LOCUS       sequence-250488-       11197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of AXL Q83R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-AXL-Q83R
FEATURES             Location/Qualifiers
     source          1..11197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3912..3936)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3989..4030
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4051..4618
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4068..4620
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4212..4229)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4439..4458
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4632..4634,4635..4637,4638..5351)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4632..4634,4635..4637,4638..5348)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4938..4957)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5285..5306
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5365..5953
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5418..5438)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5836..5847)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6028..6261
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6380..6398)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6466..6570
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6571..6639,6640..7431)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6789..6808)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7602..8190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8091..8110
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8344..8361
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8601..8622
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8637..8642,8643..8660,8661..8667)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8675..8691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8680..8702
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8699..8715
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8699..8715
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8765..8784
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8976..9609
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9656..9781
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10278..10511
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10696..10740
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11033..11150
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccctgc agatatcaac
     1201 aagtttgtac aaaaaagcag gcaccatggc gtggcggtgc cccaggatgg gcagggtccc
     1261 gctggcctgg tgcttggcgc tgtgcggctg ggcgtgcatg gcccccaggg gcacgcaggc
     1321 tgaagaaagt cccttcgtgg gcaacccagg gaatatcaca ggtgcccggg gactcacggg
     1381 cacccttcgg tgtcagctcc aggttcaggg agagcccccc gaggtacatt ggcttcggga
     1441 tggacagatc ctggagctcg cggacagcac ccggacccag gtgcccctgg gtgaggatga
     1501 acaggatgac tggatagtgg tcagccagct cagaatcacc tccctgcagc tttccgacac
     1561 gggacagtac cagtgtttgg tgtttctggg acatcagacc ttcgtgtccc agcctggcta
     1621 tgttgggctg gagggcttgc cttacttcct ggaggagccc gaagacagga ctgtggccgc
     1681 caacaccccc ttcaacctga gctgccaagc tcagggaccc ccagagcccg tggacctact
     1741 ctggctccag gatgctgtcc ccctggccac ggctccaggt cacggccccc agcgcagcct
     1801 gcatgttcca gggctgaaca agacatcctc tttctcctgc gaagcccata acgccaaggg
     1861 ggtcaccaca tcccgcacag ccaccatcac agtgctcccc cagcagcccc gtaacctcca
     1921 cctggtctcc cgccaaccca cggagctgga ggtggcttgg actccaggcc tgagcggcat
     1981 ctaccccctg acccactgca ccctgcaggc tgtgctgtca gacgatggga tgggcatcca
     2041 ggcgggagaa ccagaccccc cagaggagcc cctcacctcg caagcatccg tgccccccca
     2101 tcagcttcgg ctaggcagcc tccatcctca caccccttat cacatccgcg tggcatgcac
     2161 cagcagccag ggcccctcat cctggaccca ctggcttcct gtggagacgc cggagggagt
     2221 gcccctgggc ccccctgaga acattagtgc tacgcggaat gggagccagg ccttcgtgca
     2281 ttggcaagag ccccgggcgc ccctgcaggg taccctgtta gggtaccggc tggcgtatca
     2341 aggccaggac accccagagg tgctaatgga catagggcta aggcaagagg tgaccctgga
     2401 gctgcagggg gacgggtctg tgtccaatct gacagtgtgt gtggcagcct acactgctgc
     2461 tggggatgga ccctggagcc tcccagtacc cctggaggcc tggcgcccag ggcaagcaca
     2521 gccagtccac cagctggtga aggaaccttc aactcctgcc ttctcgtggc cctggtggta
     2581 tgtactgcta ggagcagtcg tggccgctgc ctgtgtcctc atcttggctc tcttccttgt
     2641 ccaccggcga aagaaggaga cccgttatgg agaagtgttt gaaccaacag tggaaagagg
     2701 tgaactggta gtcaggtacc gcgtgcgcaa gtcctacagt cgtcggacca ctgaagctac
     2761 cttgaacagc ttgggcatca gtgaagagct gaaggagaag ctgcgggatg tgatggtgga
     2821 ccggcacaag gtggccctgg ggaagactct gggagaggga gagtttggag ctgtgatgga
     2881 aggccagctc aaccaggacg actccatcct caaggtggct gtgaagacga tgaagattgc
     2941 catctgcacg aggtcagagc tggaggattt cctgagtgaa gcggtctgca tgaaggaatt
     3001 tgaccatccc aacgtcatga ggctcatcgg tgtctgtttc cagggttctg aacgagagag
     3061 cttcccagca cctgtggtca tcttaccttt catgaaacat ggagacctac acagcttcct
     3121 cctctattcc cggctcgggg accagccagt gtacctgccc actcagatgc tagtgaagtt
     3181 catggcagac atcgccagtg gcatggagta tctgagtacc aagagattca tacaccggga
     3241 cctggcggcc aggaactgca tgctgaatga gaacatgtcc gtgtgtgtgg cggacttcgg
     3301 gctctccaag aagatctaca atggggacta ctaccgccag ggacgtatcg ccaagatgcc
     3361 agtcaagtgg attgccattg agagtctagc tgaccgtgtc tacaccagca agagcgatgt
     3421 gtggtccttc ggggtgacaa tgtgggagat tgccacaaga ggccaaaccc catatccggg
     3481 cgtggagaac agcgagattt atgactatct gcgccgggga aatcgcctga agcagcctgc
     3541 ggactgtctg gatggactgt atgccttgat gtcgcggtgc tgggagctaa atccccagga
     3601 ccggccaagt tttacagagc tgcgggaaga tttggagaac acactgaagg ccttgcctcc
     3661 tgcccaggag cctgacgaaa tcctctatgt caacatggat gagggtggag gttatcctga
     3721 accccctgga gctgcaggag gagctgaccc cccaacccag ccagacccta aggattcctg
     3781 tagctgcctc actgcggctg aggtccatcc tgctggacgc tatgtcctct gcccttccac
     3841 aacccctagc cccgctcagc ctgctgatag gggctcccca gcagccccag ggcaggagga
     3901 tggtgcctag aacccagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg
     3961 ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     4021 cgattctacg cgtaccggtt agtaatgaga tccctccccc ccccctaacg ttactggccg
