LOCUS       sequence-250489-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR D256Y.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-D256Y
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    221..338
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        386..1564
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          617..1555
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1512..1532
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1584..1608
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1611..1682,1683..3545,3546..3614,3615..5243)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRYEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5245..5269)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5277..5295
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5320..5339)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5321..5339)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5345..5365)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5419..5460
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5481..6048
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5498..6050
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5642..5659)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5869..5888
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6062..6661
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6062..6081)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6558..6578
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6675..7263
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6728..6748)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7146..7157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7338..7571
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7690..7708)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7776..7880
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7881..7949,7950..8741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8912..9500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9401..9420
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9654..9671
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9911..9932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9947..9952,9953..9970,9971..9977)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9985..10001
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9990..10012
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10009..10025
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10009..10025
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10075..10094
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10286..10919
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10966..11091
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11588..11821
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12006..12050
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
       61 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
      121 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
      181 cggtatcgcc gaattcacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
      241 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      301 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      361 gagatccagt ttggactagt cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc
      421 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
      481 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
      541 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
      601 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
      661 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
      721 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
      781 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
      841 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
      901 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
      961 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
     1021 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
     1081 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
     1141 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
     1201 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
     1261 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     1321 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     1381 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     1441 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     1501 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     1561 gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa agcaggcacc atgcgaccct
     1621 ccgggacggc cggggcagcg ctcctggcgc tgctggctgc gctctgcccg gcgagtcggg
     1681 ctctggagga aaagaaagtt tgccaaggca cgagtaacaa gctcacgcag ttgggcactt
     1741 ttgaagatca ttttctcagc ctccagagga tgttcaataa ctgtgaggtg gtccttggga
     1801 atttggaaat tacctatgtg cagaggaatt atgatctttc cttcttaaag accatccagg
     1861 aggtggctgg ttatgtcctc attgccctca acacagtgga gcgaattcct ttggaaaacc
     1921 tgcagatcat cagaggaaat atgtactacg aaaattccta tgccttagca gtcttatcta
     1981 actatgatgc aaataaaacc ggactgaagg agctgcccat gagaaattta caggaaatcc
     2041 tgcatggcgc cgtgcggttc agcaacaacc ctgccctgtg caacgtggag agcatccagt
     2101 ggcgggacat agtcagcagt gactttctca gcaacatgtc gatggacttc cagaaccacc
     2161 tgggcagctg ccaaaagtgt gatccaagct gtcccaatgg gagctgctgg ggtgcaggag
     2221 aggagaactg ccagaaactg accaaaatca tctgtgccca gcagtgctcc gggcgctgcc
     2281 gtggcaagtc ccccagtgac tgctgccaca accagtgtgc tgcaggctgc acaggccccc
     2341 gggagagcga ctgcctggtc tgccgcaaat tccgatacga agccacgtgc aaggacacct
     2401 gccccccact catgctctac aaccccacca cgtaccagat ggatgtgaac cccgagggca
     2461 aatacagctt tggtgccacc tgcgtgaaga agtgtccccg taattatgtg gtgacagatc
     2521 acggctcgtg cgtccgagcc tgtggggccg acagctatga gatggaggaa gacggcgtcc
     2581 gcaagtgtaa gaagtgcgaa gggccttgcc gcaaagtgtg taacggaata ggtattggtg
     2641 aatttaaaga ctcactctcc ataaatgcta cgaatattaa acacttcaaa aactgcacct
     2701 ccatcagtgg cgatctccac atcctgccgg tggcatttag gggtgactcc ttcacacata
     2761 ctcctcctct ggatccacag gaactggata ttctgaaaac cgtaaaggaa atcacagggt
     2821 ttttgctgat tcaggcttgg cctgaaaaca ggacggacct ccatgccttt gagaacctag
     2881 aaatcatacg cggcaggacc aagcaacatg gtcagttttc tcttgcagtc gtcagcctga
     2941 acataacatc cttgggatta cgctccctca aggagataag tgatggagat gtgataattt
     3001 caggaaacaa aaatttgtgc tatgcaaata caataaactg gaaaaaactg tttgggacct
     3061 ccggtcagaa aaccaaaatt ataagcaaca gaggtgaaaa cagctgcaag gccacaggcc
     3121 aggtctgcca tgccttgtgc tcccccgagg gctgctgggg cccggagccc agggactgcg
     3181 tctcttgccg gaatgtcagc cgaggcaggg aatgcgtgga caagtgcaac cttctggagg
     3241 gtgagccaag ggagtttgtg gagaactctg agtgcataca gtgccaccca gagtgcctgc
     3301 ctcaggccat gaacatcacc tgcacaggac ggggaccaga caactgtatc cagtgtgccc
     3361 actacattga cggcccccac tgcgtcaaga cctgcccggc aggagtcatg ggagaaaaca
     3421 acaccctggt ctggaagtac gcagacgccg gccatgtgtg ccacctgtgc catccaaact
     3481 gcacctacgg atgcactggg ccaggtcttg aaggctgtcc aacgaatggg cctaagatcc
     3541 cgtccatcgc cactgggatg gtgggggccc tcctcttgct gctggtggtg gccctgggga
     3601 tcggcctctt catgcgaagg cgccacatcg ttcggaagcg cacgctgcgg aggctgctgc
     3661 aggagaggga gcttgtggag cctcttacac ccagtggaga agctcccaac caagctctct
     3721 tgaggatctt gaaggaaact gaattcaaaa agatcaaagt gctgggctcc ggtgcgttcg
     3781 gcacggtgta taagggactc tggatcccag aaggtgagaa agttaaaatt cccgtcgcta
     3841 tcaaggaatt aagagaagca acatctccga aagccaacaa ggaaatcctc gatgaagcct
     3901 acgtgatggc cagcgtggac aacccccacg tgtgccgcct gctgggcatc tgcctcacct
     3961 ccaccgtgca gctcatcacg cagctcatgc ccttcggctg cctcctggac tatgtccggg
     4021 aacacaaaga caatattggc tcccagtacc tgctcaactg gtgtgtgcag atcgcaaagg
     4081 gcatgaacta cttggaggac cgtcgcttgg tgcaccgcga cctggcagcc aggaacgtac
     4141 tggtgaaaac accgcagcat gtcaagatca cagattttgg gctggccaaa ctgctgggtg
     4201 cggaagagaa agaataccat gcagaaggag gcaaagtgcc tatcaagtgg atggcattgg
     4261 aatcaatttt acacagaatc tatacccacc agagtgatgt ctggagctac ggggtgaccg
