt tcttgtacaa agtggtgata tccaattaac cctcactaaa
     5281 ggatttatgt agttgagagt gataaaccct atagtgagtc gtattaccac ccaacttttc
     5341 tatacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt
     5401 tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt
     5461 aatgagatcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     5521 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     5581 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     5641 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     5701 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     5761 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     5821 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     5881 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     5941 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     6001 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacacatat gaccgagtac
     6061 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     6121 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6181 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6241 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6301 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6361 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6421 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6481 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6541 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6601 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctaaat cgatagatcc
     6661 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6721 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6781 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6841 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6901 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6961 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7021 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     7081 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7141 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7201 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct
     7261 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     7321 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     7381 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7441 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7501 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7561 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     7621 agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct
     7681 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     7741 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7801 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7861 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7921 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7981 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8041 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8101 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8161 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8221 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8281 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8341 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8401 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8461 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8521 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8581 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8641 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8701 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8761 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8821 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8881 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8941 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9001 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9061 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9121 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9181 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9241 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9301 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9361 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9421 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9481 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9541 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     9601 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     9661 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     9721 cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9781 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9841 ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9901 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9961 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac
    10021 gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    10081 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
    10141 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tacattcctc
    10201 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
    10261 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
    10321 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
    10381 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
    10441 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
    10501 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
    10561 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
    10621 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
    10681 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
    10741 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    10801 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    10861 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
    10921 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
    10981 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
    11041 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
    11101 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
    11161 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
    11221 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
    11281 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
    11341 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
    11401 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
    11461 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
    11521 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
    11581 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
    11641 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
    11701 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
    11761 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
    11821 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
    11881 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
    11941 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
    12001 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
    12061 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
//
