LOCUS       sequence-250502-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR E709A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-E709A
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    110..227
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        275..1453
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          506..1444
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1401..1421
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1473..1497
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1500..1571,1572..3434,3435..3503,3504..5132)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKATEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5134..5158)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5166..5184
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5209..5228)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5210..5228)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5234..5254)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5308..5349
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5370..5937
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5387..5939
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5531..5548)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5758..5777
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5951..6550
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5951..5970)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6447..6467
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6564..7152
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6617..6637)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7035..7046)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7227..7460
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7579..7597)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7665..7769
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7770..7838,7839..8630)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8801..9389
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9290..9309
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9543..9560
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9800..9821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9836..9841,9842..9859,9860..9866)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9874..9890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9879..9901
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9898..9914
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9898..9914
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9964..9983
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10175..10808
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10855..10980
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11477..11710
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11895..11939
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
       61 ggatctcgac ggtatcgccg aattcacaaa tggcagtatt catccacaat tttaaaagaa
      121 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
      181 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
      241 gggacagcag agatccagtt tggactagtc gtgaggctcc ggtgcccgtc agtgggcaga
      301 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
      361 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
      421 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
      481 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
      541 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
      601 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
      661 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
      721 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
      781 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
      841 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
      901 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
      961 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     1021 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     1081 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     1141 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     1201 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     1261 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     1321 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     1381 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     1441 tcaggtgtcg tgaagcggcc ctgcagatat caacaagttt gtacaaaaaa gcaggcacca
     1501 tgcgaccctc cgggacggcc ggggcagcgc tcctggcgct gctggctgcg ctctgcccgg
     1561 cgagtcgggc tctggaggaa aagaaagttt gccaaggcac gagtaacaag ctcacgcagt
     1621 tgggcacttt tgaagatcat tttctcagcc tccagaggat gttcaataac tgtgaggtgg
     1681 tccttgggaa tttggaaatt acctatgtgc agaggaatta tgatctttcc ttcttaaaga
     1741 ccatccagga ggtggctggt tatgtcctca ttgccctcaa cacagtggag cgaattcctt
     1801 tggaaaacct gcagatcatc agaggaaata tgtactacga aaattcctat gccttagcag
     1861 tcttatctaa ctatgatgca aataaaaccg gactgaagga gctgcccatg agaaatttac
     1921 aggaaatcct gcatggcgcc gtgcggttca gcaacaaccc tgccctgtgc aacgtggaga
     1981 gcatccagtg gcgggacata gtcagcagtg actttctcag caacatgtcg atggacttcc
     2041 agaaccacct gggcagctgc caaaagtgtg atccaagctg tcccaatggg agctgctggg
     2101 gtgcaggaga ggagaactgc cagaaactga ccaaaatcat ctgtgcccag cagtgctccg
     2161 ggcgctgccg tggcaagtcc cccagtgact gctgccacaa ccagtgtgct gcaggctgca
     2221 caggcccccg ggagagcgac tgcctggtct gccgcaaatt ccgagacgaa gccacgtgca
     2281 aggacacctg ccccccactc atgctctaca accccaccac gtaccagatg gatgtgaacc
     2341 ccgagggcaa atacagcttt ggtgccacct gcgtgaagaa gtgtccccgt aattatgtgg
     2401 tgacagatca cggctcgtgc gtccgagcct gtggggccga cagctatgag atggaggaag
     2461 acggcgtccg caagtgtaag aagtgcgaag ggccttgccg caaagtgtgt aacggaatag
     2521 gtattggtga atttaaagac tcactctcca taaatgctac gaatattaaa cacttcaaaa
     2581 actgcacctc catcagtggc gatctccaca tcctgccggt ggcatttagg ggtgactcct
     2641 tcacacatac tcctcctctg gatccacagg aactggatat tctgaaaacc gtaaaggaaa
     2701 tcacagggtt tttgctgatt caggcttggc ctgaaaacag gacggacctc catgcctttg
     2761 agaacctaga aatcatacgc ggcaggacca agcaacatgg tcagttttct cttgcagtcg
     2821 tcagcctgaa cataacatcc ttgggattac gctccctcaa ggagataagt gatggagatg
     2881 tgataatttc aggaaacaaa aatttgtgct atgcaaatac aataaactgg aaaaaactgt
     2941 ttgggacctc cggtcagaaa accaaaatta taagcaacag aggtgaaaac agctgcaagg
     3001 ccacaggcca ggtctgccat gccttgtgct cccccgaggg ctgctggggc ccggagccca
     3061 gggactgcgt ctcttgccgg aatgtcagcc gaggcaggga atgcgtggac aagtgcaacc
     3121 ttctggaggg tgagccaagg gagtttgtgg agaactctga gtgcatacag tgccacccag
     3181 agtgcctgcc tcaggccatg aacatcacct gcacaggacg gggaccagac aactgtatcc
     3241 agtgtgccca ctacattgac ggcccccact gcgtcaagac ctgcccggca ggagtcatgg
     3301 gagaaaacaa caccctggtc tggaagtacg cagacgccgg ccatgtgtgc cacctgtgcc
     3361 atccaaactg cacctacgga tgcactgggc caggtcttga aggctgtcca acgaatgggc
     3421 ctaagatccc gtccatcgcc actgggatgg tgggggccct cctcttgctg ctggtggtgg
     3481 ccctggggat cggcctcttc atgcgaaggc gccacatcgt tcggaagcgc acgctgcgga
     3541 ggctgctgca ggagagggag cttgtggagc ctcttacacc cagtggagaa gctcccaacc
     3601 aagctctctt gaggatcttg aaggcaactg aattcaaaaa gatcaaagtg ctgggctccg
     3661 gtgcgttcgg cacggtgtat aagggactct ggatcccaga aggtgagaaa gttaaaattc
     3721 ccgtcgctat caaggaatta agagaagcaa catctccgaa agccaacaag gaaatcctcg
     3781 atgaagccta cgtgatggcc agcgtggaca acccccacgt gtgccgcctg ctgggcatct
     3841 gcctcacctc caccgtgcag ctcatcacgc agctcatgcc cttcggctgc ctcctggact
     3901 atgtccggga acacaaagac aatattggct cccagtacct gctcaactgg tgtgtgcaga
     3961 tcgcaaaggg catgaactac ttggaggacc gtcgcttggt gcaccgcgac ctggcagcca
     4021 ggaacgtact ggtgaaaaca ccgcagcatg tcaagatcac agattttggg ctggccaaac
     4081 tgctgggtgc ggaagagaaa gaataccatg cagaaggagg caaagtgcct atcaagtgga
     4141 tggcattgga atcaatttta cacagaatct atacccacca gagtgatgtc tggagctacg
     4201 gggtgaccgt ttgggagttg atgacctttg gatccaagcc atatgacgga atccctgcca
     4261 gcgagatctc ctccatcctg gagaaaggag aacgcctccc tcagccaccc atatgtacca
     4321 tcgatgtcta catgatcatg gtcaagtgct ggatgataga cgcagatagt cgcccaaagt
     4381 tccgtgagtt gatcatcgaa ttctccaaaa tggcccgaga cccccagcgc taccttgtca