     4321 tttgggagtt gatgaccttt ggatccaagc catatgacgg aatccctgcc agcgagatct
     4381 cctccatcct ggagaaagga gaacgcctcc ctcagccacc catatgtacc atcgatgtct
     4441 acatgatcat ggtcaagtgc tggatgatag acgcagatag tcgcccaaag ttccgtgagt
     4501 tgatcatcga attctccaaa atggcccgag acccccagcg ctaccttgtc attcaggggg
     4561 atgaaagaat gcatttgcca agtcctacag actccaactt ctaccgtgcc ctgatggatg
     4621 aagaagacat ggacgacgtg gtggatgccg acgagtacct catcccacag cagggcttct
     4681 tcagcagccc ctccacgtca cggactcccc tcctgagctc tctgagtgca accagcaaca
     4741 attccaccgt ggcttgcatt gatagaaatg ggctgcaaag ctgtcccatc aaggaagaca
     4801 gcttcttgca gcgatacagc tcagacccca caggcgcctt gactgaggac agcatagacg
     4861 acaccttcct cccagtgcct gaatacataa accagtccgt tcccaaaagg cccgctggct
     4921 ctgtgcagaa tcctgtctat cacaatcagc ctctgaaccc cgcgcccagc agagacccac
     4981 actaccagga cccccacagc actgcagtgg gcaaccccga gtatctcaac actgtccagc
     5041 ccacctgtgt caacagcaca ttcgacagcc ctgcccactg ggcccagaaa ggcagccacc
     5101 aaattagcct ggacaaccct gactaccagc aggacttctt tcccaaggaa gccaagccaa
     5161 atggcatctt taagggctcc acagctgaaa atgcagaata cctaagggtc gcgccacaaa
     5221 gcagtgaatt tattggagca tagaacccag ctttcttgta caaagtggtg atatccaatt
     5281 aaccctcact aaagggtgta aatgtttgag atgtatattc cctatagtga gtcgtattac
     5341 cacccaactt ttctatacaa agtggttgat atccagcaca gtggcggccg ctcgagtcta
     5401 gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
     5461 cgtaccggtt agtaatgaga tccctccccc ccccctaacg ttactggccg aagccgcttg
     5521 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     5581 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     5641 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     5701 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     5761 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     5821 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     5881 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     5941 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     6001 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaca
     6061 tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     6121 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     6181 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6241 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6301 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6361 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6421 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6481 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6541 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6601 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta
     6661 aatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6721 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6781 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6841 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6901 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6961 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7021 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     7081 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7141 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7201 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7261 cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7321 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     7381 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7441 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7501 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7561 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7621 atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa
     7681 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     7741 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7981 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8041 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8101 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     8161 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8221 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8281 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8341 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8401 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8461 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8521 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8581 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8641 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8701 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8761 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8821 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8881 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8941 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9001 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9061 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9121 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9181 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9241 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9301 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9361 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9421 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9481 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9541 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9601 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9661 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9721 cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga
     9781 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9841 cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     9901 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     9961 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    10021 atgacatgat tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10081 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    10141 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    10201 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    10261 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
    10321 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
    10381 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    10441 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
    10501 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
    10561 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
    10621 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
    10681 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
    10741 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10801 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10861 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    10921 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    10981 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    11041 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    11101 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    11161 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    11221 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    11281 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    11341 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    11401 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
    11461 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
    11521 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
    11581 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
    11641 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
    11701 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
    11761 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
    11821 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
    11881 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
    11941 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
    12001 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
    12061 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
    12121 ta
//
