LOCUS       sequence-250486-       11197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of AXL L423Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-AXL-L423Q
FEATURES             Location/Qualifiers
     source          1..11197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        166..1344
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          397..1335
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1292..1312
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1364..1388
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4077..4101)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4154..4195
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4216..4783
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4233..4785
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4377..4394)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4604..4623
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4797..4799,4800..4802,4803..5516)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4797..4799,4800..4802,4803..5513)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5103..5122)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5450..5471
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5530..6118
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5583..5603)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6001..6012)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6193..6426
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6545..6563)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6631..6735
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6736..6804,6805..7596)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6954..6973)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7767..8355
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8256..8275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8509..8526
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8766..8787
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8802..8807,8808..8825,8826..8832)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8840..8856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8845..8867
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8864..8880
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8864..8880
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8930..8949
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9141..9774
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9821..9946
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10443..10676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10861..10905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccagt ttggactagt cgtgaggctc cggtgcccgt
      181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
      241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
      421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
      481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
      541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
      601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
      661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
      721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     1021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     1081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     1141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     1201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     1261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     1321 tttcttccat ttcaggtgtc gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa
     1381 agcaggcacc atggcgtggc ggtgccccag gatgggcagg gtcccgctgg cctggtgctt
     1441 ggcgctgtgc ggctgggcgt gcatggcccc caggggcacg caggctgaag aaagtccctt
     1501 cgtgggcaac ccagggaata tcacaggtgc ccggggactc acgggcaccc ttcggtgtca
     1561 gctccaggtt cagggagagc cccccgaggt acattggctt cgggatggac agatcctgga
     1621 gctcgcggac agcacccaga cccaggtgcc cctgggtgag gatgaacagg atgactggat
     1681 agtggtcagc cagctcagaa tcacctccct gcagctttcc gacacgggac agtaccagtg
     1741 tttggtgttt ctgggacatc agaccttcgt gtcccagcct ggctatgttg ggctggaggg
     1801 cttgccttac ttcctggagg agcccgaaga caggactgtg gccgccaaca cccccttcaa
     1861 cctgagctgc caagctcagg gacccccaga gcccgtggac ctactctggc tccaggatgc
     1921 tgtccccctg gccacggctc caggtcacgg cccccagcgc agcctgcatg ttccagggct
     1981 gaacaagaca tcctctttct cctgcgaagc ccataacgcc aagggggtca ccacatcccg
     2041 cacagccacc atcacagtgc tcccccagca gccccgtaac ctccacctgg tctcccgcca
     2101 acccacggag ctggaggtgg cttggactcc aggcctgagc ggcatctacc ccctgaccca
     2161 ctgcaccctg caggctgtgc tgtcagacga tgggatgggc atccaggcgg gagaaccaga
     2221 ccccccagag gagcccctca cctcgcaagc atccgtgccc ccccatcagc ttcggctagg
     2281 cagcctccat cctcacaccc cttatcacat ccgcgtggca tgcaccagca gccagggccc
     2341 ctcatcctgg acccactggc ttcctgtgga gacgccggag ggagtgcccc tgggcccccc
     2401 tgagaacatt agtgctacgc ggaatgggag ccaggccttc gtgcattggc aagagccccg
     2461 ggcgcccctg cagggtaccc tgttagggta ccggctggcg tatcaaggcc aggacacccc
     2521 agaggtgcta atggacatag ggctaaggca agaggtgacc ctggagctgc agggggacgg
     2581 gtctgtgtcc aatctgacag tgtgtgtggc agcctacact gctgctgggg atggaccctg
     2641 gagcctccca gtaccccagg aggcctggcg cccagggcaa gcacagccag tccaccagct
     2701 ggtgaaggaa ccttcaactc ctgccttctc gtggccctgg tggtatgtac tgctaggagc
     2761 agtcgtggcc gctgcctgtg tcctcatctt ggctctcttc cttgtccacc ggcgaaagaa
     2821 ggagacccgt tatggagaag tgtttgaacc aacagtggaa agaggtgaac tggtagtcag
     2881 gtaccgcgtg cgcaagtcct acagtcgtcg gaccactgaa gctaccttga acagcttggg
     2941 catcagtgaa gagctgaagg agaagctgcg ggatgtgatg gtggaccggc acaaggtggc
     3001 cctggggaag actctgggag agggagagtt tggagctgtg atggaaggcc agctcaacca
     3061 ggacgactcc atcctcaagg tggctgtgaa gacgatgaag attgccatct gcacgaggtc
     3121 agagctggag gatttcctga gtgaagcggt ctgcatgaag gaatttgacc atcccaacgt
     3181 catgaggctc atcggtgtct gtttccaggg ttctgaacga gagagcttcc cagcacctgt
     3241 ggtcatctta cctttcatga aacatggaga cctacacagc ttcctcctct attcccggct
     3301 cggggaccag ccagtgtacc tgcccactca gatgctagtg aagttcatgg cagacatcgc
     3361 cagtggcatg gagtatctga gtaccaagag attcatacac cgggacctgg cggccaggaa
     3421 ctgcatgctg aatgagaaca tgtccgtgtg tgtggcggac ttcgggctct ccaagaagat
     3481 ctacaatggg gactactacc gccagggacg tatcgccaag atgccagtca agtggattgc
     3541 cattgagagt ctagctgacc gtgtctacac cagcaagagc gatgtgtggt ccttcggggt
     3601 gacaatgtgg gagattgcca caagaggcca aaccccatat ccgggcgtgg agaacagcga
     3661 gatttatgac tatctgcgcc ggggaaatcg cctgaagcag cctgcggact gtctggatgg
     3721 actgtatgcc ttgatgtcgc ggtgctggga gctaaatccc caggaccggc caagttttac
     3781 agagctgcgg gaagatttgg agaacacact gaaggccttg cctcctgccc aggagcctga
     3841 cgaaatcctc tatgtcaaca tggatgaggg tggaggttat cctgaacccc ctggagctgc
     3901 aggaggagct gaccccccaa cccagccaga ccctaaggat tcctgtagct gcctcactgc
     3961 ggctgaggtc catcctgctg gacgctatgt cctctgccct tccacaaccc ctagccccgc
     4021 tcagcctgct gataggggct ccccagcagc cccagggcag gaggatggtg cctagaaccc
     4081 agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg
     4141 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