     4441 ttcaggggga tgaaagaatg catttgccaa gtcctacaga ctccaacttc taccgtgccc
     4501 tgatggatga agaagacatg gacgacgtgg tggatgccga cgagtacctc atcccacagc
     4561 agggcttctt cagcagcccc tccacgtcac ggactcccct cctgagctct ctgagtgcaa
     4621 ccagcaacaa ttccaccgtg gcttgcattg atagaaatgg gctgcaaagc tgtcccatca
     4681 aggaagacag cttcttgcag cgatacagct cagaccccac aggcgccttg actgaggaca
     4741 gcatagacga caccttcctc ccagtgcctg aatacataaa ccagtccgtt cccaaaaggc
     4801 ccgctggctc tgtgcagaat cctgtctatc acaatcagcc tctgaacccc gcgcccagca
     4861 gagacccaca ctaccaggac ccccacagca ctgcagtggg caaccccgag tatctcaaca
     4921 ctgtccagcc cacctgtgtc aacagcacat tcgacagccc tgcccactgg gcccagaaag
     4981 gcagccacca aattagcctg gacaaccctg actaccagca ggacttcttt cccaaggaag
     5041 ccaagccaaa tggcatcttt aagggctcca cagctgaaaa tgcagaatac ctaagggtcg
     5101 cgccacaaag cagtgaattt attggagcat agaacccagc tttcttgtac aaagtggtga
     5161 tatccaatta accctcacta aagggttatg tagagatgaa agaaattacc ctatagtgag
     5221 tcgtattacc acccaacttt tctatacaaa gtggttgata tccagcacag tggcggccgc
     5281 tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc
     5341 gattctacgc gtaccggtta gtaatgagat ccctcccccc cccctaacgt tactggccga
     5401 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     5461 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     5521 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     5581 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     5641 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     5701 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     5761 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     5821 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     5881 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat
     5941 ggccacacat atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     6001 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     6061 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     6121 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     6181 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     6241 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     6301 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     6361 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     6421 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     6481 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     6541 cggtgcctaa atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga
     6601 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6661 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6721 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6781 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6841 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6901 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     6961 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7021 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7081 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7141 ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag
     7201 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     7261 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7321 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7381 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7441 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7501 tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc
     7561 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     7621 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7681 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7741 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7801 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7861 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7921 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7981 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     8041 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8101 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8161 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8221 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8281 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8341 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8401 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8461 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8521 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8581 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8641 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8701 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8761 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8821 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8881 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8941 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9001 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9061 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9121 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9181 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9241 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9301 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9361 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9421 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9481 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9541 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     9601 cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc
     9661 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9721 aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg
     9781 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     9841 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     9901 gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta
     9961 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
    10021 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
    10081 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
    10141 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
    10201 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
    10261 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
    10321 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
    10381 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
    10441 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
    10501 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
    10561 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
    10621 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
    10681 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
    10741 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
    10801 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
    10861 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
    10921 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
    10981 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
    11041 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
    11101 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
    11161 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
    11221 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
    11281 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
    11341 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
    11401 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
    11461 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
    11521 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
    11581 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
    11641 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
    11701 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
    11761 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
    11821 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
    11881 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
    11941 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
    12001 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
    12061 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
    12121 cc
//