     4081 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     4141 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     4201 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     4261 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     4321 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     4381 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     4441 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     4501 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     4561 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     4621 tggccacaca tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     4681 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     4741 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     4801 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     4861 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     4921 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4981 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     5041 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     5101 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     5161 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     5221 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     5281 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     5341 tgtacaagta aatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg
     5401 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5461 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5521 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5581 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5641 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5701 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5761 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5821 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5881 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5941 gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta
     6001 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     6061 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6121 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6181 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6241 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     6301 ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat
     6361 cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     6421 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6481 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6541 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6601 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6661 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6721 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6781 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     6841 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6901 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6961 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7021 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7081 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     7141 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7201 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7261 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7321 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7381 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7441 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7501 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7561 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7621 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7681 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7741 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7801 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7861 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7921 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7981 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8041 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8101 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8161 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8221 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8281 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8341 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8401 gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg
     8461 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8521 gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg
     8581 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8641 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     8701 ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc actgcattct
     8761 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     8821 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     8881 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     8941 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
     9001 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     9061 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     9121 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     9181 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     9241 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     9301 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     9361 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     9421 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9481 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9541 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9601 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     9661 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     9721 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     9781 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     9841 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     9901 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     9961 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
    10021 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
    10081 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
    10141 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    10201 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    10261 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    10321 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    10381 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    10441 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    10501 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    10561 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    10621 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    10681 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    10741 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    10801 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    10861 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    10921 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    10981 aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac aattttaaaa
    11041 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
    11101 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
    11161 acagggacag cagagatcca gtttggacta gtcgtga
//