     4201 cggttagtaa tgagatccct cccccccccc taacgttact ggccgaagcc gcttggaata
     4261 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     4321 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     4381 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     4441 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     4501 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     4561 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     4621 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     4681 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     4741 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acacatatgg
     4801 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4861 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     4921 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4981 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     5041 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     5101 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     5161 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     5221 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     5281 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     5341 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     5401 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     5461 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaatcg
     5521 atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5581 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5641 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5701 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5761 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5821 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5881 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     5941 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6001 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6061 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     6121 gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6181 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     6241 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6301 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6361 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6421 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6481 tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga
     6541 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     6601 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6661 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6721 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6781 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6841 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6901 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6961 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     7021 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7081 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7141 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7201 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7261 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7321 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7381 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7441 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7501 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7561 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7621 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7681 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7741 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7801 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7861 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7921 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7981 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8041 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8101 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8161 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8221 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8281 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8341 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8401 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8461 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8521 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     8581 cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8641 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     8701 gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8761 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8821 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     8881 atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8941 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     9001 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
     9061 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
     9121 tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct
     9181 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     9241 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     9301 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     9361 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     9421 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     9481 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     9541 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     9601 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9661 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9721 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     9781 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     9841 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     9901 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     9961 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
    10021 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
    10081 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
    10141 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
    10201 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
    10261 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
    10321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    10381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    10441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    10501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    10561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    10621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    10681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    10741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    10801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    10861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    10921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    10981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    11041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    11101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
    11161 tatcgccgaa ttcacaaatg gcagtattca tccacaa
//
